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雙通道PAM-100熒光儀 Dual-PAM-100

雙通道PAM-100熒光儀 Dual-PAM-100

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相當(dāng)于兩臺(tái)PAM-101/102/103，分別或同步測(cè)量P700(PS I)和葉綠素?zé)晒?PS II)。
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dual-pam-100: 一套空前強(qiáng)大的測(cè)量系統(tǒng)
schreiber教授因發(fā)明pam系列調(diào)制葉綠素?zé)晒鈨x而獲得首屆國(guó)際光合作用協(xié)會(huì)（ispr）創(chuàng)新獎(jiǎng)

1985年開(kāi)始商品化的全世界第一臺(tái)調(diào)制熒光儀pam-100被幾代科學(xué)家所廣泛采用。dual-pam-100相當(dāng)于兩臺(tái)pam-100的功能。一方面，它繼承了pam-100的所有優(yōu)點(diǎn)，可以進(jìn)行復(fù)雜的葉綠素?zé)晒夥治觯╬s ii活性）；另一方面，它還可以通過(guò)測(cè)量p700的吸收變化來(lái)檢測(cè)ps i的活性。特別需要強(qiáng)調(diào)的是，dual-pam-100可以在完全同步的情況下測(cè)量葉綠素?zé)晒夂蚿700吸收變化。此外，通過(guò)特殊的激發(fā)-檢測(cè)單元還可以測(cè)量葉綠體或微藻的許多重要光合參數(shù)，如跨膜質(zhì)子梯度delta ph（通過(guò)9-aa熒光或吖啶黃熒光）、類囊體膜的電勢(shì)（通過(guò)類胡蘿卜素的差示吸收，“p515”）和nadp的氧還狀態(tài)（通過(guò)nadph熒光）等。如果需要極高的靈敏度可以通過(guò)連接光電倍增管檢測(cè)器實(shí)現(xiàn)。



特點(diǎn)：
1）聲譽(yù)卓著的pam-101/102/103的升級(jí)版
2）專業(yè)的dual-pam操作軟件
3）自動(dòng)測(cè)量模式下操作極其簡(jiǎn)單
4）創(chuàng)新的光-電配件使得儀器非常便攜
5）整合式光源（紅光、藍(lán)光、遠(yuǎn)紅光、單脈沖飽和閃光、多脈沖飽和閃光）
6）多版本可選，用于測(cè)量高等植物和微藻
7）利用飽和脈沖法測(cè)量ps i活性
8）同步測(cè)量ps i和ps ii的量子產(chǎn)量
9）可以分析藍(lán)藻的光合作用
10）可選配用于測(cè)量ph、nadph和p515等的附件		功能：
1）ps i和ps ii的光化學(xué)量子產(chǎn)量
2）葉綠素?zé)晒獯銣鐓?shù)
3）系統(tǒng)間電子傳遞動(dòng)力學(xué)
4）系統(tǒng)間電子載體庫(kù)的大�。ㄌ厥獾膕t/mt方法）
5）圍繞ps i的環(huán)式電子傳遞動(dòng)力學(xué)
6）ps i和ps ii能量傳遞的光響應(yīng)曲線
7）熒光快速上升相（線性時(shí)間和對(duì)數(shù)時(shí)間）
8）測(cè)量pq庫(kù)的還原態(tài)
9）測(cè)量光系統(tǒng)ii的吸收截面積

與pam-100相比，dual-pam-100的主要特點(diǎn)：
1）dual-pam-100完全由電腦控制，通過(guò)專業(yè)的windows操作軟件dualpam進(jìn)行。
2）軟件dualpam除了基本的系統(tǒng)操作外，還提供許多特定的測(cè)量程序。
3）所有必需的光源（激發(fā)葉綠素?zé)晒獾募t光和藍(lán)光、測(cè)量p700的近紅外光、紅色和藍(lán)色的光化光、單脈沖與多脈沖飽和閃光、遠(yuǎn)紅光）均整合在基礎(chǔ)系統(tǒng)中，不再需要復(fù)雜的電纜連接。
4）采用了專為dual-pam-100設(shè)計(jì)的許多新光-電配件，使得激發(fā)-檢測(cè)單元和整合式光源非常便攜、非常便于安裝和拆卸。
5）所有的光源都可通過(guò)軟件在2.5 μs的時(shí)間分辨率下控制。
6）測(cè)量光的頻率范圍非常大（1 hz～400 khz），因此同一個(gè)測(cè)量光源既可以用于測(cè)量fo，也可以用于誘發(fā)快速動(dòng)力學(xué)（如熒光快速上升相或閃光弛豫動(dòng)力學(xué)）。
7）用戶可將針對(duì)特殊實(shí)驗(yàn)/樣品的儀器設(shè)置存儲(chǔ)起來(lái)，此后可在完全相同的設(shè)置下重復(fù)實(shí)驗(yàn)。
8）葉綠素?zé)晒夂蚿700的信號(hào)變化完全同步，并且是用同一個(gè)檢測(cè)器檢測(cè)，且不會(huì)互相干擾。
9）測(cè)量藍(lán)藻時(shí)注意：用紅光激發(fā)ps ii的熒光，用藍(lán)光或遠(yuǎn)紅光激發(fā)ps i。
   


