標(biāo)題NF-L antibody Catalog Number：OM265293

提供者：上海篤瑪生物科技有限公司發(fā)布時間：2024/9/20 閱讀次數(shù)：25次 >>進(jìn)入該公司展臺

Product Name	NF-L antibody
Antibody Type	Primary Antibodies
Product description	Mouse Monoclonal antibody [1D2] to NF-LAlpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M ａnd NF-H, having similar protein sequence motifs ａnd a similar intron organization. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M ａnd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study ａnd classify neuronal types ａnd follow their processes in sections ａnd in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early ａnd convenient diagnostic feature of neuronal progenitors cells ａnd other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. More
Immunogen	Recombinant full length protein (Rat) expressed in ａnd purified from E. coli.

Clonality	Monoclonal
Isotype	IgG
Clone Number	1D2
Host Species	Mouse
Tested Applications	ICC/IFIHC-FIHC-PWB
	IF: Use at a dilution of 1/500. IHC-Fr: Use at a dilution of 1/5000 (formalin fixed, ABC method). IHC-P: Use at a dilution of 1/5000 (ABC method). WB: Use at a dilution of 1/10000. Detects a band of approximately 66 kDa. Optimal dilutions/concentrations should be determined by the end user.: More
Species Reactivity	HumanRatMammalian species
Concentration	1 mg/ml

Gene Synonyms	Nefml Neflmapped Nefl_mapped 68kDa Neurofilament 24589 Nefl More
Target	NF-L

Application

Mixed cultures of rat CNS cells stained with (red) ａnd GeneTex chicken antibody to Microtubule associated protein 2 (MAP2- green, using antibody ). Thea-internexin is localized primarily in neuronal axons in these cultures, while the perikarya ａnd dendrites of neurons stain strongly for MAP2.
Application

Left three lanes show Coomassie brilliant blue stained extracts of rat brain stem, cat cerebral cortex ａnd human cerebral cortex (R, C ａnd H respectively). Right three lanes are corresponding blots reacted with stains a band of about 66kDa in all three preparations.

Application Notes	IF: Use at a dilution of 1/500. IHC-Fr: Use at a dilution of 1/5000 (formalin fixed, ABC method). IHC-P: Use at a dilution of 1/5000 (ABC method). WB: Use at a dilution of 1/10000. Detects a band of approximately 66 kDa. Optimal dilutions/concentrations should be determined by the end user.: More

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Preservative: 5mM Sodium Azide; Constituents: Tissue Culture Supernatant

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