價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質產(chǎn)品。

【大腸桿菌O157試紙條】注意事項

1. 當混合蛋白溶液時應盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標準曲線，做復孔。

5. 如標本中待測物質含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標準品、檢測溶液工作液時，請以相應的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【大腸桿菌O157試紙條】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【大腸桿菌O157試紙條】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【大腸桿菌O157試紙條】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

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QKT0593     ａ-L-巖藻糖苷酶AFU檢測試劑盒CNPF法                                    

QKT0594     甘油三酯TG檢測試劑盒酶比色法                                      

QKT0595     低密度脂蛋白膽固醇LDL檢測試劑盒酶直接法                                      【大腸桿菌O157試紙條】

QKT0596     載脂蛋白aLpa檢測試劑盒比濁法                                       

QKT0597     抗環(huán)瓜氨酸肽抗體測定試劑盒膠乳增強免疫比濁法                                     

QKT0598     脂肪酶測定試劑盒LPS  酶比色法                                

QKT0599     β-羥丁酸測定試劑盒β-HB 酶法                                 

QKT0600     二氧化碳測定試劑盒CO2    酶法                                 

QKT0601     鋅測定試劑盒Zn      比色法銅測定試劑盒                                 

QKT0602     缺血修飾白蛋白測定試劑盒IMA       白蛋白-鈷結合試驗法                                

QKT0603     C反應蛋白測定試劑盒CRP 膠乳增強免疫比濁法                                 

QKT0604     鐵測定試劑盒Fe      比色法                             

QKT0605     轉鐵蛋白測定試劑盒TRF     免疫比濁法                            

QKT0606     唾液酸測定試劑盒SA    酶法                                 

QKT0607     胃蛋白酶原Ⅱ測定試劑盒膠乳增強免疫比濁法PGⅡ 膠乳增強免疫比濁法                                 

QKT0608     胃蛋白酶原Ⅰ測定試劑盒膠乳增強免疫比濁法PGⅠ 膠乳增強免疫比濁法                                 

QKT0609     中性粒細胞明膠酶相關脂質運載蛋白測定試劑盒NGAL  膠乳增強免疫比濁法                                 

QKT0610     游離脂肪酸測定試劑盒NEFA     酶法                                 

QKT0611     N-乙酰-β-D-氨基葡萄糖苷酶測定試劑盒NAG   連續(xù)監(jiān)測法                             【大腸桿菌O157試紙條】

QKT0612     肌紅蛋白測定試劑盒膠乳增強免疫比濁法Mb                                        

QKT0613     單胺氧化酶測定試劑盒MAO      連續(xù)監(jiān)測法                            

QKT0614     亮氨酸氨基肽酶測定試劑盒LAP       連續(xù)監(jiān)測法                            

QKT0615     胰島素測定試劑盒膠乳增強免疫比濁法INS                                    

QKT0616     心型脂肪酸結合蛋白測定試劑盒膠乳增強免疫比濁法H-FABP                                       

QKT0617     糖化白蛋白測定試劑盒酶法GA  酶法                                 

QKT0618     纖維蛋白原測定試劑盒FIB  免疫比濁法                            

QKT0619     鐵蛋白測定試劑盒FER  膠乳增強免疫比濁法                                 

QKT0620     纖維蛋白原降解產(chǎn)物測定試劑盒FDP      膠乳增強免疫比濁法                                 

QKT0621     肌鈣蛋白I測定試劑盒cTnI  膠乳增強免疫比濁法                                 

QKT0622     甘膽酸測定試劑盒CG    膠乳增強免疫比濁法                                 

QKT0623     血氨測定試劑盒AMM   酶法                                 

QKT0624     類風濕因子測定試劑盒RF免疫比濁法                                     

QKT0625     抗鏈球菌溶血素O測定試劑盒ASO 免疫比濁法