【文獻(xiàn)標(biāo)題】Rapeseed protein-derived ACE inhibitory peptides LY, RALP ａnd GHS show
antioxidant ａnd anti-inflammatory effects on spontaneously hypertensive rats
【作者】Rong He，Yujiao Wang，Yijie Yang，et.al
【作者單位】南京財(cái)經(jīng)大學(xué)（Nanjing University of Finance ａnd Economics）
【文獻(xiàn)中引用產(chǎn)品】
人白細(xì)胞介素6(IL-6)ELISA試劑盒
人腫瘤壞死因子α(TNF-α)ELISA試劑盒
人誘導(dǎo)型一氧化氮合成酶(iNOS)ELISA試劑盒
【關(guān)鍵詞】Rapeseed peptide，ACE inhibition，Anti-inflammatory effect，Cytokine，Oxidative stress
【DOI】https://doi.org/10.1016/j.jff.2019.02.031
【影響因子(IF)】5.9
【出版期刊】《Journal of Functional Foods》
【產(chǎn)品原文引用】
Materials ａnd reagents
RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, ａnd hematoxylin ａnd eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit ａnd Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit ａnd Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science ａnd Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) ａnd Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).