1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

293023     mericon Corn (24)             CNY                             

292055     mericon Goat Kit (96)                CNY                             

929521     QX Alignment Marker 15 bp/1 kb (1.5 ml)                 CNY                             

9012598  Disposable Filter-Tips, 1100µl (960)         Conducting disposable filter-tips      CNY                             

206152     QIAGEN Multiplex PCR Plus Kit (100)                CNY                             

291115     mericon RR Soy Kit (96)            CNY                              【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】

291065     mericon GMO Screen bar (96)                   CNY                             

9001933  DML 3000 120-240V Q              CNY                             

9001915  PyroMark Q96 ID, Priority        PyroMark Q96 ID, Priority includes the PyroMark Q96 ID instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133120     NeXtalStock Tricine (200)                   CNY                             

133180     NeXtalStock Citric acid pH 3.0 (200)                  CNY                             

9018503  Manual air supply, service tool, QX           CNY                             

51161       QIAamp 96 DNA Blood Kit (4) For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels   CNY                             

133106     NeXtalStock Sodium ac. H2O pH 4.6 (200)                CNY                             

9015519  Spring, for door                  CNY                             

9001897  QIAxtractor, UV/HEPA (w/o PC) Priority  QIAxtractor, UV/HEPA (w/o PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.  CNY                             

9001875  EZ1 Advanced XL, Priority        EZ1 Advanced XL, Priority includes the EZ1 Advanced XL instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

959236     MagAttract RNA Tissue Mini M48 Kit (192)             CNY                             

956336     MagAttract RNA Univ. Tissue M48 Kit(192)             CNY                             

9018457  Cartridge, alignment tool, QX          CNY                             

9015467  Motor, P            CNY                             

9015451  Modules, DISU, BR8000 v2.1            CNY                              【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】

9012427  Thermostate system, complete, 120V, BR30           CNY                             

9018483  Notebook, QX           CNY                             

51297       FlexiGene DNA AGF3000 Kit (640)  "Buffer FG1  = 4 x 825 ml

Buffer FG2 = 1 x 750 ml

Buffer FG3 = 10 x 625 ml

QIAGEN Protease = 5 vials

1 handbook"    CNY                             

51185       QIAamp DNA Blood Midi Kit (100)  For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)   CNY                             

38075       AIL Cartridge (5ml)           CNY                             

9015471  Sensor, photo, PM-U24             CNY                             

956034     EZ1 RNA Universal Tissue Kit (48)   Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous ａnd fatty tissues, or cultures cells.         CNY                             

204772     Rotor-Gene Multiplex PCR Kit (80)  The Rotor-Gene Multiplex PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly reliable quantification in multiplex, real-time PCR ａnd two-step RT-PCR using sequence-specific probes. Up to 4 genes (e.g., 1 control gene ａnd 3 target genes) can be quantified simultaneously in the same reaction.       CNY                             

9018435  Tubing, external N2 source, 10ft, QX                CNY                             

9018417  Cover, front, blue window, QX          CNY                             

9018403  ZLV, Detect barrel (1), QX                  CNY                             

QKT0671  5′-核苷酸酶測(cè)定試劑盒5′-NT連續(xù)監(jiān)測(cè)法                                                  

QKT0672  血清總膽紅素測(cè)定試劑盒T-Bil     膽紅素氧化酶法                                         

QKT0673  L-γ-谷氨�；茡Q酶測(cè)定試劑盒GGT連續(xù)監(jiān)測(cè)法                                                 

QKT0674  堿性磷酸酶測(cè)定試劑盒ALP速率法                                                   

QKT0675  前白蛋白測(cè)定試劑盒PA免疫比濁法                                                 【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】

QKT0676  尿酸測(cè)定試劑盒UA尿酸氧化酶法                                                    

QKT0677  腺苷脫氨酶測(cè)定試劑盒ADA連續(xù)監(jiān)測(cè)法                                                 

QKT0678  膽堿酯酶測(cè)定試劑盒CHE速率法                                                       

QKT0679  總膽汁酸測(cè)定試劑盒TBA循環(huán)酶法                                                  

QKT0680  總膽紅素測(cè)定試劑盒T-Bil     化學(xué)氧化法                                         

QKT0681  直接膽紅素測(cè)定試劑盒D-Bil化學(xué)氧化法                                                 

QKT0682  丙氨酸基轉(zhuǎn)移酶測(cè)定試劑盒ALT  速率法                                         

QKT0683  天門冬氨酸基轉(zhuǎn)移酶測(cè)定試劑盒AST 速率法                                         

QKT0684  肌酸激酶人N端中段骨鈣素N-MIDOsteocalcin定量檢測(cè)卡               北京華科泰                                

QKT0685  25-羥基維生素D25-OH-D定量檢測(cè)卡                                              

QKT0686  25-羥基維生素D325-OH-D3¬定量檢測(cè)卡                                                  

QKT0687  甲狀旁腺激素PTH定量檢測(cè)卡                                                  

QKT0688  全程C-反應(yīng)蛋白定量檢測(cè)卡                                                       

QKT0689  降鈣素原PCT定量檢測(cè)卡                                                   

QKT0690  肌紅蛋白MYO定量檢測(cè)卡                                                

QKT0691  肌酸激酶同工酶CK-MB定量檢測(cè)卡                                                   【人血紅素氧合酶2(HO-2)ELISA試劑盒現(xiàn)貨】

QKT0692  心肌肌鈣蛋白IcTnI定量檢測(cè)卡                                                 

QKT0693  D-二聚體D-Dimer定量檢測(cè)卡                                                   

QKT0694  N-末端腦鈉肽前體NT-proBNP定量檢測(cè)卡CK測(cè)試盒                  96T                             

QKT0697  3,5,3＇－－三碘甲腺原氨酸T3化學(xué)發(fā)光檢測(cè)試劑盒                 96T                             

QKT0698  甲狀腺素T4化學(xué)發(fā)光檢測(cè)試劑盒                 96T                             

QKT0699  游離3,5,3＇－－三碘甲腺原氨酸FT3化學(xué)發(fā)光檢測(cè)試劑盒              96T                             

QKT0700  游離甲狀腺素FT4化學(xué)發(fā)光檢測(cè)試劑盒               96T                             

QKT0701  促甲狀腺素TSH化學(xué)發(fā)光檢測(cè)試劑盒                   96T                             

QKT0702  甲狀腺過氧化物酶TPO化學(xué)發(fā)光檢測(cè)試劑盒              96T                             

QKT0703  抗甲狀腺球蛋白抗體TG化學(xué)發(fā)光檢測(cè)試劑盒           96T