標(biāo)題MCM2 Antibody Catalog Number：OM284353

提供者：上海篤瑪生物科技有限公司發(fā)布時(shí)間：2024/9/26 閱讀次數(shù)：38次 >>進(jìn)入該公司展臺(tái)

Product Name	MCM2 Antibody
Antibody Type	Primary Antibodies
Product description	MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, ａnd 7, ａnd has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, ａnd thus regulated by, protein kinases CDC2 ａnd CDC7.1) Saade, E., et al. Proteomics 9(21):4934-4943(2009) More
Immunogen	This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity	HumanMouse
Concentration	1 mg/ml
Purification	Affinity purified

Gene Symbol	MCM2
Alternative Names	DNA replication licensing factor MCM2 Minichromosome maintenance protein 2 homolog Nuclear protein BM28 MCM2 BM28 CCNL1 CDCL1 KIAA0030 More
Molecular Weight(MW)	102 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Xenopus

Entrez Gene	4171
Protein Accession	P49736

Application

MCM2 Antibody immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.
Application

Western blot analysis of MCM2 Antibody in HL-60 cell line lysates (35ug/lane)
Application

Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Form	Liquid
Storage Instructions	Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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