Gelatin Verona Buffer (GVB) ａnd Verona Buffered Saline (VBS) are essential buffers for complement research. Compared to Gelatin Verona Buffer (GVB), Verona Buffered Saline (VBS) lacks gelatin components.

Gelatin Verona Buffer (GVB):

B102-250mL; B100-1L

Name GVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3 Containing 0.15mM calcium chloride ａnd 0.5mM magnesium chloride

The application should use GVB++to prepare antibody sensitized sheep red blood cells (EA) ａnd clean them before use for testing; GVB++can be used to dilute all components of complement classical pathway ａnd lectin pathway experiments.

B103-250mL; B101-1L

Name GVBo (GVB without Ca++and Mg++) Gelatin Flora Buffer without Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3

Apply GVBo to prepare rabbit red blood cells (Er) ａnd clean them before use for assay; GVBo can be used to dilute all components of the complement bypass pathway test.

B104-250mL; B105-1L

Name GVBE (GVBo with EDTA) Gelatin Flora Buffer with EDTA

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3

The application of GVBE is usually used as a buffer added at the end of the assay to prevent complement activation ａnd dilute the reaction to a level that can be read by spectrophotometry. It is also used to establish a control experiment where complement activation should not occur.

B110-250mL; B111-1L

Name SGVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Sucrose, Ca++, ａnd Mg++

Ingredients: 170mM Sucrose, 0.1% gelatin, 5mM Vernal, 57mM NaCl, 0.025% NaN3, 0.15mM calcium chloride, ａnd 0.5mM magnesium chloride, pH 7.3

SGVB++can be used to detect C1 in purified C1 complex (product number A098) ａnd normal human serum samples. In these experiments, all components were diluted in SGVB++and EA cells were washed into this buffer. In binding experiments, such as detecting the binding of C5, factor B, or factor H to EsC3b cells (sheep red blood cells with surface bound C3b) or ZymosanC3b cells, it also benefits from being performed in SGVB++. The combination of SGVB++can be enhanced as much as 10 times compared with the use of buffer solutions with physiological ionic strength (such as GVB or PBS).

明膠弗洛拿緩沖液(Gelatin Veronal Buffer, GVB)和弗洛拿緩沖的生理鹽水(Veronal Buffered Saline, VBS)是補(bǔ)體研究的必備緩沖液, 弗洛拿緩沖的生理鹽水(VBS)和明膠弗洛拿緩沖液(GVB)相比缺少明膠組分。   

艾美捷多種明膠弗洛拿緩沖液(Gelatin Veronal Buffer, GVB)：

編號 B102-250mL; B100-1L

名稱 GVB++ (with Ca++ ａnd Mg++)含Ca++和Mg++的明膠弗洛拿緩沖液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3. Containing 0.15mM calcium chloride ａnd 0.5mM magnesium chloride

應(yīng)用 應(yīng)使用GVB++制備抗體致敏羊紅細(xì)胞(EA)，并在用于測定對其進(jìn)行清洗；GVB++可用于稀釋補(bǔ)體經(jīng)典途徑和凝集素途徑試驗的所有成分。

編號 B103-250mL; B101-1L

名稱 GVBo (GVB without Ca++ ａnd Mg++)不含Ca++和Mg++的明膠弗洛拿緩沖液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3.

應(yīng)用 GVBo應(yīng)用于制備兔紅細(xì)胞(Er)，并在用于測定對其進(jìn)行清洗; GVBo可用于稀釋補(bǔ)體旁路途徑試驗的所有組分。

編號 B104-250mL; B105-1L

名稱 GVBE (GVBo with EDTA)含EDTA的明膠弗洛拿緩沖液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3.

應(yīng)用 GVBE通常用作在測定結(jié)束時添加的緩沖液，以阻止補(bǔ)體活化并將反應(yīng)稀釋到可分光光度法讀取的水平。它也用于建立不應(yīng)發(fā)生補(bǔ)體活化的對照試驗。

編號 B110-250mL; B111-1L

名稱 SGVB++ (with Ca++ ａnd Mg++)含蔗糖，Ca++和Mg++的明膠弗洛拿緩沖液

成分 170mM Sucrose, 0.1% gelatin, 5mM Veronal, 57mM NaCl, 0.025% NaN3 , 0.15mM calcium chloride, ａnd 0.5mM magnesium chloride, pH7.3.

應(yīng)用 SGVB++可用于檢測純化的C1復(fù)合物(貨號A098)和正常人血清樣品中的C1。在這些實驗中，所有成分在SGVB++中稀釋，并將EA細(xì)胞沖洗到該緩沖液中。在結(jié)合實驗中，如檢測C5、因子B或因子H與EsC3b細(xì)胞(帶有表面結(jié)合的C3b的綿羊紅細(xì)胞)或ZymosanC3b細(xì)胞的結(jié)合，也受益于在SGVB++中進(jìn)行。SGVB++與使用具有生理離子強(qiáng)度的緩沖液(如GVB或PBS)相比，結(jié)合的增強(qiáng)可多達(dá)10倍。

來源：https://www.amyjet.com/brand/gvb.shtml