Aimijie Gelatin Flora Buffer with EDTA:

Cat B104-250mL;  B105-1L

Name GVBE (GVBo with EDTA) Gelatin Flora Buffer with EDTA

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3

The application of GVBE is usually used as a buffer added at the end of the assay to prevent complement activation ａnd dilute the reaction to a level that can be read by spectrophotometry. It is also used to establish a control experiment where complement activation should not occur.

(GVBE) (glucose Flora buffer with EDTA) The pH buffer shows a significant difference from the normal value, ａnd the instrument needs to be calibrated using a standard buffer solution according to the instrument instructions. The calibration method is as follows:

1) Connect the composite electrode after preheating the instrument:

2) Turn the selector switch to the PH position:

3) Adjust the temperature compensation knob white line to align with the solution temperature value (room temperature):

4) Turn the slope adjustment knob all the way clockwise:

5) Insert the cleaned electrode into a standard pH buffer solution:

6) Adjust the positioning knob to match the instrument reading with the pH value of the buffer solution at the current temperature. After each measurement, the electrode must be washed with distilled water ａnd the water on the surface of the electrode must be sucked dry with wiping paper. After normal calibration, there will be no abnormalities in the data when measuring the pH value of the standard buffer solution in reverse.

明膠弗洛拿緩沖液(Gelatin Veronal Buffer, GVB)和弗洛拿緩沖的生理鹽水(Veronal Buffered Saline, VBS)是補(bǔ)體研究的必備緩沖液, 弗洛拿緩沖的生理鹽水(VBS)和明膠弗洛拿緩沖液(GVB)相比缺少明膠組分。   

艾美捷含EDTA的明膠弗洛拿緩沖液：

Cat B104-250mL; B105-1L

名稱 GVBE (GVBo with EDTA)含EDTA的明膠弗洛拿緩沖液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3.

應(yīng)用 GVBE通常用作在測定結(jié)束時添加的緩沖液，以阻止補(bǔ)體活化并將反應(yīng)稀釋到可分光光度法讀取的水平。它也用于建立不應(yīng)發(fā)生補(bǔ)體活化的對照試驗(yàn)。

（GVBE）（帶EDTA的葡萄糖弗洛拿緩沖液） pH緩沖液顯示pH跟正常值差距過大需要按儀器說明用標(biāo)準(zhǔn)緩沖溶液標(biāo)定儀器，標(biāo)定方法如下：

1）儀器預(yù)熱后接上復(fù)合電：

2）將選擇開關(guān)調(diào)到PH檔：

3）調(diào)節(jié)溫度補(bǔ)賞旋鈕白線對準(zhǔn)溶液溫度值（室溫）：

4）斜率調(diào)節(jié)旋鈕順時針旋到底：

5）把清洗過的擦凈的電插入標(biāo)準(zhǔn)PH緩沖溶液中：

6）調(diào)節(jié)定位旋鈕使儀器讀數(shù)與該緩沖溶液當(dāng)時溫度下的PH值一致。每一次測定后都必須用蒸餾水將電洗凈，用擦鏡紙將電表面的水吸干。經(jīng)過正常標(biāo)定后，再反過來測標(biāo)準(zhǔn)緩沖溶液的PH值時，數(shù)據(jù)不會出現(xiàn)異常。

Glucose Veronal Buffer with EDTA（GVBE）（帶EDTA的葡萄糖弗洛拿緩沖液）測定緩沖溶液ph值時為*理論值與測量值誤差較大

看看儀器和樣品，一般不存在可見信和理論值都是未知值，如果經(jīng)常測出現(xiàn)較大差距要先看看。儀器和樣品，使用PH計(jì)時用中性（6.86）定位再根據(jù)被測樣品的酸堿性選擇4.01或9.18的標(biāo)定.之所以用標(biāo)準(zhǔn)溶液定位，原因是我們用已知pH值的緩沖溶液將pH計(jì)校正到該pH下，才會使測定的樣品準(zhǔn)確度高.

來源：https://www.amyjet.com/products/B104.shtml