       

dual-pam-100的這些特點(diǎn)開(kāi)啟了基礎(chǔ)光合作用研究和應(yīng)用光合作用研究的新途徑。過(guò)去，同步測(cè)量ps i和ps ii的量子產(chǎn)量需要很強(qiáng)的專業(yè)背景和熟練的操作技巧，只有光合作用領(lǐng)域的少數(shù)專家會(huì)這項(xiàng)技術(shù)�，F(xiàn)在，即使是初學(xué)者，也可迅速掌握同步測(cè)量ps i和ps ii活性的技術(shù)，不再需要復(fù)雜的操作技巧。

同步測(cè)量葉綠素?zé)晒猓╬s ii）和p700(ps i)的誘導(dǎo)曲線

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同步測(cè)量葉綠素?zé)晒猓╬s ii）和p700(ps i)的光響應(yīng)曲線


專為dual-pam-100設(shè)計(jì)的windows操作軟件dualpam功能強(qiáng)大，允許獨(dú)立記錄葉綠素?zé)晒饣騪700，或同步記錄葉綠素?zé)晒夂蚿700，記錄每次打開(kāi)飽和脈沖時(shí)的動(dòng)力學(xué)變化，以線性時(shí)間多對(duì)數(shù)時(shí)間記錄快速熒光上升動(dòng)力學(xué)（分辨率2.5 us），分析各種熒光參數(shù)的變化等等。

p700的測(cè)量



以線性時(shí)間記錄的熒光快速上升動(dòng)力學(xué)

以對(duì)數(shù)時(shí)間記錄的熒光快速上升動(dòng)力學(xué)（相當(dāng)于o-j-i-p相）


kramer的新熒光參數(shù)資料，這是一篇2004年發(fā)表在photosynthesis research 上的文章，見(jiàn)如下描述:

kramer提出的新參數(shù)：
ql，用于代替qp
y（ii）＝y(tǒng)＝（fm’-f）/fm’
y（npq) 表征ps ii處過(guò)量光能耗散為熱，與光保護(hù)有關(guān)
y（no） 表征ps ii處過(guò)量光能引起的光損傷
y(ii)+y(npq)+y(no)=1

schreiber教授和其學(xué)生klughammer博士新設(shè)計(jì)的dual-pam-100中，借鑒測(cè)量葉綠素?zé)晒獾姆椒ǎ略隽藥讉�(gè)參數(shù)：
y（i） ps i的量子產(chǎn)量或ps i的光合效率
y（nd) 表征ps i處過(guò)量光能耗散為熱，與光保護(hù)有關(guān)
y（na） 表征ps i處過(guò)量光能引起的光損傷
y(i)+y(nd)+y(na)=1

色素分子處于氧化態(tài)和還原態(tài)時(shí)，或增加/減少亞基后，其吸收峰會(huì)有變化。基于此原理，p700吸收變化、p515吸收變化（類胡蘿卜素能態(tài)）、p505吸收變化（葉黃素循環(huán)）等均可通過(guò)dual-pam-100測(cè)量。主機(jī)共用，只是更換激發(fā)-檢測(cè)單元即可。此外，dual-pam-100還可用于測(cè)量nadph熒光、9aa熒光（跨膜質(zhì)子梯度）等。

部分利用dual-pam-100發(fā)表的p700文獻(xiàn)

