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齊一生物現(xiàn)貨供應(yīng)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購(gòu)買(mǎi)任意ELISA試劑盒都享受免費(fèi)代測(cè)服務(wù)，更多進(jìn)口原裝品牌試劑盒供應(yīng)齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

【人血管生成素2(ANG-2)ELISA試劑盒廠家】酶法檢測(cè)試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【人血管生成素2(ANG-2)ELISA試劑盒廠家】操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說(shuō)明書(shū)儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開(kāi)蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【人血管生成素2(ANG-2)ELISA試劑盒廠家】齊一生物客戶(hù)寄標(biāo)本時(shí)需注明以下情況：

1、標(biāo)本編號(hào)；2、所測(cè)項(xiàng)目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實(shí)驗(yàn)后標(biāo)本是否寄回。

客戶(hù)必知：

客戶(hù)應(yīng)對(duì)所提供的材料及信息負(fù)責(zé)，如因客戶(hù)提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶(hù)承擔(dān)。

齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話(huà)也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人血管生成素2(ANG-2)ELISA試劑盒廠家】齊一生物供應(yīng)，以上資料供參考如有疑問(wèn)需要詳細(xì)資料請(qǐng)聯(lián)系我們客服人員，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9239125  Cable ethernet crossover,  Kloehn                   CNY                             

9239089  User Manual, BioSprint 15                CNY                             

9232687  Hood f. Tip Disposal Station 2.01              CNY                             

9232627  Bottle, 500-ml, white luer adapter          CNY                             

9232558  Vacuum manifold, blanks, 6S            CNY                             

9238976  Tip ａnd Sample Rack, V2          CNY                             

9238787  Angle rotor 30 x 1.5 / 2.2 ml             CNY                             

9020318  PM kit, C18 & QIAxtractor, CX1                 CNY                             

9020317  PM kit, C18 & QIAxtractor (HEPA), CX1            CNY                              【人血管生成素2(ANG-2)ELISA試劑盒廠家】

9017245  Microplate Station 8000, seesaw             CNY                             

9020226  Belt, X-axis assembly, C4x                  CNY                             

9020211  Switch, assembly, lid, Nov08-Jun09, C1x           CNY                             

9020210  Foot assembly, QGX          CNY                             

9020154  USB Connector&Cable; RGQ             CNY                             

9020091  Hall Sensor Board, TLyser 2               CNY                             

9239514  EX SD Controller                CNY                            

9239512  EX XY, Platter Interconnect               CNY                             

9239509  EX XY, Controller Board XYP              CNY                             

9236529  Tubing Guide, Probe Holder, BR8 RD                CNY                             

9236512  #BR 9604, PM Service      The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9232778  Card, x-main, PCON, 2               CNY                             

9239314  Reservoir, OLF, with sensor, RapidPlate            CNY                             

9236449  Travel Charge, zone D               CNY                             

9236434  Sensor, rotary home, TWI                  CNY                             

9239174  Cooling Block , 24-tube, 1.5 ml, tall                   CNY                              【人血管生成素2(ANG-2)ELISA試劑盒廠家】

9232634  Bottle, 250-ml, blue fitting                CNY                             

9017157  BR MDx CTM 48 PCR Setup, PRO              CNY                             

9232475  Cover, SIS, BR9604, V1.01                 CNY                             

9232334  Cable, magnet valve, DISU, BR8000                 CNY                             

9232331  Cable, power, dilutor 5-8, BR8000            CNY                             

9238779  Agreement, MDx, 2PM, 1yr    An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.          CNY                             

9238773  Project Pack     Includes 12 working days (on-site or off-site work) by a QIAGEN Instrumentation Service Specialist, valid for 3 years.  These days can be used for writing and/or optimization of protocols or for training. It includes all travel ａnd labor charges. Parts, consumable chemistries, ａnd accessories are not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.       CNY                             

