試劑盒名稱：微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒

規(guī) 格：96T

產(chǎn)品的用途：僅供科研究使用！

價(jià)格：電議,或咨詢?cè)诰�客服QQ,或者以郵件形式發(fā)到我司郵箱，齊一生物科技（上海）有限公司提供大量品牌進(jìn)口原裝，分裝，以及穩(wěn)定性強(qiáng)的國(guó)產(chǎn)ELISA試劑盒，性價(jià)比極高。歡迎您前來(lái)選購(gòu)！

本試劑盒主要用于各種動(dòng)物肌肉組織、肝臟、尿液、血清、飼料等中 殘留定量檢測(cè)！

微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒試驗(yàn)原理：
該產(chǎn)品是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知待測(cè)物質(zhì)濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將待測(cè)物質(zhì)和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后，加入親和素標(biāo)記過(guò)的HRP。再經(jīng)過(guò)溫育和洗滌，去除未結(jié)合的酶結(jié)合物，然后加入底物A、B，和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中待測(cè)物質(zhì)的濃度呈比例關(guān)系。

試劑盒內(nèi)容及其配制

試劑盒自備材料
1）蒸餾水。
2）加樣器：5ul、10ul、50ul、100ul、200ul、500ul、1000ul。
3）振蕩器及磁力攪拌器等。

檢測(cè)試劑盒安全性
1）避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。
2）實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3）不要用嘴吸取試劑盒里的任何成份

操作注意事項(xiàng)
1）試劑應(yīng)按標(biāo)簽說(shuō)明書儲(chǔ)存，使用前恢復(fù)到室溫。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。
2）實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。
3）不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用。保質(zhì)前使用。
4）使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。
5）使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6）洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。
7）底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。
8）加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。
9）按照說(shuō)明書中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

樣品收集、處理及保存方法
1）血清－－－－-操作過(guò)程中避免任何細(xì)胞刺激。使用不含熱原和內(nèi)毒素的試管。收集血液后，1000×g離心10分鐘將血清和紅細(xì)胞迅速小心地分離。
2）血漿－－－－-EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè)。1000×g離心30分鐘去除顆粒。
3）細(xì)胞上清液－－-1000×g離心10分鐘去除顆粒和聚合物。
4）組織勻漿－－－－-將組織加入適量生理鹽水搗碎。1000×g離心10分鐘，取上清液
5）保存－－－－－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒】

試劑盒試劑的準(zhǔn)備
1）標(biāo)準(zhǔn)品：標(biāo)準(zhǔn)品的系列稀釋應(yīng)在實(shí)驗(yàn)時(shí)準(zhǔn)備，不能儲(chǔ)存。稀釋前將標(biāo)準(zhǔn)品振蕩混勻。
2）洗滌緩沖液（50×）的稀釋：蒸餾水50倍稀釋。

檢測(cè)試劑盒操作步驟
1）使用前，將所有試劑充分混勻。不要使液體產(chǎn)生大量的泡沫，以免加樣時(shí)加入大量的氣泡，產(chǎn)生加樣上的誤差。
2）根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù)。每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔。每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定，能使用復(fù)孔的盡量做復(fù)孔。標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi)。
3）加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi)。立即加入50ul的生物素標(biāo)記的抗體。蓋上膜板，輕輕振蕩混勻，37℃溫育1小時(shí)。
4）甩去孔內(nèi)液體，每孔加滿洗滌液，振蕩30秒，甩去洗滌液，用吸水紙拍干。重復(fù)此操作3次。如果用洗板機(jī)洗滌，洗滌次數(shù)增加一次。
5）每孔加入80ul的親和鏈酶素-HRP，輕輕振蕩混勻，37℃溫育30分鐘。
6）甩去孔內(nèi)液體，每孔加滿洗滌液，振蕩30秒，甩去洗滌液，用吸水紙拍干。重復(fù)此操作3次。如果用洗板機(jī)洗滌，洗滌次數(shù)增加一次。
7）每孔加入底物A、B各50ul，輕輕振蕩混勻，37℃溫育10分鐘。避免光照。
8）取出酶標(biāo)板，迅速加入50ul終止液，加入終止液后應(yīng)立即測(cè)定結(jié)果。
9）在450nm波長(zhǎng)處測(cè)定各孔的OD值。

檢測(cè)試劑盒局限
6號(hào)標(biāo)準(zhǔn)品以上的結(jié)果為非線性的，根據(jù)此標(biāo)準(zhǔn)曲線無(wú)法得到精確的結(jié)果。