1. aronsson h, schöttler ma, kelly aa, sundqvist c, dörmann p, karim s, jarvis p: monogalactosyldiacylglycerol deficiency in arabidopsis affects pigment composition in the prolamellar body and impairs thylakoid membrane energization and photoprotection in leaves. plant physiology 2008;148:580-592.
2. bailey s, melis a, mackey krm, cardol p, finazzi g, dijken gv, berge gm, arrigo k, shrager j, grossman a: alternative photosynthetic electron flow to oxygen in marine synechococcus biochimica et biophysica acta 2008;1777:269-276.
3. ehlert b, schöttler ma, tischendorf g, ludwig-müller j, bock r: the paramutated sulfurea locus of tomato is involved in auxin biosynthesis. journal of experimental biology 2008:in press.
4. ma w, chen l, wei l, wang q: excitation energy transfer between photosystems in the cyanobacterium synechocystis 6803 journal of luminescence 2008;128:546-548.
5. rogalski m, schöttler ma, thiele w, schulze wx, bock r: rpl33, a nonessential plastid-encoded ribosomal protein in tobacco, is required under cold stress conditions. the plant cell 2007;20:2221-2237.
6. schöttler m, flügel c, thiele w, stegemann s, bock r: the plastome-encoded psaj subunit is required for efficient photosystem i excitation, but not for plastocyanin oxidation in tobacco. biochemical journal 2007;403:251-260.
7. volkmer t, schneider d, bernát g, kirchhoff h, wenk s-o, rögner m: ssr2998 of synechocystis sp. pcc 6803 is involved in regulation of cyanobacterial electron transport and associated with the cytochrome b6f complex. journal of biological chemistry 2007;282:3730-3737.
8. lohmann a, schottler ma, brehelin c, kessler f, bock r, cahoon eb, dormann p: deficiency in phylloquinone (vitamin k1) methylation affects prenyl quinone distribution, photosystem i abundance, and anthocyanin accumulation in the arabidopsis atmeng mutant. journal of biological chemistry 2006;281:40461-40472.
9. schöttler ma, flügel c, thiele w, bock r: knock-out of the plastid-encoded petl subunit results in reduced stability and accelerated leaf age-dependent loss of the cytochrome b6f complex. journal of biological chemistry 2006;282:976-985.


部分利用pam-101/102/103發(fā)表的p700文獻(xiàn)

