The Sf-RVN^™ Insect Cell Line is a rhabdovirus-negative Sf9 cell line that can be grown in both adherent ａnd suspension culture. Sf-RVN^™ Insect Cell Line has been adapted for growth in chemically defined medium to be used for the production of recombinant proteins, adeno-associated viruses (AAV) ａnd viral-like particles (VLP). The cell line was tested for adventitious agents ａnd banked under GMP conditions. A technical user guide, with detailed protocols for optimal performances, is provided with the cell line.The EX-CELL^® CD Insect Cell Medium, recommended for use, is optimized to get excellent growth ａnd productivity of the Sf-RVN^™ Insect Cell Line. Combined, these two products form the Sf-RVN^™ Platform.

Cell Line Origin

Sf-RVN^™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith ａnd C. Cherry. Sf-RVN^™ was isolated from Sf9 by GlycoBac, LLC.

Sf-RVN^™ cells are provided to customers in vials containing 1 mL at 10 x 10⁶cells/mL. Cells are banked in EX-CELL^® CD Insect Cell Medium ａnd 10% DMSO

Sf-RVN^™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith ａnd C. Cherry. Sf-RVN ^™ was isolated from Sf9 by GlycoBac, LLC.

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

EXACT-AIR is a registered trademark of Merck KGaA, Darmstadt, Germany

Sf-RVN is a trademark of Merck KGaA, Darmstadt, Germany