確定溶解合成肽的*佳溶劑通常是一個(gè)挑戰(zhàn)。雖然許多肽在水溶液中具有良好的溶解性，但也有其他多肽具有低溶解度和不溶性。當(dāng)多肽具有長(zhǎng)的疏水性氨基酸序列時(shí)，其溶解將會(huì)成為一個(gè)特別的挑戰(zhàn)。以下指南我們將提供一些有助于預(yù)測(cè)肽溶解的建議。

1.It is best to aliquot ａnd test a small quantity of peptide first to determine optimum conditions.

先分裝并進(jìn)行少量多肽的溶解度測(cè)試，以確定*佳溶解條件。

2. Ensure you understand the appropriate concentration at which the peptide should be dissolved. Please see this link to a great molarity calculator（https://www.graphpad.com/quickcalcs/molarityform.cfm） to help you determine the proper concentration. 

     確保您了解多肽溶解的適當(dāng)濃度，請(qǐng)查看 molarity calculator摩爾計(jì)算器（https://www.graphpad.com/quickcalcs/molarityform.cfm），以幫助您計(jì)算確定適當(dāng)?shù)臐舛取?/span>

3. Depending on peptide composition, there are different solvents that are needed to dissolve your lyophilized peptide. Please confirm solubility with 2-3 different sources (publications, suppliers). 

      根據(jù)肽的組成，溶解凍干肽需要不同的溶劑。請(qǐng)確認(rèn)2-3種不同來源（出版物，供應(yīng)商）的溶解度。

4. Some general guidelines include:

1）Peptides shorter than five residues are usually soluble in water or aqueous buffer, except when the entire sequence consists of hydrophobic amino acids (e.g., W, L, I, F, M, V, Y).

2）Hydrophilic peptides containing > 25% charged residues (e.g., D, K, R, H ａnd E) ａnd 25% hydrophobic amino acids are usually soluble in water or aqueous buffers.

3）Peptides containing 50% ａnd more hydrophobic residues might be insoluble or only partly soluble in aqueous solutions.

多肽溶解一般準(zhǔn)則包括：

1）短于5個(gè)殘基的肽通常可溶于水或水性緩沖液，除非整個(gè)序列由疏水性氨基酸（例如W，L，I，F(xiàn)，M，V，Y）組成。

2）含有> 25％帶電殘基（例如D，K，R，H和E）和25％疏水性氨基酸的親水性肽通�？扇苡谒�或水性緩沖液中。

3）含有50％和更多疏水殘基的肽可能不溶或僅部分溶于水溶液。

Please consult literature pertaining to your specific protein as the above are only general guidelines.

具體請(qǐng)參閱有關(guān)您的特定蛋白質(zhì)/多肽的相關(guān)文獻(xiàn)。

5. Warming your solution (< 40°C) may aid in dissolving your peptide. 

加熱溶劑（<40°C）可能有助于溶解你的多肽。

6. The table below may help in determining the characteristics of your particular peptide. 

下表將有助于確定您目的肽的特征。