9238911  Power supply 40W, BS96          CNY                             

QY-L0346 1,5-O-二咖啡�？鼘幩�30964-13-7        Cynarin     30964-13-7       20mg                          

QY-L0347 1,3-O-二咖啡�？鼘幩�1182-34-9 Cynarin     1182-34-9         20mg                           【人血管生成素2(ANG-2)ELISA試劑盒廠家】

QY-L0348 綠原酸327-97-9      Chlorogenic acid       327-97-9  20mg                          

QY-L0349 新綠原酸906-33-2 Neochlorogenic acid         906-33-2  20mg                          

QY-L0350 隱綠原酸905-99-7 Cryptochlorogenic acid    905-99-7  20mg                          

QY-L0351 異綠原酸A2450-53-5     Isochlorogenic acid A       2450-53-5         20mg                          

QY-L0352 異綠原酸B14534-61-3   Isochlorogenic acid B       14534-61-3       20mg                          

QY-L0353 異綠原酸C32451-88-0   Isochlorogenic acid C        32451-88-0       20mg                         

QY-L0354 冬綠苷490-67-5      Gaultherin        490-67-5  20mg                          

QY-L0355 甜葉懸鉤子苷64849-39-4    Rubusoside       64849-39-4     20mg                          

QY-L0356 乙氧基白屈菜紅堿152743-19-6   Ethoxychelerythrine         152743-19-6    20mg                          

QY-L0357 紫萁酮     Osmundacetone                20mg                          

QY-L0358 二氫姜黃素     Dihydrocurcumin               20mg                          

QY-L0359 姜黃素458-37-7      Curcumin         458-37-7  20mg                          

QY-L0360 去甲氧基姜黃素22608-11-3 Demethoxycurcumin        22608-11-3       20mg                          

QY-L0361 雙去氧基姜黃素24939-16-0 Bisdemethoxycurcumin   24939-16-0       20mg                          

QY-L0362 二苯乙烯苷82373-94-2 2,3,5,4-tetrahydroxyl diphenylethylene-2-o-glucoside    82373-94-2       20mg                          

QY-L0363 3,29-二苯甲�；闃侨嗜�873001-54-8   3,29-Dibenzoyl rarounitriol      873001-54-8    20mg                           【人血管生成素2(ANG-2)ELISA試劑盒廠家】

QY-L0364 β，β’-二甲基丙烯酰阿卡寧34539-65-6         β, β-dimethyl-acry-lalkannin       34539-65-6       20mg                          

QY-L0365 紫草素517-89-5      Shikonin   517-89-5  20mg                          

QY-L0366 紫草氰苷63492-69-3      Lithosprmoside         63492-69-3       20mg                          

QY-L0367 二氫辣椒素19408-84-5 Dihydrocapsaicin      19408-84-5       20mg                          

QY-L0368 辣椒素2444-46-4   Capsaicin         2444-46-4         20mg                          

QY-L0369 天然辣椒素404-86-4      Capsaicinoids   404-86-4  20mg                          

QY-L0370 槲皮素6151-25-3 117-39-5    Quercetin        6151-25-3 117-39-5 20mg                          

QY-L0371 二氫槲皮素480-18-2      Taxifolin   480-18-2  20mg                          

QY-L0372 槲皮苷522-12-3      Quercitrin         522-12-3  20mg                          

QY-L0373 異槲皮苷21637-25-2      Isoquercitrin    21637-25-2       20mg                          

QY-L0374 槲皮素-7-O-葡萄糖苷491-50-9      Quercetin-7-O-β-D-glucopyranoside      491-50-9  20mg                          

QY-L0375 槲皮素-3-O-β-D-葡糖糖-7-O-β-D-龍膽雙糖苷   Quercetin-3-O-β-D-glucopyranosyl-7-O-β-D-gentiobiosid             20mg                           