微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒性能
1. 靈敏度：*小的檢測(cè)濃度小于1號(hào)標(biāo)準(zhǔn)品。稀釋度的線性。樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.990。
2. 特異性：不與其它細(xì)胞因子反應(yīng)。
3. 重復(fù)性：板內(nèi)、板間變異系數(shù)均小于10%。

檢測(cè)試劑盒結(jié)果判斷與分析
1、儀器值：于波長(zhǎng)450nm的酶標(biāo)儀上讀取各孔的OD值
2、以吸光度OD值為縱坐標(biāo)（Y），相應(yīng)的待測(cè)物質(zhì)標(biāo)準(zhǔn)品濃度為橫坐標(biāo)（X），做得相應(yīng)的曲線，樣品的待測(cè)物質(zhì)含量可根據(jù)其OD值由標(biāo)準(zhǔn)曲線換算出相應(yīng)的濃度。

 齊一生物科技有限公司主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒，種類齊全，價(jià)格實(shí)惠，購(gòu)買我公司產(chǎn)品的新老客戶，可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù)，可檢測(cè)人源，小鼠，大鼠，豬，狗，猴，兔，羊，雞，牛，鴨子，魚，植物等各種樣本。全程提供技術(shù)支持。如有什么問(wèn)題請(qǐng)給我們電話或者留言，告訴我您的需要，并留下您寶貴的意見和建議。

9240984  PCB, p&c, cooling device, Qsym         CNY                           

9240983  Cooling device, eluate drawer, Qsym           CNY                           

9240952  Handler, Qsym              CNY                           

9240950  PCB, xyz, portal, Qsym        CNY                           

9240836  QIAcube, Full Agreement, no PM  "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"      CNY                           

9240684  Pogo Stick Kit, AP196/98             CNY                           

9240802  Performance, IQ/OQ, EZ1           CNY                           

9240782  Update, Weigh Cell, AP196           CNY                           

9240779  500 ml Reagent #6 Update, AP98         CNY                           

9240770  Assy, Capper Shuttle, AP96          CNY                           

9240767  8 OUT Relay, F2-08TRS, AP96           CNY                           

9240765  32 IN Module, D2-32ND3, AP96         CNY                           

9241778  RGQ, Inspection Service       "The Annual Inspection Service, Rotorgene-Q is a Regional Repair Center equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the Rotorgene are inspected ａnd tested ａnd an OTV check is conducted. Performed tests ａnd test results are documented in a GMP/GLP-compliant Report."     CNY                           

9241730  BR Universal ApplicationPack Veterinary             CNY                           

9241394  C-Nut set, EZ1a            CNY                            【微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒】

9240749  Belt, Shaker, AP            CNY                           

9243644  Guided Valid Support, QuantiPlex/OPHyres  The QIAGEN Quantiplex/Quantiplex HYres Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the Quantiplex/Quantiplex HYres chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on operation to match the lab’s forensic workflow. The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  CNY                           

9243642  Pyro Q24, Repair Fee    "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243540  LiquiChip, PM Service   "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243114  Flow Control Valve, Weigh Cell, AP             CNY                           

9242862  QIArent&go - Rent out fee QSSP Cabinet           CNY                           

9242676  QS RGQ, Installation     The Installation, QIAsymphony RGQ facilitates the quick implementation of the QIAsymphony RGQ into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of QIAsymphony RGQ instruments ａnd provides the user with an introductory training. The installation includes or labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.     CNY                           

5000-1324      Motor 230V, SKR1        CNY                           

206045    Type-it Fast SNP Probe PCR Kit (800) "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"  CNY                           

9001911  PyroMark Q24 MDx, Priority       PyroMark Q24 MDx, Priority includes the Pyromark Q24 MDx system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. (In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)   CNY                           

965842    QIAamp 96 DNA Swab BioRobot Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps   CNY                           

133186    NeXtalStock Citric acid pH 4.0 (200)          CNY                           

133185    NeXtalStock Citric acid pH 3.8 (200)          CNY                           

9018554  Cover, front, left, EZ1a         CNY                           

9018529  Communicator, Software CD, EZ1a            CNY                           

51206     FlexiGene DNA Kit (250)      For purification of DNA from 250 ml whole blood: Buffers, QIAGEN Protease CNY                            【微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒】

