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別名:HE Agar, HEA, Hektoen Enteric Agar
屬性
Agency
FDA-BAM
according to ISO 21567
according to ISO 6579
質(zhì)量水平
200
sterilization compatibility
autoclavable compatible
形式
granular (dehydrated culture medium)
包裝
pkg of 500 g
manufacturer/tradename
GranuCult® prime
儲存條件
protect from light (Keep dry and tightly closed)
technique(s)
microbiological culture: suitable
顏色
dark green
pH值(酸堿度)
7.5-7.9 (25 °C, 75 g/L in H2O)
溶解性
75 g/L
application(s)
clinical
food and beverages
industrial qc
pharmaceutical
microbiology
儲存溫度
15-25°C
適用性
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
說明
一般描述
GranuCult® prime Hektoen Enteric Agar provides good colonial differentiation and inhibits some coliforms and other non-lactose-fermenting bacteria, thereby facilitating the isolation and differentiation of Salmonella spp. and Shigella spp. from samples such as food chain and other materials.Lactose-positive colonies have a different color from lactose-negative ones due to the presence of the two indicators for carbohydrate dissimilation: bromothymol blue and acidic fuchsin. The two additional carbohydrates, sucrose (saccharose) and salicin, also cause the color difference in colonies. The black color in the H2S-positive colonies is due to thiosulfate as a reactive compound with an iron salt as an indicator.This culture medium is moderately to highly selective. The mixture of bile salts suppresses the growth of most of the accompanying microorganisms. The inhibitory effect of bile salts on the growth of Shigella spp. is reduced by relatively large amounts of peptone and carbohydrates, which allow good growth. Agar is the solidifying agent.
應(yīng)用
GranuCult® prime Hektoen Enteric Agar is suitable for the isolation and differentiation ofSalmonella and Shigella from the food chain and other materials.
特點和優(yōu)勢
GranuCult® offers superior granulated culture media.
Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
Excellent wettability, solubility, and free-flowing properties
Convenient, with minimal component separation and clumping, even under warm or humid conditions.
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Granulation technology allows many supplements to be included, with no need to add these separately
儲存及穩(wěn)定性
Self-prepared plates can be stored in the dark and protected against evaporation:
acc. to ISO 21567 at (5 ± 3 °C) for up to 2 weeks
acc. to FDA-BAM at (4 ± 2 °C) for up to 30 days
acc to Corry et al., (2012) at (5 ± 3 °C) for up to 3 weeks
分析說明
Salmonella spp. produce transparent green to blue-green colonies, with or without black centers, or may appear completely black due to H2S production.
Shigella spp. produce green, transparent moist colonies.
Lactose, Sucrose or Salicin fermenting gram-negative bacteria may be inhibited or produce red to salmon colored colonies with or without precipitation zone.
其他說明
Do not use clumped or discolored medium.
The prepared medium is clear to slightly opalescent and blue-green to blue-grey.
Before inoculation, allow the prepared medium to equilibrate at room temperature if it is stored at a lower temperature.
There should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture, following the procedure as described by EN ISO 11133.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
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