齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶在不同的時(shí)間來(lái)分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營(yíng)商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長(zhǎng)期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類型。未經(jīng)檢驗(yàn)的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問(wèn)題的來(lái)源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來(lái)沒(méi)有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來(lái)很好，但我沒(méi)有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請(qǐng)檢查是否正確進(jìn)行稀釋。過(guò)稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤(pán)子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無(wú)塵紙。【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

14。我需要用一個(gè)盤(pán)子嗎？

可靠的結(jié)果可以得到無(wú)板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長(zhǎng)校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長(zhǎng)讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過(guò)程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來(lái)源。這個(gè)信息的*佳來(lái)源是目前的文獻(xiàn)，很容易通過(guò)互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫(kù)訪問(wèn)。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來(lái)了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9235161  Zylinderstift rostfrei 2 h8 x25            CNY                             

9230253  Rack for reagents, Universal (zig-zag)              CNY                             

9022779  PyroMark Q24 Advanced Software          CNY                             

9235105  Manual, user, hardware BR8000 Int                 CNY                             

9234863  Washer liquid container (9l Nalgene)                CNY                             

9238670  Wedge belt 5 x 513           CNY                             

9238648  Motorized lock with optic, right      "For Sigma centrifuge

Motor that controls the locks

Includes optical sensor"   CNY                             

9238640  Tachogenerator                 CNY                             

9242058  Tube insert, 13 mm, R, sample carr., Qsy                  CNY                             

9241809  Pyro Q96 MDa, Installation     Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements         CNY                             

9241162  Board, transport conversion, QX               CNY                             

9241146  RS232 Board, Eppendorf, AP196/98          CNY                             

9240993  Calibration Plate, MDD             CNY                             

9240962  PCB, connector, pipetting system, Qsym                   CNY                             

9240959  Activation bar tip, handler, Qsym             CNY                              【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

9240948  Cable, x, portal, Qsym               CNY                             

9240825  Field Upgrade, BR EZ1 DSP               CNY                             

9240817  #Premium Cover Agreement EZ1 DSP, 1YR     "Repair service delivery: onsite

Service response time (repair): 24 working hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9240776  Dispense Head Update, AP96           CNY                             

9022763  EZ1 Adv. XL DNA Invest. Flip-Cap Card              CNY                             

6000-5012        Lumicheck Battery Replacement Package                CNY                             

6000-5005        Micro Well Strips               CNY                             

9241774  QIAgility, Installation        The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the QIAgility systems hardware ａnd trains the user on the operation of the software. After the training the user will be able to run ａnd install application programs ａnd conduct a basic troubleshooting. All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements. CNY                             

9241655  Input Rack Pins Kit, AP              CNY                             

9241360  Plastic supporters, door hinge, EZ1a                 CNY                             

9243547  Pyro Q24, PM Service       "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243112  Hard Drive, Programmed, Cheetah, AP98                CNY                             

9242770  Belt, x-drive, portal, AS, Qsym                   CNY                             

9242698  RGQ, Full Agreement       "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included" CNY                             

5000-1406        Control PCB 120V, VTX2            CNY                             

5000-1320        Label Front Overlay, SKR1                  CNY                              【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

5000-1312        Platform, SKR1                   CNY                             

5000-1274        Plate Mask, DML2            CNY                             

QTR-1170         豬繁殖與呼吸綜合征病毒美洲型熒光RT-PCR檢測(cè)試劑盒        48                                         

QTR-1171         戊型肝炎病毒熒光RT-PCR檢測(cè)試劑盒        48                                         

QTR-1172         鯉春病熒光RT-PCR檢測(cè)試劑盒   48                                         

QTR-1173         狂犬病病毒熒光RT-PCR檢測(cè)試劑盒   48                                         

QTR-1174         傳染性造血器官壞死病病毒RT-PCR檢測(cè)試劑盒        48                                         

QTR-1175         H3N8馬流感病毒熒光RT-PCR檢測(cè)試劑盒 48                                         

QTR-1176         禽流感病毒和新城疫病毒熒光RT-PCR檢測(cè)試劑盒   48                                         

QTR-1177         禽滑液囊支原體抗體診斷試劑盒                                                       

QTR-1178         禽滑液囊支原體抗體診斷試劑盒                                                       

QTR-1179         雞新城疫病毒抗體檢測(cè)試劑盒                                                  

QTR-1180         鼠肝炎病毒抗體檢測(cè)試劑盒                                                       

QTR-1181         禽肺病毒（禽氣管炎）抗體檢測(cè)試劑盒                                                   【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

QTR-1182         羊邊界病毒抗體診斷試劑盒                                                       

QTR-1183         沙門(mén)氏菌共價(jià)混合物酶聯(lián)法抗體診斷試劑盒                                                  

QTR-1184         豬偽狂犬病毒gB抗體診斷試劑盒                                                     

QTR-1185         豬偽狂犬病毒gE抗體診斷試劑盒（gE抗體）                                               

QTR-1186         口蹄疫病毒抗體診斷試劑盒(3ABC抗體)                                                   

QTR-1187         牛布魯氏桿菌競(jìng)爭(zhēng)酶聯(lián)法抗體診斷試劑盒                                                       

QTR-1188         牛布魯氏桿菌間接酶聯(lián)法抗體診斷試劑盒                                                       

QTR-1189         牛副流感3型抗體診斷試劑盒                                                   

QTR-1190         牛呼吸道合胞體病毒抗體檢測(cè)試劑盒                                                       

QTR-1191         牛冠狀病毒抗體診斷試劑盒                                                       

QTR-1192         嗜肺軍團(tuán)菌血清組1檢測(cè)試劑盒                                                       

QTR-1193         嗜肺軍團(tuán)菌血清組2-14檢測(cè)試劑盒                                                 

QTR-1194         軍團(tuán)菌乳膠凝集試劑盒                                                       

QTR-1195         嗜肺軍團(tuán)菌主要血清組1檢測(cè)試劑盒                                                       

QTR-1196         炭疽桿菌抗原快速檢測(cè)試劑盒                                                  

QTR-1197         鼠疫菌抗原快速檢測(cè)試劑                                                   【人心肌錨蛋白重復(fù)域1(ANKRD1)ELISA試劑盒現(xiàn)貨】

QTR-1198         土拉熱菌抗原快速檢測(cè)試劑                                                       

QTR-1199         類鼻疽菌抗原快速檢測(cè)試劑                                                       

QTR-1200         鼻疽菌抗原快速檢測(cè)試劑