【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準(zhǔn)確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個完整的計劃。

【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

52304     QIAamp RNA Blood Mini Kit (50) For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers      CNY                           

51306     QIAamp DNA Mini Kit (250) For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)   CNY                           

9002012  Rotor-Gene Q MDx 2plex HRM Platform            CNY                           

970530    NRAS Pyro Kit (24)             CNY                           

970022    PyroMark Q24 CpG MLH1 (4 x 24)     For quantification of methylation level in region -209 to -188 from the transcription start site. CNY                           

969261    Ni-NTA Superflow 96 BioRobot Kit (4)        CNY                           

133199    NeXtalStock HEPES pH 8.0 (200)        CNY                            【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】

292143    mericon Horse Kit (24)         CNY                           

206047    Type-it Fast SNP Probe PCR Kit (4000)       "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"  CNY                           

292025    mericon Cattle (96)        CNY                           

291113    mericon RR Soy Kit (24)              CNY                           

9001908  QIAgility HEPA / UV (w/o PC) System QIAgility HEPA / UV (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.     CNY                           

133111    NeXtalStock Sodium fluoride (200)             CNY                           

9018540  Kit, installation, Qsym           CNY                           

9240672  Kerk Nut, Common, AP        CNY                           

9240658  Pump, Vacuum, Jun-Air, AP         CNY                           

9240642  Cable Assy, Proteinase K Pump, AP196/98          CNY                           

9240638  Opto Sensor, Reagent Pump, AP          CNY                           

9240617  Fuse, 2.0A, FNM, AP96        CNY                           

9240589  MAC Valve, 4-Way, Cent. Locator, AP96             CNY                           

9240560  Rotor/Buckets, Eppendorf, AP196/98          CNY                           

9240554  Bucket Grease, Eppendorf, AP196/98          CNY                           

9240523  Cable, Motor, Axis #0, Dispense, AP98        CNY                            【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】

9240507  Software & Std. Protocols, AP196              CNY                           

9240377  QIAcube, Installation     The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9239571  BR Universal, Basic Agreement     The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9239570  BR Universal, Premium Agreement       The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9020032  FOOTSET, RGQ           CNY                           

9019986  PCB, small breakout, 12 core, C12              CNY                           

9019479  UPPER BUFFER LID GS             CNY                           

9238864  BR M48, Premium Agreement       The Premium Cover Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9238852  LiquiChip, Basic Agreement   The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9241224  QIAxcel, Inspection Service  "Repair service delivery not applicable

Service response time (repair) not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: Yes, included"       CNY                           

NG0138  植物樣本提取液    100毫升 BR                       

NG0139  抗酸染液/Acid-faststain 50毫升×3     BR                       

NG0140  果糖測試盒    50管/48樣     比色法                        

NG0141  微量丙二醛測試盒/MDA測試盒 50管/24樣     比色法                         【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】

NG0142  細(xì)胞活性鑒別染液/Cellviabilitydetectedstain       20毫升   BR                       

NG0143  血管通透性測試染液/Vasopermeabilitystain 20毫升   BR                       

NG0144  過氧化物酶染液/Peroxidasestain  5毫升×3；2毫升×1  BR                       

NG0145  血清高密度脂蛋白膽固醇測試盒/HDL-C測試盒      80毫升   沉淀分離法手工                        

NG0146  血清低密度脂蛋白測試盒/LDL測試盒      80毫升   手工/半自動                       

NG0147  蔗糖測試盒    50管/48樣     比色法                        

NG0148  蔗糖磷酸合成酶測試盒/SPS測試盒   50管/24樣     比色法                        

NG0149  蔗糖合成酶測試盒/SS測試盒     50管/24樣     比色法                        

NG0150  硝酸還原酶測試盒/NR測試盒    50管/24樣     比色法                        

NG0151  ATP含量測試盒    100管/48樣   比色法                        

NG0152  γ-谷氨酰胺半胱氨酸合成酶測試盒/γ-GCS測試盒       100管/48樣   微量定磷法                        

NG0153  F0F1-ATP酶/ATP合成酶測試盒 20管/10樣     生化法                        

NG0154  二胺氧化酶測試盒/DAO測試盒  25毫升/40T   手工比色法                        

NG0155  質(zhì)粒提取試劑盒    100T/套  BR                       

NG0156  紅細(xì)胞裂解液       100毫升 BR                       

NG0157  RIPA組織細(xì)胞快速裂解液  20毫升   BR                        【人糖原合成酶激酶(GSK)ELISA試劑盒現(xiàn)貨】

NG0158  血糖測試盒/葡萄糖測試盒   96T 酶法                           

NG0159  丙氨酸氨基轉(zhuǎn)移酶測試盒    60T PO法                         

NG0160  蛋白質(zhì)羧基測試盒/蛋白質(zhì)羰基測試盒      50管/24樣     紫外比色法                        

NG0161  過氧化氫測試盒/H2O2測試盒    50管/48樣     比色法                        

NG0162  銅蘭蛋白測試盒/銅藍蛋白測試盒/CP測試盒    50管/48樣     比色法                        

NG0163  抗“O”膠乳測試盒     100T×2 BR                       

NG0164  類風(fēng)濕因子膠乳試劑盒       100T×2 BR                       

NG0165  血氨測試盒    50管/48樣     比色法                        

NG0166  乙醇脫氫酶測試盒       50管/48樣     紫外比色法                        

NG0167  還原型谷胱甘肽+巰基測試盒/GSH+-SH測試盒       100管/48樣   比色法                        

NG0168  DAB濃縮型試劑盒       3毫升     BR