檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

967852     RNeasy 96 Universal Tissue 8000 Kit (12)        Kit to purify RNA from any tissue including difficult tissues (fatty ａnd fiber-rich) in 96-well mini format. Performed on the BioRobot Gene Expression System or BR8000 (research version.Can also be done manually using manul version of kit. Uses both QIAzol phenolic reagent + RNeasy 96 plates.       CNY                             

133181     NeXtalStock Citric acid pH 3.2 (200)                  CNY                             

9018537  Sensor,  touch screen, Qsym           CNY                             

9018484  External Tube Rack, EZ1a                  CNY                             

133108     NeXtalStock Sodium bromide (200)          CNY                             

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9001868  Rotor-Gene Q 5plex HRM (PC) PrioPLUS "Rotor-Gene Instrument

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953336     MagAttract DNA Mini M48 Kit (192)       For 192 DNA preps: MagAttract Suspension B, Buffers, Proteinase K     CNY                              【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】

9018460  Pilot valve, QX           CNY                             

9015465  Interlock switch 1, for door               CNY                             

204952     QuantiFast Multiplex RT-PCR +R Kit (80)          CNY                             

133175     NeXtalStock Citric acid pH 2.0 (200)                  CNY                             

291023     mericon GMO Screen 35S-pat  (24)                 CNY                             

9001700  Rotor-Gene Q 5Plex no PC                 CNY                             

9012345  SamTrak positive sample identification             CNY                             

1067926  Buffer FG1 (825 ml)          CNY                             

133085     NeXtalStock PEG 4,000 (200)            CNY                             

38021       MAL Cartridge (1ml)                  CNY                             

9015362  Cable, T-nt comm.             CNY                             

133162     NeXtalStock CAPS pH 10.8 (200)               CNY                             

133160     NeXtalStock CAPS pH 10.4 (200)               CNY                             

133069     NeXtalStock MOPS (200)          CNY                             

133067     NeXtalStock Methanol (200)             CNY                             

922902     LiquiChip System Fluid      LiquiChip System Fluid (10x concentrate, 5 liters), for 50 liters working solution    CNY                             【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】

290053     mericon VTEC stx1/2 Kit (24)             CNY                             

290025     mericon L. monocytogenes Kit (96)                   CNY                             

204454     QuantiFast Probe RT-PCR Kit (400)           CNY                             

204345     QuantiTect Probe PCR Kit (1000x50µl)    20For 1000 x 50 ul reactions: 25 ml QuantiTect Probe PCR Master Mix (providing a final concentration of 4 mM MgCl2), 20 ml RNase-free water        CNY                             

9015358  Inverter, UV lamp             CNY                             

9015340  Board, SGB                CNY                             

380615     Investigator Hexaplex ESS Kit (100)                   CNY                             

9001421  QIAxcel    "long

descr"       CNY                             

130917     NeXtal DWBlock ComPAS Suite                  CNY                             

QC024      包涵體溶解液         50ml                                              

QC025      蛋白復(fù)性液     100ml                                           

QC026      蛋白標(biāo)準(zhǔn)(5mg/ml BSA)  1ml                                        【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】

QC027      PMSF(100mM)          1ml                                       

QC028      5×Tris-甘氨酸電泳緩沖液    250ml                                           

QC029      SDS-PAGE電泳液(10X)    100ml                                           

QC030      SDS-PAGE蛋白上樣緩沖液(5X)      2ml                                       

QC031      非變性PAGE蛋白上樣緩沖液(5X)         2ml                                       

QC032      10%過硫酸銨 1.5ml                                            

QC033      30% 丙烯酰胺 (29:1)    100ml                                           

QC034      40% 丙烯酰胺 (39:1)    100ml                                           

QC035      0.5M DTT         2ml                                       

QC036      10% SDS   50ml                                              

QC037      TEMED     5ml                                       

QC038      1M Tris-HCl,pH6.8   50ml                                              

QC039      1.5M Tris-HCl,pH8.8         100ml                                           

QC040      Western轉(zhuǎn)膜緩沖液 （20x）       100ml                                           

QC041      Western一/二抗稀釋液          100ml                                           

QC042      Western封閉液(BSA型）      100ml                                            【人熱休克因子1(HSF-1)ELISA試劑盒現(xiàn)貨】

QC043      Western洗滌液       250ml                                           

QC044      Western一抗二抗去除液       250ml                                           

QC045      BCIP工作液 50mg/ml    100ul                                             

QC046      NBT工作液 50mg/ml    100ul                                             

QC048      10% Sodium Azide (疊氮鈉)    100ml                                           

QC049      Protease Inhibitor  A(廣譜性)        1ml                                       

QC050      Protease Inhibitor  B(適用于細(xì)菌)        1ml                                       

QC051      Protease Inhibitor  C(適用于哺乳動(dòng)物)        1ml                                       

QC052      Protease Inhibitor  D(適用于真菌)       1ml                                       

QC053      Protease Inhibitor  E(適用于哺乳動(dòng)物)        1ml                                       

QC054      Phosphatase Inhibitor M(廣譜性)   1ml                                       

QC055      胰酶細(xì)胞消化液     100ml                                           

QC015      植物細(xì)胞質(zhì)膜提取試劑盒     50T                                       

QC016      植物蛋白提取試劑盒     50T                                       

QC017      細(xì)菌總蛋白提取試劑盒         50T                                       

QC018      酵母總蛋白提取試劑盒         50T                                       

QC019      胞漿和核蛋白分步提取試劑盒     50T                                       

QC020      膜蛋白提取試劑盒         50T                                       

QC021      動(dòng)物組織蛋白裂解液     50ml                                              

QC022      真核細(xì)胞蛋白裂解液     50ml                                              

QC023      RIPA裂解液   50ml