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Product Name | HLA-G Antibody (Center) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | WBIHCFC |
WB~~1:100~500 IHC~~1:10~50 FC~~1:10~50: | |
Species Reactivity | Human |
Concentration | 1 mg/ml |
Purification |
Gene Synonyms | |
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Alternative Names | More |
Molecular Weight(MW) | 38224 Da |
Function | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells |
Tissue Specificity | Expressed in trophoblasts. |
Cellular Localization | Membrane; Single-pass type I membrane protein |
Entrez Gene | 3135 |
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Western blot analysis of HLA-G Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.
Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HLA-G Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:100~500 IHC~~1:10~50 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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