1. wang p, duan w, takabayashi a, endo t, shikanai t, ye j-y, mi h, 2006. chloroplastic nad(p)h dehydrogenase in tobacco leaves functions in alleviation of oxidative damage caused by temperature stress. plant physiology 141: 465-474.
2. xu d, liu x, zhao j, zhao j, 2005. fesm, a membrane iron-sulfur protein, is required for cyclic electron flow around photosystem i and photoheterotrophic growth of the cyanobacterium synechococcus sp. pcc 7002. plant physiology 138: 1586-1595.
3. pikulenko mm, bulychev aa, 2005. light-triggered action potentials and changes in quantum efficiency of photosystem ii in anthoceros cells russian journal of plant physiology 52: 584-590.
4. peterson rb, 2005. psbs genotype in relation to coordinated function of ps ii and ps i in arabidopsis leaves. photosynthesis research 85: 205-219.
5. okegawa y, tsuyama m, kobayashi y, shikanai t, 2005. the pgr1 mutation in the rieske subunit of the cytochrome b6f complex does not affect pgr5-dependent cyclic electron transport around photosystem i. the journal of biological chemistry 280: 28332-28336.
6. munné-bosch s, shikanai t, asada k, 2005. enhanced ferredoxin-dependent cyclic electron flow around photosystem i and α-tocopherol quinone accumulation in water-stressed ndhb-inactivated tobacco mutants. planta 222: 502-511.
7. munné-bosch s, shikanai t, asada k, 2005. enhanced ferredoxin-dependent cyclic electron flow around photosystem i and -tocopherol quinone accumulation in water-stressed ndhb-inactivated tobacco mutants. planta 222: 502-511.
8. li x-g, bi y-p, zhao s-j, meng q-w, he q-w, zou q, 2005. effects of short-term chilling stress on the photosystems and chloroplast ultrastructure in sweet pepper. agricultural sciences in china 4: 429-435.
9. laisk a, eichelmann h, oja v, rasulov b, padu e, bichele i, pettai h, kull o, 2005. adjustment of leaf photosynthesis to shade in a natural canopy: rate parameters. plant cell and environment 28: 375-388.
10. ivanov b, asada k, kramer dm, edwards g, 2005. characterization of photosynthetic electron transport in bundle sheath cells of maize. i. ascorbate effectively stimulates cyclic electron flow around ps i. planta 220: 572-581.
11. ifuku k, yamamoto y, ono t-a, ishihara s, sato f, 2005. psbp protein, but not psbq protein, is essential for the regulation and stabilization of photosystem ii in higher plants. plant physiology 139: 1175–1184.
12. hirotsu n, makino a, yokota s, mae t, 2005. the photosynthetic properties of rice leaves treated with low temperature and high irradiance plant cell and physiology 46: 1377-1383.
13. gerloff-elias a, spijkerman e, pröschold t, 2005. effect of external ph on the growth, photosynthesis and photosynthetic electron transport of chlamydomonas acidophila negoro, isolated from an extremely acidic lake (ph 2.6). plant cell and environment 28: 1218-1229.
14. georgieva k, maslenkova l, peeva v, markovska y, stefanov d, tuba z, 2005. comparative study on the changes in photosynthetic activity of the homoiochlorophyllous desiccation-tolerant haberlea rhodopensis and desiccation-sensitive spinach leaves during desiccation and rehydration. photosynthesis research 85: 191-203.
15. endo t, kawase d, sato f, 2005. stromal over-reduction by high-light stress as measured by decreases in p700 oxidation by far-red light and its physiological relevance. plant cell and physiology 46: 775-781.
16. egorova ea, drozdova is, bukhov ng, 2005. modulating effect of far-red light on activities of alternative electron transport pathways related to photosystem i russian journal of plant physiology 52: 709-716.
17. dzhibladze tg, polesskaya og, alekhina nd, egorova ea, bukhov ng, 2005. redox states of photosystems i and ii in irradiated leaves of wheat seedlings grown under different conditions of nitrogen nutrition. russian journal of plant physiology 52: 141-146.
18. ducruet j-m, roman m, havaux m, janda t, gallais a, 2005. cyclic electron flow around psi monitored by afterglow luminescence in leaves of maize inbred lines (zea mays l.): correlation with chilling tolerance. planta 221: 567-579.
19. ducruet j-m, roman m, havaux m, janda t, gallais a, 2005. cyclic electron flow around ps i monitored by afterglow luminescence in leaves of maize inbred lines (zea mays l.): correlation with chilling tolerance. planta 221: 567-579.
20. bukhov ng, egorova ea, 2005. nonmonotonic redox conversions of p700 in leaves irradiated with far-red light originate from variable contributions of several alternative electron transport pathways during the induction period russian journal of plant physiology 52: 571-577.
21. bukhov ng, egorova ea, 2005. identification of ferredoxin-dependent cyclic electron transport around photosystem i using the kinetics of dark p700+ reduction. russian journal of plant physiology 52: 283-287.
22. bukhov ng, egorova ea, 2005. evidence for the operation of alternative electron transport routes through photosystem i in intact barley leaves under weak and moderate white light. russian journal of plant physiology 52: 1-6.
