1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！【人蛋白S(ProteinS)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人蛋白S(ProteinS)ELISA試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人蛋白S(ProteinS)ELISA試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

以上資料僅供參考了解，不供于論文寫作

【人蛋白S(ProteinS)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

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    └ 腦靜脈血管內(nèi)皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 腦靜脈血管平滑肌細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 腦膜細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 神經(jīng)元細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 神經(jīng)膠質(zhì)細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 海馬神經(jīng)元細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 腦微血管內(nèi)皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 腦成纖維細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 神經(jīng)小膠質(zhì)細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 雪旺氏細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 骨細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 滑膜細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 骨骼肌細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                    【人蛋白S(ProteinS)ELISA試劑盒現(xiàn)貨】

    └ 表皮角化細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 真皮成纖維細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 軟骨細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 破骨細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 皮膚肥大細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 前脂肪細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 胰島β細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 胰腺星狀細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 胰腺導(dǎo)管上皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 頜下腺上皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 視網(wǎng)膜微血管內(nèi)皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 小梁網(wǎng)細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 視網(wǎng)膜色素上皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                   

    └ 視網(wǎng)膜muller細(xì)胞總DNA   1μg/1μl（10μg/20ug/50ug）                   

    └ 虹膜色素上皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 晶狀體上皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                    【人蛋白S(ProteinS)ELISA試劑盒現(xiàn)貨】

    └ 角膜上皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                   

    └ 角膜內(nèi)皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）