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| Product Name | Nrf2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The NF-E2 DNA binding protein is composed of two subunits, p45 ａnd MafK. It regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (Mares). A family of NF-E2- related proteins, which are collectively known as the Cap ‘n’ collar (CNC) family ａnd include Nrf1 (also designated TCF11), Nrf2 ａnd Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind Mare sequences. The Nrf proteins also bind the antioxidant response element (ARE) ａnd are implicated in the regulation of detoxification enzymes ａnd the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B ａnd Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1. Nrf2 is widely expressed ａnd is thought to translocate to the nucleus after treatment with xenobiotics ａnd antioxidants, which stimulate its release from its repressor protein, Keap1. The gene encoding human Nrf3 maps to chromosome 7p15.2. Nrf3 is highly expressed in placenta, B cells ａnd monocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Nrf2 of mouse, rat ａnd human origin by WB, IP, IF, IHC(P) ａnd ELISA; also reactive with additional species, including canine, bovine ａnd porcine: | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | |
|---|---|
| Tissue Specificity | epie mapping at the C-terminus of Nrf2 of human origin |
| Entrez Gene | 4780 |
|---|
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Western blot analysis of Nrf2 expression in non-transfected 293T: (A), mouse Nrf2 transfected 293T: (B) ａnd Jurkat (C) whole cell lysates.
Western blot analysis of Nrf2 expression in THP-1 nuclear extract.
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse kidney tissue showing nuclear localization.
| Application Notes | recommended for detection of Nrf2 of mouse, rat ａnd human origin by WB, IP, IF, IHC(P) ａnd ELISA; also reactive with additional species, including canine, bovine ａnd porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol |
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