QY-L0376 楊梅素529-44-2      Myricetin 529-44-2  20mg                          

　　一般規(guī)定抑制率≥50%為陽(yáng)性，＜50%為陰性。

 定量測(cè)定

　　ELSIA操作步驟復(fù)雜，影響反應(yīng)因素較多，特別是固相載體的包被難達(dá)到各個(gè)體之間的一致，因此在定量測(cè)定中，每批測(cè)試均須用一系列不同濃度的參考標(biāo)準(zhǔn)品在相同的條件下制作標(biāo)準(zhǔn)曲線。測(cè)定大分子量物質(zhì)的夾心法ELISA，標(biāo)準(zhǔn)曲線的范圍一般較寬，曲線點(diǎn)的吸光度可接近2.0，繪制時(shí)常用半對(duì)數(shù)紙，以檢測(cè)物的濃度為橫坐標(biāo)，以吸光度為縱坐標(biāo)，將各濃度的值逐點(diǎn)連接，所得曲線一般呈S形，其頭、尾部曲線趨于平坦，中央較呈直線的部分是*理想的檢測(cè)區(qū)域。

　　測(cè)定小分子量物質(zhì)常用競(jìng)爭(zhēng)法，其標(biāo)準(zhǔn)曲線中吸光度與受檢物質(zhì)的濃度呈負(fù)相關(guān)。標(biāo)準(zhǔn)曲線的形狀因試劑盒所用模式的差別而略有不同。ELISA測(cè)定的標(biāo)準(zhǔn)曲線注意圖中橫坐標(biāo)為對(duì)數(shù)關(guān)系，這更有利于測(cè)定系統(tǒng)的表達(dá)。

        講述完了以上ELISA實(shí)驗(yàn)的操作要點(diǎn)，可以發(fā)現(xiàn)其實(shí)很多步驟都并不復(fù)雜，重要的是操作的時(shí)候一定要注重細(xì)節(jié)，熟能生巧，才可以掌握ELISA實(shí)驗(yàn)的精髓所在。

9020713  Rail guide, x axis, C34                CNY                             

9020712  Rail, x axis, C34                  CNY                             

9020679  Rail, left, CX1            CNY                             

9020642  Waste chute, straight, SP, Cabinet Qsym                   CNY                             

9020637  Shelf, consumables, Cabinet Qsym            CNY                             

9020601  Shipping Box - DML2000           CNY                             

9020488  PCB, set, GSC            CNY                             

9020484  Pulley, GSC                 CNY                             

9017596  Controller, temp.TTM-004(SN274or higher)             CNY                             

9017594  BGR Tube Holder 5 & 6 ml                 CNY                              【人血管生成素受體/含免疫球蛋白樣環(huán)和上皮生長(zhǎng)因子樣域酪氨酸激酶1(Tie1)ELISA試劑盒現(xiàn)貨】

9020257  Kit, screws, Qsym              CNY                             

9020245  QIAsymphony Cabinet AS                   CNY                             

9017344  CD User Manual LiquiChip 200                   CNY                             

9020240  PM kit, C18 & QIAxtractor (UV), CX1                CNY                             

9020219  Cable, HEPA filter, QGX             CNY                             

9020192  PCB, vac pump (incl. cable), C18               CNY                             

9239542  Assy, XY, Reflective Sensor                 CNY                             

9239528  Ferrule,Flangeless,BlueETFE,1/16tub(10)                  CNY                             

9020184  Gasket, centrifuge lid, QIAC              CNY                             

9020157  Bearing, rotor shaft, RG3                  CNY                             

9020153  8 Way Cable for LED Assy, RGQ                 CNY                             

9020147  Rotor-Gene ScreenClust HRM Software           CNY                             

9020134  Vacuum Bottle ａnd cap, PyroVW              CNY                             

9020095  Right Ratchet Holder, TLyser 2                  CNY                             

9020087  Packaging, TLyser              CNY                             

9020082  Left Ratchet Holder, TLyser               CNY                              【人血管生成素受體/含免疫球蛋白樣環(huán)和上皮生長(zhǎng)因子樣域酪氨酸激酶1(Tie1)ELISA試劑盒現(xiàn)貨】

