Mouse Anti-BrdU monoclonal antibody
（Bromodeoxyuridine）
Cat. Number: Rsm-0917M
Quantity size: 0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: Bromodeoxyuridine （BrdU） is a thymidine analog ａnd is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling ａnd detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, ａnd the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood （Campana et al., 1988）, tissues （Schutte et al., 1987; Hayashi, et al., 1988）, tumors （Hoshino et al., 1986; Morstyn et al., 1983）, as well as for determining plasma cell labeling indices （Greipp et al., 1985）.
Specificity: · anti-BrdU is a mouse monoclonal antibody unconjugated
· use for elisa, immunoprecipitation ａnd immunohistochemistry
·affinity chromatography purification, purity :>95%
· Isotype: IgG
Mouse Anti-BrdU monoclonal antibody
Application: · Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
