1.購買我公司任意ELISA試劑盒指標(biāo)享受免費代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人瘦素受體(LEPR)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準(zhǔn)備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【人瘦素受體(LEPR)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人瘦素受體(LEPR)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【人瘦素受體(LEPR)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實驗具體詳細(xì)步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進(jìn)行階段，我公司客服人員會及時向您匯報實驗進(jìn)程。

5000-1253        Iris Control PCB, DML2              CNY                             

5050-1071 V.1 CD RomManual-DML2000 Instrument I, 240V                 CNY                             

5050-00031      CONVERTER USB               CNY                             

5000-1509        Control Heater, HTR1                CNY                             

6000-3121        Plate Washer 1 - Replacement Tubing Kit                 CNY                             

6000-3115        Plate Washer I - Manifold Accessory Kit           CNY                             

5000-1502        Fuse 250V 1A, HTR1                  CNY                             

5000-1419        Breaker Circuit 1Pole, VTX2              CNY                             

5198-1220        hc2 HPV DNA Test(High Risk ａnd Low Risk)               CNY                             

EA10224  EASYartus VZV RG_II                 CNY                             

EA10023  EASYartus HSV-1/2 RG_I            CNY                             

PK-01        Mutation Test Kit (25)               CNY                             

9240744  Belt, Reagent Pump , Long, AP96             CNY                             

9240737  Disinfectant Spray, Conflikt, AP                 CNY                              【人瘦素受體(LEPR)ELISA試劑盒廠家】

5000-1413        Knob RB673STKWL Black, VTX2                CNY                             

9240698  Cap, Waste Bottle, Modified, AP              CNY                             

9243655  Addl Unit or Protocol, Comp. Valid Supp Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Comprehensive validation service catalog no(s). Scope of work defined from the primary unit utilized in the Comprehensive Validation Support.  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.    CNY                             

1024481  #LCX Vacuum System       Holds 24 QIAamp Maxi columns includes connectors, tubing, waste bottles, vacuum gauge, filters, tray, user manual.   CNY                             

9241221  RCS, Installation       RCS Installation facilitates the quick start up of the RCS into the lab routine. It covers hardware ａnd software installation of the system ａnd provides the user with an introductory training on operation ａnd user maintenance. Installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.   CNY                             

330025     Microbial DNA qPCR Assays              CNY                             

9022438  USB Ethernet Adaptor, PyroQ96               CNY                             

274202     flocktype IBDV Ab (2)                CNY                             

270805     cattletype MAP Ab (20)            CNY                             

287015     cador M. hyopneumoniae PCR Reagent (96)            CNY                             

285815     cador Leptospira PCR Reagent (96)                   CNY                             

285205     cador B. hyodysenteriae PCR Kit (96)                CNY                             

273001     pigtype Salmonella Ab (1)                  CNY                             

9020579  Jumper, Main Board J5, DML 3000           CNY                             

9020590  PCB Assy, Controller, DML 3000                CNY                             

9015908  Upg. Kit, Tubing Protection, BR8 (ISI25)          CNY                              【人瘦素受體(LEPR)ELISA試劑盒廠家】

9235642  High-speed shaker unit, BR8000               CNY                             

9013227  Ferri-Sheld f. Y-flatband             CNY                             

9013215  ZLV Screws, plastic, M3  (20)                   CNY                             

QTR-1201         布魯氏菌抗原快速檢測試劑                                                       

QTR-1202         A型肉毒毒素快速檢測試劑                                               

QTR-1203         B型肉毒毒素快速檢測試劑                                               

QTR-1204         I型嗜肺軍團菌抗原快速檢測試劑                                                     

QTR-1205         OI群霍亂弧菌抗原快速檢測試劑                                                      

QTR-1206         沙門菌抗原快速檢測試劑                                                  

QTR-1207         瘧疾快速檢測試劑                                                       

QTR-1208         炭疽抗體快速檢測試劑                                                       

QTR-1209         鼠疫抗體快速檢測試劑                                                       

QTR-1210         土拉熱抗體快速檢測試劑                                                  

QTR-1211         類鼻疽抗體快速檢測試劑                                                  

QTR-1212         鼻疽抗體快速檢測試劑                                                       

QTR-1213         布魯氏菌抗體快速檢測試劑                                                        【人瘦素受體(LEPR)ELISA試劑盒廠家】

QTR-1214         鸚鵡熱抗體快速檢測試劑                                                  

QTR-1215         立克次體病抗體快速檢測試劑                                                  

QTR-1216         軍團菌病抗體快速檢測試劑                                                       

QTR-1217         傷寒（付傷寒）抗體快速檢測試劑                                                  

QTR-1218         腸出血性大腸桿菌抗體快速檢測試劑                                                       

QTR-1219         流行性腦脊髓膜炎抗體快速檢測試劑                                                       

QTR-1220         鉤端螺旋體抗體快速檢測試劑                                                  

QTR-1221         萊姆病伯氏疏螺旋體抗體快速檢測試劑                                                  

QY102-1   高純度質(zhì)粒小量提取試劑盒         "100T

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QY103-1   質(zhì)粒中量提取試劑盒     "100T

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QY104-1   高純度質(zhì)粒中量提取試劑盒         "100T

"                                            

QY105-1   質(zhì)粒大量提取試劑盒     "100T

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QY106-1   高純度質(zhì)粒大量提取試劑盒         "100T

"                                           

QY107-1   酵母質(zhì)粒小量提取試劑盒     "100T

"                                            

QY108-1   高純度酵母質(zhì)粒小量提取試劑盒         "100T

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QY109-1   酵母質(zhì)粒中量提取試劑盒     "100T

"                                             【人瘦素受體(LEPR)ELISA試劑盒廠家】

QY110-1   高純度酵母質(zhì)粒中量提取試劑盒         "100T

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QY111-1   酵母質(zhì)粒大量提取試劑盒     10T