1.購買我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開始之前，清潔工作臺。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時(shí)儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請?jiān)炷Ｈ〔暮�，將�?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項(xiàng)請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

215011     FastLane Cell cDNA Kit (50)               CNY                             

211822     Certal CHO Detection Kit (100)                 CNY                             

69534       mericon DNA Bacteria Plus Kit (50)                   CNY                             

69504       DNeasy Blood & Tissue Kit (50)        50 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)  CNY                             

9012770  Liquid Handling Kit Plato 7381/7181                  CNY                              【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】

9012746  Syringe pump outlet tubing (5.7 m)                   CNY                             

9010472  LabHand for gel card + microplates                   CNY                             

979013     Fungi ITS2 Primer 1 for Sequencing          CNY                             

59934       EpiTect MethyLight Assay Hs-CDKN2A (100)   "10-fold primer TaqMan probe mix, for real-time MethyLight PCR

For TaqMan probe-based real-time quantification of methylated or unmethylated CpG sites"      CNY                             

59933       #EpiTect MethyLight Assay Hs-ERBB2 (100)    "10-fold primer TaqMan probe mix, for real-time MethyLight PCR

For TaqMan probe-based real-time quantification of methylated or unmethylated CpG sites"      CNY                             

310207     BioMag Goat Anti-Rabbit IgG (500ml)    BioMag goat anti-rabbit IgG secondary antibody suspension (1 mg/ml)         CNY                             

9236430  Motor, gripper replacement kit, TWI                CNY                             

9239106  Grub screw set, Kloehn syringe lock (10)          CNY                             

9239017  Green LSR v2.0 (Coherent)                CNY                             

9239015  Barb Fitting, RCS1             CNY                             

9232697  Vacuum pump, type 84, 100-V                  CNY                             

9238972  Target Prep, Basic Agreement         The Basic Cover Agreement TargetPrep provides full service coverage for the BioRobot TargetPrep. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238786  Angle rotor 100 x 1.5 / 2.2 ml, incl. al               CNY                             

9016839  Locking mechanism, RoboVac base v4.2          CNY                             

9232554  Back Reagent Rack 1.03           CNY                             

9232343  Gasket, auto vac, Base BR8000, v 1.0              CNY                              【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】

9238769  Swing-out rotor 48 x 15 ml complete                CNY                             

9238921  Tooth wheel, BS15            CNY                             

9238912  Power supply 200W, BS96                 CNY                             

9232324  Cable, PS24 to XADAPT board, BR8000            CNY                             

9232205  SplashingCover, Washstation, P3xxx                  CNY                             

9238702  Imbalance switch, magnetic             CNY                             

9231778  Rack for reagents,Trough (for 3 troughs)                   CNY                             

9238691  Relay 2U 24 V           CNY                             

9231752  Bracket, PCON board, auto vac, BR8000                  CNY                             

9231013  Distributor, 1 to 4              CNY                             

9231010  Y-Flat band cable Ground (Plato 7)           CNY                             

9020074  Right Rocker Arm, preassemblied, TLyser                 CNY                             

         肺大靜脈平滑肌細(xì)胞     5 × 105         1ml                             

         肺動(dòng)脈成纖維細(xì)胞         5 × 105         1ml                             

         肺泡上皮細(xì)胞         5 × 105         1ml                             

         氣管上皮細(xì)胞         5 × 105         1ml                             

         支氣管上皮細(xì)胞     5 × 105         1ml                             

         小氣道上皮細(xì)胞     5 × 105         1ml                              【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】

         肺成纖維細(xì)胞         5 × 105         1ml                             

         支氣管平滑肌細(xì)胞         5 × 105         1ml                             

         小氣道平滑肌細(xì)胞         5 × 105         1ml                             

         氣管平滑肌細(xì)胞     5 × 105         1ml                             

         支氣管成纖維細(xì)胞         5 × 105         1ml                             

         心房肌細(xì)胞     5 × 105         1ml                             

         心室肌細(xì)胞     5 × 105         1ml                             

         心肌成纖維細(xì)胞     5 × 105         1ml                             

         心臟微血管內(nèi)皮細(xì)胞     5 × 105         1ml                             

         主動(dòng)脈內(nèi)皮細(xì)胞     5 × 105         1ml                             

         主動(dòng)脈平滑肌細(xì)胞         5 × 105         1ml                             

         臍靜脈內(nèi)皮細(xì)胞     5 × 105         1ml                             

         臍動(dòng)脈內(nèi)皮細(xì)胞     5 × 105         1ml                             

         臍靜脈平滑肌細(xì)胞         5 × 105         1ml                             

         臍動(dòng)脈平滑肌細(xì)胞         5 × 105         1ml                             

         大隱靜脈平滑肌細(xì)胞     5 × 105         1ml                              【人基質(zhì)裂解素(ST2)ELISA試劑盒廠家】

         冠狀動(dòng)脈內(nèi)皮細(xì)胞         5 × 105         1ml                             

         腹膜毛細(xì)血管內(nèi)皮細(xì)胞         5 × 105         1ml                             

         食管上皮細(xì)胞         5 × 105         1ml                             

         食管平滑肌細(xì)胞     5 × 105         1ml                             

         腸微血管內(nèi)皮細(xì)胞         5 × 105         1ml                             

         腸動(dòng)脈內(nèi)皮細(xì)胞     5 × 105         1ml                             

         腸靜脈內(nèi)皮細(xì)胞     5 × 105         1ml                             

         小腸平滑肌細(xì)胞     5 × 105         1ml                             

         結(jié)腸平滑肌細(xì)胞     5 × 105         1ml