9239511  XY, Platter Interconnect           CNY                             

9239347  Spotlight bulb (2), BR M96                CNY                             

9236494  LiquiChip, Full Agreement        The Full Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY                             

9232727  Block, 4-way, v1.02           CNY                             

9236437  Service, in-house, 1 day            CNY                             

9236428  Sensor, vertical home, TWI               CNY                             

9239150  Support, pod fixation (to base), TWII                CNY                             

QY-L0315 滇烏頭70578-24-4 Yunaconitine；Guayewuanine B     70578-24-4       20mg                           【人血管生成素受體/含免疫球蛋白樣環(huán)和上皮生長(zhǎng)因子樣域酪氨酸激酶1(Tie1)ELISA試劑盒現(xiàn)貨】

QY-L0316 (-)-丁香樹(shù)脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷136997-64-3         (-)-Syringaresnol-4-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside       136997-64-3    20mg                          

QY-L0317 丁香樹(shù)脂醇雙葡萄糖苷         Syringaresinol-di-O-glucoside            20mg                          

QY-L0318 斷血流皂苷A 916347-31-4    Clinodiside A     916347-31-4    20mg                          

QY-L0319          Cannabisin A              20mg                          

QY-L0320 杜鵑素24211-30-1 Farrerol    24211-30-1       20mg                          

QY-L0321 正丁基苯酞6066-49-5   3-n-Butylphathlide   6066-49-5         20mg                          

QY-L0322 丁烯基苯酞551-08-6      n-Butylidenephthalide     551-08-6  20mg                          

QY-L0323 洋川芎內(nèi)酯A62006-39-7        Senkyunolide A         62006-39-7       20mg                          

QY-L0324 藁本內(nèi)酯4431-01-0        Ligustilide         4431-01-0         20mg                          

QY-L0325 大蒜素（UV98%)539-86-6      Allicin        539-86-6  20mg                          

QY-L0326 蒜氨酸556-27-4      (S)-3-(Allylsulphinyl)-L-alanine  556-27-4  20mg                          

QY-L0327 大戟二烯醇514-47-6      Euphol      514-47-6  20mg                          

QY-L0328 燈盞花甲素    apigenin-7-O-glucronide                20mg                          

QY-L0329 野黃芩苷27740-01-8      Scutellarin        27740-01-8       20mg                          

QY-L0330 高黃芩素529-53-3 Scutellartln       529-53-3  20mg                          

QY-L0331 德?tīng)査?/span>6836-11-9      Deltaline  1803152  20mg                           【人血管生成素受體/含免疫球蛋白樣環(huán)和上皮生長(zhǎng)因子樣域酪氨酸激酶1(Tie1)ELISA試劑盒現(xiàn)貨】

QY-L0332 麥冬皂苷A 11054-24-3  Ophiopogonin A        11054-24-3    20mg                          

QY-L0333 麥冬皂苷B 38971-41-4  Ophiopogonin B        38971-41-4    20mg                          

QY-L0334 麥冬皂苷C911819-08-4         Ophiopogonin C       911819-08-4   20mg                          

QY-L0335 麥冬皂苷D945619-74-9          Ophiopogonin D       945619-74-9    20mg                          

QY-L0336 短葶山麥冬皂苷C87480-46-4       Liriope muscari baily saponins C       87480-46-4     20mg                          

QY-L0337 山麥冬皂苷B Liriopesides B            20mg                          

QY-L0338 魯斯可皂苷元472-11-7 Ruscogenin       472-11-7  20mg                          

QY-L0339 多舌飛蓬苷     6’-O-Caffeoylerigeroside                 20mg                          

QY-L0340 20830-75-5       Digoxin     20830-75-5       20mg                          

QY-L0341 莪術(shù)醇4871-97-0   Curcumol 4871-97-0         20mg                          

QY-L0342 莪術(shù)烯   Curzerene                20mg                          

QY-L0343 莪術(shù)二酮13657-68-6      Germacr-1(10)-ene-5,8-dione 13657-68-6       20mg                          

QY-L0344 1，8-二羥基蒽醌117-10-2    1,8-Dihydroxyanthraqui  117-10-2  20mg                          

QY-L0345 3-O-咖啡酰奎寧酸（97%）327-97-9           3-O-caffeoylquinic acid   327-97-9         20mg