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齊一生物現(xiàn)貨供應(yīng)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購買任意ELISA試劑盒都享受免費代測服務(wù)，更多進口原裝品牌試劑盒供應(yīng)齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人蛋白激酶B(PKB)ELISA試劑盒廠家】酶法檢測試驗所需自備物品:

1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【人蛋白激酶B(PKB)ELISA試劑盒廠家】操作注意事項：

1.試劑盒應(yīng)按標簽說明書儲存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過后的標準品應(yīng)丟棄，不可保存。

2.實驗中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標板時應(yīng)充分拍干，不要將吸水紙直接放入酶標反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長時間打開蓋子。底物B對光敏感，避免長時間暴露于光下。避免用手接觸，有毒。實驗完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時間一樣。

9.按照說明書中標明的時間、加液的量及順序進行溫育操作。

【人蛋白激酶B(PKB)ELISA試劑盒廠家】齊一生物客戶寄標本時需注明以下情況：

1、標本編號；2、所測項目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實驗后標本是否寄回。

客戶必知：

客戶應(yīng)對所提供的材料及信息負責，如因客戶提供的材料及信息不準確而引起的實驗延誤或經(jīng)濟損失由客戶承擔。

齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人蛋白激酶B(PKB)ELISA試劑盒廠家】齊一生物供應(yīng)，以上資料供參考如有疑問需要詳細資料請聯(lián)系我們客服人員，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

301107     PolyFect Transfection Reagent(4 x1.0 ml)        "

For 100-260 transfections in 60 mm dishes or 200-400 transfections in 6-well plates." CNY                             

54104       QIAamp cador Pathogen Mini Kit (50)              CNY                             

9001946  QIAcube (220-240V) Promo              CNY                             

971061     therascreen® MGMT Pyro Kit (48)           CNY                             

293035     mericon Apricot kernels (96)             CNY                             

929520     QX Alignment Marker 15bp/500bp (1.5 ml)              CNY                             

9012643  Bottle, system liquid                  CNY                              【人蛋白激酶B(PKB)ELISA試劑盒廠家】

9012596  Disposable Tips, 300µl (960)    Conducting disposable tips       CNY                             

133121     NeXtalStock Lithium citrate  (200)                  CNY                             

133119     NeXtalStock Ammonium citrate  (200)           CNY                             

133190     NeXtalStock D-(+)-Glucose (200)               CNY                             

51162       QIAamp 96 DNA Blood Kit (12)        For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels       CNY                             

50112       QIAamp UCP PurePathogen Blood Kit (10)               CNY                             

133104     NeXtalStock Sodium acet.  (200)            CNY                             

9015468  Sensor, photo, PM-L24              CNY                             

9001871  EZ1 Advanced, System     EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

9018458  Screw, w finishing washer (4), QX             CNY                             

9018447  Purge Tool, cartridge, QX                  CNY                             

9012412  Incubator Tower (65°) for Plato 3 hold              CNY                             

9012392  Bottle, waste, 10 l, BR8000              CNY                             

205111     Omniscript RT Kit (50)      For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

9018425  Door, sample, QX              CNY                             

9002123  QIAxcel Advanced System        QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                              【人蛋白激酶B(PKB)ELISA試劑盒廠家】

57714       QIAamp MinElute Virus Vacuum Kit (50)         For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)      CNY                             

972012     PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16 CNY                             

133227     NeXtalStock Sodium cacodyl. pH 6.0 (100)                CNY                             

301108     PolyFect Transfection Reagent (100 ml)  For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.      CNY                             

56304       QIAamp DNA Micro Kit (50)    For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)     CNY                             

9002031  Rotor-Gene Q MDx 5Plex HRM no PC               CNY                             

9002018  Rotor-Gene Q MDx 2plex HRM, Priority Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

970902     PyroMark Q24 Advanced Kit (4x24)                   CNY                             

133211     NeXtalStock Magnesium chloride H2O (200)            CNY                             

133195     NeXtalStock Sodium tartrate pH 5.6 (200)                CNY                             

QKT0640  α-L-巖藻糖苷酶測定試劑盒AFUPNPF-兩點法                                                 

QKT0641  α-L-巖藻糖苷酶測定試劑盒AFUCNPF-連續(xù)監(jiān)測法                                                 

QKT0642  α-淀粉酶測定試劑盒AMY   EPS連續(xù)檢測法                                           

QKT0643  血管緊張素化酶測定試劑盒ACE兩點法                                                   【人蛋白激酶B(PKB)ELISA試劑盒廠家】

QKT0644  乳酸脫氫酶測定試劑盒LDH-L      速率法                                         

QKT0645  肌酸磷酸激酶測定試劑盒CK速率法                                                

QKT0646  肌酸激酶同工酶測定試劑盒CK-MB速率法                                                      

QKT0647  超敏C-反應(yīng)蛋白測定試劑盒H-CRP免疫比濁法                                                       

QKT0648  α-羥基丁酸脫氫酶測定試劑盒α-HBDH速率法                                                      

QKT0649  D二聚體測定試劑盒D-Dimer免疫比濁法                                               

QKT0650  同型半胱氨酸測定試劑盒HCY酶循環(huán)法                                                  

QKT0651  二氧化碳測定試劑盒CO2     比色法                                         

QKT0652  鎂測定試劑盒Mg甲基百里香酚蘭法                                               

QKT0653  載脂蛋白測定試劑盒APoA1 免疫比濁法                                         

QKT0654  載脂蛋白B測定試劑盒ApoB        免疫比濁法                                         

QKT0655  高密度脂蛋白膽固醇測定試劑盒HDL-C      直接法                                         

QKT0656  總膽固醇測定試劑盒TC酶法                                                     

QKT0657  甘油三脂測定試劑盒TG酶法                                                    

QKT0658  低密度脂蛋白膽固醇測定試劑盒LDL-C直接法                                               

QKT0659  脂蛋白a測定試劑盒Lpa免疫比濁法                                                【人蛋白激酶B(PKB)ELISA試劑盒廠家】

QKT0660  胱抑素C測定試劑盒CysC     膠體金顆粒免疫比濁法                                              

QKT0661  N-乙酰-β-D-氨基葡萄糖苷酶測定試劑盒NAG兩點法                                                    

QKT0662  β2-微球蛋白測定試劑盒β2-MG免疫比濁法                                                 

QKT0663  肌酐測定試劑盒CRE酶法                                                  

QKT0664  尿素測定試劑盒Urea谷氨酸脫氫酶法                                                     

QKT0665  血清果糖胺測定試劑盒FA    終點法                                         

QKT0666  血糖測定試劑盒GLU     氧化酶法                                              

QKT0667  免疫球蛋白M測定試劑盒lgM免疫比濁法                                                      

QKT0668  白蛋白測定試劑盒Alb溴甲酚綠法                                                    

QKT0669  總蛋白測定試劑盒TP雙縮脲法                                                

QKT0670  血氨測定試劑盒AMM終點法