51106      QIAamp DNA Blood Mini Kit (250)      For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)  CNY                           

50214     QIAamp UCP Pathogen Mini Kit (50),G              CNY                           

DQYC26 農(nóng)殘速測(cè)試劑（行標(biāo)）       農(nóng)殘速測(cè)試劑（行標(biāo)）                                  

DQYC27 農(nóng)殘速測(cè)試劑       農(nóng)殘速測(cè)試劑                                  

DQYC28 農(nóng)殘速測(cè)試劑（國(guó)標(biāo)）       農(nóng)殘速測(cè)試劑（國(guó)標(biāo)）                                  

DQYC29 "農(nóng)殘速測(cè)增強(qiáng)劑

"      "農(nóng)殘速測(cè)增強(qiáng)劑

"                                 

DQYQYC30   農(nóng)藥殘留速測(cè)卡 農(nóng)藥殘留速測(cè)卡                              

RT089     "DNA提取試劑盒

"      100次/盒                                   【微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒】

RT090     轉(zhuǎn)基因玉米成分PAT檢測(cè)試劑盒       100次/盒                                  

RT091     "轉(zhuǎn)基因大豆成分CP4-EPSPS檢測(cè)試劑盒

"      100次/盒                                  

QYDC31 甲醛快速檢測(cè)試劑盒   100次/盒                                  

QYDC32 吊白塊快速檢測(cè)試劑盒      100次/盒                                  

QYDC33 二氧化硫快速檢測(cè)試劑盒   100次/盒                                  

QYDC34 亞硝酸鹽快速檢測(cè)試劑盒   100次/盒                                  

QYDC35 硼砂快速檢測(cè)試劑盒   100次/盒                                  

QYDC36 雙氧水快速檢測(cè)試劑盒      100次/盒                                  

QYDC37 工業(yè)堿快速檢測(cè)試劑盒       100次/盒                                  

QYDC38 硝酸鹽快速檢測(cè)試劑盒      50次/盒                             

QYDC39 甲醇檢測(cè)試劑盒    50次/盒                             

QYDC40 奶粉蛋白質(zhì)含量速測(cè)盒       50次/盒                             

QYDC41 液態(tài)奶新鮮度速測(cè)盒    50次/盒                             

QYDC42 過(guò)氧化苯甲酰速測(cè)盒    50次/盒                             

QYDC43 面粉中鋁快速檢測(cè)試劑盒    50次/盒                             

QYDC44 孔雀石綠篩查柱檢測(cè)試劑盒       10次/盒                              【微囊藻毒素/節(jié)球藻毒素(DM)檢測(cè)試劑盒】

QYDC45 孔雀石綠組織檢測(cè)試劑盒    10次/盒                             

QYDC46 蘇丹紅快速檢測(cè)試劑盒      50次/盒                             

QYDC47 水溶性非食用色素速測(cè)盒    50次/盒                             

QYDC48 食用色素速測(cè)盒    50次/盒                             

QYDC49 肉類水份檢測(cè)卡    60次/盒                             

DQYC50 糖精鈉速測(cè)盒       50次/盒                             

DQYC51 肉類新鮮度速測(cè)盒       50次/盒                             

DQYC52 食醋中游離礦酸速測(cè)試紙    40片/盒                             

DQYC53 食醋中總酸快速測(cè)定試液    25次/盒                             

DQYC54 醬油總酸氨基酸態(tài)氮速測(cè)液       20份/瓶                             

DQYC55 假冒偽劣味精速測(cè)試液包   20次/盒                             

QY173    嘔吐毒素檢測(cè)試劑盒    96孔/盒 糧食作物（玉米、大米、豆類、花生、麥類等）、飼料等                         

QY174    微囊藻毒檢測(cè)試劑盒    96孔/盒 適用于水樣、藍(lán)藻、魚肉等樣品                           

DQYC20 蔬菜農(nóng)藥速測(cè)卡    蔬菜農(nóng)藥速測(cè)卡                               

DQYC21 茶葉農(nóng)藥速測(cè)卡    茶葉農(nóng)藥速測(cè)卡                               

DQYC22 "糧食農(nóng)藥速測(cè)卡

"      "糧食農(nóng)藥速測(cè)卡

"                                 

DQYC23 蔬菜農(nóng)藥檢測(cè)卡    蔬菜農(nóng)藥檢測(cè)卡                               

DQYC24 高靈敏度農(nóng)藥速測(cè)卡    高靈敏度農(nóng)藥速測(cè)卡                               

DQYC25 農(nóng)殘速測(cè)試劑       農(nóng)殘速測(cè)試劑