1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類(lèi)戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購(gòu)咨詢(xún)，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫(xiě)作

齊一生物專(zhuān)業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

79523       Small Spin Columns  24 RNase-free spin columns for Oligotex spin procedures       CNY                             

76544       RNAprotect Animal Blood Tubes 100µl (50)              CNY                             

74324       RNeasy Protect Saliva Mini Kit (50) "The RNeasy Protect Saliva Kit is a D-BOM: RNAprotect Saliva Reagent (50ml) #1037475

RNeasy Micro kit (50)  #74004."   CNY                             

19590       InhibitEX (100)          CNY                             

70042       Oligotex mRNA Midi Kit  For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents ａnd Buffers   CNY                             

69984       TissueLyser Adapter Set (2 x 96)      2 sets of Adapter Plates for use with Collection Microtubes (racked) on the TissueLyser         CNY                             

69582       DNeasy 96 Blood & Tissue Kit (12)  For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

19581       96-Well Microplates (24) 96-well microplates with round-bottom wells plus lids, 24 per case, for use with QIAvac manifolds ａnd the BioRobot 9600    CNY                             

19418       QIAvac Holder Kit             CNY                             

19408       VacValves (24) For processing QIAamp mini spin columns on QIAvac 24 or QIAvace 24 Plus: 24 VacValves. Should be orded in conjunction with VacConnectors (500) - Catalog No. 19407.  CNY                              【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】

19201       Collection Tubes (2-ml)     1000 Collection Tubes (2 ml)    CNY                             

19112       Buffer MTL (54 ml)            CNY                             

19046       Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps CNY                             

16191       QIAwell 96 Ultra Plasmid Kit (4)       For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, ａnd QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps     CNY                             

12991       QIAGEN Plasmid Plus Giga Kit (5)    QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

12863       CompactPrep Plasmid Maxi Kit (25)         "For ultrafast purification of up to 200 µg or 750 µg Molecular Biology Grade plasmid or cosmid DNA for standard applications

CompactPrep Plasmid Kits enable fast, large-scale plasmid purification in a miniprep format. The use of a vacuum manifold allows 24 samples to be purified in parallel on a single workbench"       CNY                             

133281     NeXtalStock TRICINE pH 8.5 (200)            CNY                             

133275     NeXtalStock Spermidine (200)          CNY                             

337611     qBiomarker SYBR Green Mastermix (8)           CNY                             

4501303  artus EBV QS-RGQ Kit (24) RUO                CNY                             

4500363  artus HSV-1/2 QS-RGQ Kit (24) CE              CNY                             

949006     Autopure Cell Lysis Solution (3800 ml)               CNY                             

949002     Autopure Glycogen Solution (5 ml)           CNY                             

336313     SureSilencing shRNA Plasmid, Neomycin                   CNY                             

990382     Sample Tubes CB (2ml)              CNY                             

937255     QIAsymphony DSP DNA Midi Kit               CNY                              【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】

9010815  Cable, card-X to bar-code camera BL180                  CNY                             

330619     RT² SYBR Green Fluor FAST Mastermix 25ml            CNY                             

133245     NeXtalStock Sodium malonate pH 7.0 (200)             CNY                             

929703     QX 0.2 ml 12-Tube Strip (80)             CNY                             

330512     RT² SYBR Green Fluor qPCR Mastermix (12)             CNY                             

330141     Custom RT² PreAMP Primer Mix               CNY                             

9010603  Anschlagleiste fuer Samtrak P7xxx (9194)                 CNY                             

QT101-6   TUREscript One Step RT-PCR Kit（一步法反轉(zhuǎn)錄試劑盒）        2050                                               【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】

QT101-8   TUREscript SY Green qRT-PCR Kit （兩步法熒光定量反轉(zhuǎn)錄試劑盒）      2050                                              

QD101-1  DNA MarkerⅠ（條帶100.200.300.400.500.600）      "50T

"                                            

QD101-3  DNA Marker Ⅱ（條帶100.300.500.700.900.1200） "50T

"                                            

QD101-5  DNA Marker Ⅲ（條帶300.500.800.1500.2000.3000.5000）     "50T

"                                            

QD101-7  DNA Marker Ⅳ（條帶500.1000.1500.3000.5000.8000） "50T

"                                            

QD101-9  DNA Marker Ⅴ（條帶200.400.700.1000.1500.2000）      "50T

"                                            

QD101-11         DNA MarkerⅥ(條帶250.1000.2500.5000.7000.10000)        "50T

"                                           

QD101-13         DNA Marker Ⅶ（條帶300.500.1000.1500.2000.2500）    "50T

"                                            

QD101-17         50bp DNA Marker（條帶50.100.150.200.250.300.250.400）     "50T

"                                            

QD101-19         50bp Plus（條帶50.100.150.200.250.300.350.400.500.600.1000）   "50T

"                                            

QD101-21         100bp DNA Marker（100.200.300.400.500.600.700.800.900.1000.1500）         "50T

"                                            

QD101-23         100bp Plus（100.200.300.400.500.600.700.800.900.1000.1500.2000.3000.5000）         "50T

"                                            

QD101-25         200bp DNA Ladder  "50T

"                                            

QD101-27         250bp DNA Ladder（條帶250.500.750.1000.1500.2000.2500.3000.5000）       "50T

"                                            

QD101-29         300bp DNA Ladder（條帶300.600.900.1200.1500.1800.2500.3000.5000）       "50T

"                                            

QD101-31         500bp DNA Ladder（500.1000.1500.2000.2500.3000.3500.5000）   "50T

"                                             【人血管生成素4(ANG-4)ELISA試劑盒現(xiàn)貨】

QD101-33         1kb  DNA Marker（條帶1000.2000.3000.4000.5000.6000.8000.10000）        "50T

"                                            

QD101-35         1kb Plus（條帶300.500.800.1000.1500.2000.3000.4000.5000.6000.8000.10000）  "50T

"                                            

QD101-43         DL2000（條帶100.250.500.750.1000.2000）      "50T

"                                            

QD101-45         DL2000  Plus                                    （條帶100.250.500.750.1000.2000.3000.5000） "50T

"                                            

QH406-1  λDNA/HindⅢ         "50T

"                                            

QH406-3  pUC19 DNA/MspⅠ "50T

"                                            

QC101-01          DH5α感受態(tài)細(xì)胞 40×100ul                                             

QC102-01          Top10感受態(tài)細(xì)胞  40×100ul                                             

QC103-01          JM109感受態(tài)細(xì)胞 20×100ul                                             

QC104-01          BL21(DE3)感受態(tài)細(xì)胞    20×100μl                                          

QC105-01          BL21(DE3) Competent cell       20×100ul                                      

QC121-01          高效感受態(tài)細(xì)胞制備試劑盒         100×100μl                                        

QC201-1   pSURE -T 載體連接試劑盒    20次                                             

 QC201-3          pSURE-T Simple載體連接試劑盒  20次(帶T4 ligase）