檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

9021101  Skin Panel, Lower Left Rear, DCU             CNY                             

9238279  PAXgene 96 Incubator Block (farbl.elox)          CNY                              【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】

9240677  Bumper, Round, Latch Lock, AP98            CNY                             

9240635  Filter, EMI,Modular, RJ45, AP196/98                 CNY                             

9240615  Fuse, 1.0A, FNM, AP96             CNY                             

9240612  Turk Sensor, Waste Bottle Level, AP                  CNY                             

9240603  Deck/Waste Overflow Sensor, AP196/98           CNY                             

9240583  Regulator, Pressure, Main, AP                   CNY                             

9240545  Hatch pad, Eppendorf, AP196/98               CNY                             

9240542  Lower Header, Capper Head, AP196                 CNY                             

9240521  Motor Assy, Shaker, AP98                  CNY                             

9240520  Motor Assy, Sz. 17, Common, AP98                   CNY                             

9240102  Re-Installation, LiquiChip          The Re-Installation, LiquiChip facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9019940  Power supply, vac pump, CX1           CNY                             

9019477  LASER MODUAL GS          CNY                             

9019476  Spacer, 0.2 mm short set, GSC                   CNY                             

9238888  PCB, Main-01, BS96                   CNY                             

9238886  Cable, HM, BS96               CNY                              【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】

9238854  #BR M96, Basic Agreement    The Basic Cover Agreement M96 provides full service coverage for the BioRobot M96. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238842  RS232 box, non-online fill, RapidPlate               CNY                             

9241215  Full Agreement Depot Repair, Shaker, 1yr       "The Full Agreement Depot Repair, Shaker provides full service coverage for the plate shaker used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9238833  #BR 9604, Basic Agreement    The Basic Cover Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238832  BR 9600, Basic Agreement      The Basic Cover Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238819  Rect. carrier for 5 glass tubes 25ml                   CNY                             

9237527  Software LiquiChip Workstation IS V2.2.          CNY                             

9237380  Installation, BR MDx & 2 react. setup     Installation ａnd training, including two customized reaction setup protocols         CNY                             

9237379  Installation, BR MDx & 1 react. setup     Installation ａnd training, including one customized reaction setup protocol CNY                             

9237378  BR MDx, Installation        The Installation, BR MDx facilitates the quick implementation of the BioRobot MDx into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot MDx instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9237175  Guard Fan, Platic XYP                CNY                             

9233209  Tubing, 3/2,                CNY                             

9233155  Power Cord, European              CNY                             

9236418  Board, CPU, TWI               CNY                             

9236345  Red LSR             CNY                             

rhIGF-I      重組人IGF-I    "Recombinant Human IGF-I

"       20µg                                    

rHuDES1-3/IGF1       重組人 DES1-3/ 胰島樣生長因子 -1   "Recombinant Human DES1-3/ Insulin-Like Growth factor 1

"       20μg                                    

rhIGF-BP3         重組人 IGF-BP3      "Recombinant Human IGF-BP3

"       5µg                             

rhLR3IGF-1       重組人長R3胰島樣生長因子-1   "Recombinant Human Long R3 Insulin-like Growth factor-1

"       20µg                                    

rhEGF       重組人表皮生長因子     "Recombinant Human Epidermal Growth Factor

"       100µg                                  

rhVEGF165       重組人血管內(nèi)皮生長因子165      "Recombinant Human VEGF165

"       2µg                             

rhNT-4      重組人神經(jīng)營養(yǎng)因子-4 "Recombinant Human Neurotrophin-4

"       2µg                             

rhCNTF     重組人睫狀神經(jīng)營養(yǎng)因子     "Recombinant Human Ciliary Neurotrophic Factor

"       5µg                             

rhNRG-1  重組人 NRG-1（表皮生長因子樣結(jié)構(gòu)域）         "Recombinant Human NRG-1(EGF-like domain)

"       10µg                                     【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】

rhErbB3-f 重組人ErbB3片段 "Recombinant Human ErbB3 Fragment

"       5µg                             

rhBTC       重組人乙胞素         "Recombinant Human Betacellulin

"       5µg                             

rhBMP-2  重組人骨形成蛋白-2      "Recombinant Human Bone Morphogenetic Protein-2

"       2µg                             

rhBMP-4  重組人骨形成蛋白-4      "Recombinant Human Bone Morphogenetic Protein-4

"       2µg                             

rhBMP-7  重組人骨形成蛋白-7      "Recombinant Human Bone Morphogenetic Protein-7

"       2µg                             

rhOPG-Fc 重組人骨保護(hù)素Fc融合蛋白        "Recombinant Human Osteoprotegerin/Fc Chimera

"       10µg                                    

rhNOGGIN        重組人NOGGIN蛋白     "Recombinant Human NOGGIN

"       5µg                             

rhGH         重組人生長激素     "Recombinant Human Growth Hormone

"       20µg                                    

rmGM-CSF        重組鼠粒細(xì)胞-巨噬細(xì)胞集落刺激因子        "Recombinant Murine Granulocyte- Macrophage Colony Stimulating Factor

"       5µg                             

rmTNF-α         重組鼠腫瘤壞死因子-α        "Recombinant Murine Tumor Necrosis Factor-alpha

"       5µg                             

rmLIF        重組鼠白血病抑制因子         "Recombinant Mouse Leukemia inhibitory factor

"       5µg                             

rmbFGF    重組鼠成纖維細(xì)胞生長因子-堿性        "Recombinant Murine Fibroblast Growth Factor-basic

"       10µg                                    

rmFGF-7/KGF    重組鼠成纖維細(xì)胞生長因子-7      "Recombinant Mouse Fibroblast Growth Factor-7

"       2µg                             

rmEGF      重組鼠表皮細(xì)胞生長因子     "Recombinant Murine Epidermal Growth Factor

"       100µg                                  

rmVEGF120      重組鼠血管內(nèi)皮生長因子120      "Recombinant Murine Vascular Endothelial Growth Factor 120

"       2µg                             

rmVEGF165      重組鼠血管內(nèi)皮生長因子165      "Recombinant Murine Vascular Endothelial Growth Factor 165

"       2µg                              【人α1β糖蛋白(α1β-GP)ELISA試劑盒現(xiàn)貨】

rmNOGGIN      重組鼠NOGGIN      "Recombinant Murine NOGGIN

"       5µg                             

rmCXCL16         重組鼠CXCL16         "Recombinant Murine CXCL16

"       5μg                             

rrGM-CSF 重組鼠粒細(xì)胞-巨噬細(xì)胞集落刺激因子        "Recombinant Rat Granulocyte- Macrophage Colony Stimulating Factor

"       5µg                             

rrTNF-α 重組鼠腫瘤壞死因子-α        "Recombinant Rat Tumor Necrosis Factor-alpha

"       5µg                             

rr bFGF     重組鼠成纖維細(xì)胞生長因子-堿性        "Recombinant Rat Fibroblast Growth Factor-basic

"       10µg                                    

rrIGF         重組鼠IGF-I    "Recombinant Rat IGF-I

"       10µg