23. bukhov ng, dzhibladze tg, egorova ea, 2005. elevated temperatures inhibit ferredoxin-dependent cyclic electron flow around photosystem i russian journal of plant physiology 52: 578-583.
24. zhang s, scheller hv, 2004. photoinhibition of photosystem i at chilling temperature and subsequent recovery in arabidopsis thaliana plant cell and physiology 45: 1595-1602.
25. zhang s, scheller hv, 2004. light-harvesting complex ii binds to several small subunits of photosystem i. the journal of biological chemistry 279: 3180-3187.
26. yabe t, morimoto k, kikuchi s, nishio k, terashima i, nakai m, 2004. the arabidopsis chloroplastic nifu-like protein cnfu, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem i the plant cell 16: 993-1007.
27. stöckel j, oelmüller r, 2004. a novel protein for photosystem i biogenesis. the journal of biological chemistry 279: 10243-10251.
28. schöttler ma, kirchhoff h, weis e, 2004. the role of plastocyanin in the adjustment of the photosynthetic electron transport to the carbon metabolism in tobacco. plant physiology 136: 4265-4274.
29. mackenzie tdb, burns ra, campbell da, 2004. carbon status constrains light acclimation in the cyanobacterium synechococcus elongatus. plant physiology 136: 3301-3312.
30. bukhov ng, govindachary s, rajagopal s, joly d, carpentier r, 2004. enhanced rates of p700+ dark-reduction in leaves of cucumis sativus l. photoinhibited at chilling temperature. planta 218: 852-861.
31. bukhov ng, govindachary s, egorova ea, carpentier r, 2004. recovery of photosystem i and ii activities during re-hydration of lichen hypogymnia physodes thalli. planta 219: 110-120.
32. amann k, lezhneva l, wanner g, herrmann rg, meurer j, 2004. accumulation of photosystem one1, a member of a novel gene family, is required for accumulation of [4fe-4s] culster-containing chloroplast complexes and antenna proteins. the plant cell 16: 3084-3097.
33. 姚正菊, 葉濟(jì)宇, 米華玲, 2003. 高溫脅迫對(duì)煙草葉綠體nadph脫氫酶復(fù)合體活性的促進(jìn). 植物生理與分子生物學(xué)學(xué)報(bào) 29: 395-400.
34. wang h-w, su j-h, shen y-g, 2003. difference in response of photosynthesis to bisulfite between two wheat genotypes. journal of plant physiology and molecular biology 29: 27-32.
35. wang h-w, mi h, ye j-y, deng y, shen y-k, 2003. low concentrations of nahso3 increase cyclic photophosphorylation and photosynthesis in cyanobacterium synechocystis pcc6803. photosynthesis research 75: 151-159.
36. shikanai t, müller-moulé p, munekage y, niyogi kk, pilonc m, 2003. paa1, a p-type atpase of arabidopsis, functions in copper transport in chloroplasts the plant cell 15: 1333-1346.
37. ryu j-y, suh k-h, chung y-h, park y-m, chow ws, park y-i, 2003. cytochrome c oxidase of the cyanobacterium synechocystis sp. pcc 6803 protects photosynthesis from salt stress. molecules and cells 16: 74-77.
38. rajagopala s, egorova ea, bukhov ng, carpentier r, 2003. quenching of excited states of chlorophyll molecules in submembrane fractions of photosystem i by exogenous quinones. biochimica et biophysica acta 1606: 147-152.
39. rajagopal s, carpentier r, 2003. retardation of photo-induced changes in photosystem i submembrane particles by glycinebetaine and sucrose. photosynthesis research 78: 77-85.
40. oja v, eichelmann h, peterson rb, rasulov b, laisk a, 2003. deciphering the 820 nm signal: redox state of donor side and quantum yield of photosystem i in leaves. photosynthesis research 78: 1-15.
41. maiwald d, dietzmann a, jahns p, pesaresi p, joliot p, joliot a, levin jz, salamini f, leister d, 2003. knock-out of the genes coding for the rieske protein and the atp-synthase δ-subunit of arabidopsis. effects on photosynthesis, thylakoid protein composition, and nuclear chloroplast gene expression. plant physiology 133: 191-220.
42. krause gh, grube e, virgo a, winter k, 2003. sudden exposure to solar uv-b radiation reduces net co2 uptake and photosystem i efficiency in shade-acclimated tropical tree seedlings. plant physiology 131: 745-752.
43. hanson dt, franklin la, samuelsson g, badger mr, 2003. the chlamydomonas reinhardtii cia3 mutant lacking a thylakoid lumen-localized carbonic anhydrase is limited by co2 supply to rubisco and not photosystem ii function in vivo. plant physiology 132: 2267-2275.
44. golding aj, johnson gn, 2003. down-regulation of linear and activation of cyclic electron transport during drought. planta 218: 107-114.
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澤泉科技有限公司是一家致力于引進(jìn)歐美高科技產(chǎn)品并提供系統(tǒng)解決方案的高科技公司。澤泉科技不僅向用戶提供儀器設(shè)備的選型、采購(gòu)、安裝調(diào)試、技術(shù)培訓(xùn)和售后支持等服務(wù)，還向用戶提供系統(tǒng)解決方案及集成服務(wù)。 公司由陸地環(huán)境部、水環(huán)境部和系統(tǒng)集成部組成。陸地環(huán)境部主要提供植物、土壤、氣象和環(huán)境領(lǐng)域需要的儀器，并提供氣象監(jiān)測(cè)、節(jié)水灌溉、生態(tài)監(jiān)測(cè)站等項(xiàng)目的系統(tǒng)解決方案；水環(huán)境部主要提供海洋和淡水研究中需要的儀器，并提供近岸、水上（浮標(biāo)型）和移動(dòng)型（水下機(jī)器人）監(jiān)測(cè)/預(yù)警系統(tǒng)的系統(tǒng)解決方案；系統(tǒng)集成部主要為專業(yè)用戶提供定制個(gè)性化服務(wù)。
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