標(biāo)題Bmi1 [B3-G5] Catalog Number：OM628508

提供者：上海篤瑪生物科技有限公司發(fā)布時(shí)間：2024/9/20 閱讀次數(shù)：44次 >>進(jìn)入該公司展臺

Product Name	Bmi1 [B3-G5]
Antibody Type	Primary Antibodies
Product description	The Bmi-1 was identified initially as an oncogene that cooperates with c-myc in the generation of B-cell lymphoma. It contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, ａnd oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, ａnd cell-cycle arrest. More
Immunogen	Recombinant protein within human Bmi1 full sequence.

Alternative Names	B lymphoma Mo MLV insertion region (mouse) antibody B lymphoma Mo MLV insertion region 1 homolog antibody Bmi 1 antibody BMI1 antibody BMI1 polycomb ring finger oncogene antibody BMI1_HUMAN antibody Flvi 2/bmi 1 antibody FLVI2/BMI1 antibody MGC12685 antibody Murine leukemia viral (bmi 1) oncogene homolog antibody Oncogene BMI 1 antibody PCGF 4 antibody PCGF4 antibody Polycomb complex protein BMI 1 antibody Polycomb complex protein BMI-1 antibody Polycomb group protein Bmi1 antibody Polycomb group ring finger 4 antibody Polycomb group RING finger protein 4 antibody RING finger protein 51 antibody RNF 51 antibody RNF51 antibody More
Molecular Weight(MW)	37kDa
Cellular Localization	Nucleus, Cylasm

SwissProt ID	P35226 P25916

Application

Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.Positive control:Lane 1: 293T Lane 2: JurkatLane 3: Hela Lane 4: MCF-7Lane 5: HepG2 Lane 6: NIH/3T3Lane 7: PC12 Lane 8: Mouse kidneyLane 9: Human kidney Lane 10: K562Lane 11: Human brain
Application

Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig4: ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Application

Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Application

Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
Application

Fig8: Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

Positive Control	Hela, HepG2, Jurkat, 293T, MCF-7, PC12, K562, NIH/3T3, A549, LOVO, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, mouse kidney tissue, human kidney tissue, human brain tissue.
Application Notes	WB:1:1,000 IHC:1:200 ICC:1:200 FC:1:100-1:200 More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

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