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| Product Name | PHB Antibody (C-term E194) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Alternative Names | |
|---|---|
| Molecular Weight(MW) | 29804 Da |
| Function | Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity ａnd in aging |
| Tissue Specificity | Widely expressed in different tissues. |
| Cellular Localization | Mitochondrion inner membrane (By similarity) |
| Entrez Gene | 5245 |
|---|
Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB gene (Lane 2) (Origene Technologies).
Western blot analysis of anti-PHB Antibody (C-term) in Jurkat cell line lysates (35ug/lane).PHB (arrow) was detected using the purified Pab.
Formalin-fixed ａnd paraffin-embedded human breast carcinoma reacted with PHB Antibody (C-term E194), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of NCI-H292 cells using PHB Antibody (C-term E194)(bottom histogram) compared to a negative control cell ( histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Application Notes | WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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