DNA/RNA preparation by traditional methods frequently contains residual phenol and/or chloroform. The GenElute^™-E Single Spin RNA Cleanup Kit is a one-step cleanup kit for the depletion of organic solvent traces, including phenol, TRIzol reagent, chloroform, ａnd ethanol, from RNA solutions. Ideally suited for a reliable clean-up when a universal preparation for NGS library prep is desired or required, or for RNA cleanup following silica-based isolation to remove process contaminants

GenElute^™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins ａnd organic solvents are removed by the column material ａnd the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, ａnd no use of hazardous materials like chaotropic salts or organic solvents.

With conventional methods, traces of chaotropic salt ａnd ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute^™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as RT-PCR, gene expression, ａnd NGS.

Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 μl. The purified nucleic acid is eluted in water ａnd can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" ａnd "Designing safer chemicals". Click here for more information.

Traditional methods for purifying genomic RNA rely on organic solvents or medium ａnd large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low RNA concentration, ａnd low purity. The GenElute^™-E Single Spin RNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, organic solvents, ａnd dyes from RNA solutions. Purified RNA is eluted in Tris buffer, pH 7.8 ａnd can immediately be used for most common downstream applications, including RT-PCR, genotyping, NGS, ａnd others.

Use within 12 months of receipt.

Fast ａnd efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps ａnd hands-on timeHigher quality RNA: negative chromotography-based purification reduces carryover of RT-PCR inhibitors ａnd other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality ａnd efficacy so your results are not compromised.  GenElute^™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute^™-E is designated a Greener Alternative Product based on the "Prevention" ａnd "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute^™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, ａnd improved recyclability. Packaging is FCS-marked for sustainable forestry practices ａnd produced from greater than 70% recycled content.

GenElute is a trademark of Sigma-Aldrich Co. LLC