檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【人抗腎上腺皮質(zhì)抗體(AAA)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人抗腎上腺皮質(zhì)抗體(AAA)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人抗腎上腺皮質(zhì)抗體(AAA)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【人抗腎上腺皮質(zhì)抗體(AAA)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

204752     QuantiFast Multiplex PCR +R Kit (80)                CNY                             

290095     mericon Quant. L. pneumophila Kit (96)          CNY                             

800423     Library, Large Insert, Picked Clones                   CNY                             

290045     mericon Campylobacter triple Kit (96)              CNY                             

9015353  Controller, temp.               CNY                             

133051     NeXtalStock HEPES sod. salt untit. (200)          CNY                             

9018388  QIAcube Tip Adapter Ring Tool                 CNY                             

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9012226  Power Supply, 6 amp for Plato size 90               CNY                             

9012066  Tray, buffer bottles, roter, BR8000           CNY                             

9001674  PyroMark Q96 MD Automated Instrument     For performing automated pyrosequncing reaction on the Q96 MD      CNY                             

9001658  Rotor-Gene Q 5plex HRM (w/o PC) Priority     Rotor-Gene Q 5plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

951054     EZ1 DNA Blood 350 µl Kit (48)          "

48 Reagent Cartridges (Blood 350 ul), 50 Disposable Tip Holders, 50 Disposable Filter-Tips, 50 Sample Tubes (2.0 ml), 50 Elution Tubes (1.5 ml)"  CNY                             

9001874  EZ1 Advanced XL, System         EZ1 Advanced XL, System combines the EZ1 Advanced XL instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                              【人抗腎上腺皮質(zhì)抗體(AAA)ELISA試劑盒現(xiàn)貨】

9015466  Cover, P             CNY                             

9012413  Z-Bars Gripper, RCS1                 CNY                             

9012410  Tischplatte Plato 3301S Technikk  (9194)                CNY                             

205110     Omniscript RT Kit (10)      For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

204845     QuantiTect Multiplex RT-PCR NR Kit(1000)               CNY                             

133178     NeXtalStock Citric acid pH 2.6 (200)                  CNY                             

133165     NeXtalStock CAPS pH 11.4 (200)               CNY                             

9018409  Regulator, N2, internal, front, QX            CNY                             

9015406  Driver, motor, M96           CNY                             

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1067925  Buffer AW1 (175 ml)                  CNY                             

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38003       ConA Cartridges (3 x 1ml)                  CNY                             

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204741     QuantiTect Multiplex PCR NoROX Kit (40)       "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect Multiplex PCR NoROX Master Mix, 2.0 ml RNase-free water

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133066     NeXtalStock Manganese(II) chloride (200)                CNY                             

290065     mericon Cronobacter spp Kit (96)             CNY                             

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RND99002        鏈霉素檢測試劑盒         Streptomycin ELISA Test Kit     96T  美國REAGEN                   

RND99003        新霉素檢測試劑盒         Neomycin ELISA Test Kit  96T  美國REAGEN                   

RND99004        慶大霉素檢測試劑盒     Gentamicin (GM) ELISA Test Kit       96T  美國REAGEN                   

RND99005        硝基咪唑檢測試劑盒     Nitroimidazole ELISA Test Kit  96T  美國REAGEN                   

RND99006        泰樂菌素檢測試劑盒     Tylosin ELISA Test Kit        96T  美國REAGEN                   

RND99007        丙烯酰胺檢測試劑盒     Acrylamide-ES  ELISA Test Kit         96T  美國REAGEN                   

RND99008        孔雀石綠檢測試劑盒     Malachite Green (MG) ELISA Test Kit      96T  美國REAGEN                   

RND99009        氟喹諾酮檢測試劑盒     Fluoroquinolone (FQS) ELISA Test Kit        96T  美國REAGEN                   

RND99010        三聚氰胺檢測試劑盒     Melamine ELISA Test Kit 96T  美國REAGEN                    【人抗腎上腺皮質(zhì)抗體(AAA)ELISA試劑盒現(xiàn)貨】

RND99011        呋喃唑酮檢測試劑盒     Furazolidone (AOZ) ELISA Test Kit   96T  美國REAGEN                   

RND99012        呋喃它酮檢測試劑盒     Furaltadone (AMOZ) ELISA Test Kit 96T  美國REAGEN                   

RND99015        磺胺嘧啶檢測試劑盒     Sulfadiazine ELISA Test Kit       96T  美國REAGEN                   

RND99016        磺胺二甲基嘧啶檢測試劑盒         Sulfamethazine ELISA Test Kit 96T  美國REAGEN                   

RND99017        磺胺甲基異噁唑檢測試劑盒         Sulfamethoxazole ELISA Test Kit      96T  美國REAGEN                   

RND99018        雪卡毒素檢測試劑盒     Cigua-Check Fish Poison Test Kit      96T  美國REAGEN                   

RND99019        蘇丹紅檢測試劑盒         Sudan red Test Kit    96T  美國REAGEN                   

RND99020        環(huán)丙沙星檢測試劑盒     Ciprofloxacin ELISA Test Kit      96T  美國REAGEN                   

RND99021        氧氟沙星檢測試劑盒     Ofloxacin ELISA Test Kit   96T  美國REAGEN                   

RND99022        氯丙嗪檢測試劑盒         Chlorpromazine ELISA Test Kit         96T  美國REAGEN                   

RND99023        呋喃西林檢測試劑盒     Nitrofurazone   (SEM) ELISA Test Kit    96T  美國REAGEN                   

RND99024        呋喃妥因檢測試劑盒     Nitrofurantoin (AHD) ELISA Test Kit         96T  美國REAGEN                   

RND99025        四環(huán)素檢測試劑盒         Tetracycline (TET) ELISA Test Kit      96T  美國REAGEN                   

RND99026        牛奶總抗生素檢測試劑盒     Total Antibiotics in Milk Test Kit       96T  美國REAGEN                   

RND99027        β內(nèi)酰胺類檢測試劑盒         Beta-lactam antibiotics ELISA Test Kit     96T  美國REAGEN                   

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RND99029        氟苯尼考檢測試劑盒     Florfenicol ELISA Test Kit 96T  美國REAGEN                   

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