1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

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9242712  Validation Support, 2d QIAcube       The QIAGEN QIAcube Guided Validation includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)      CNY                             

9019226  Fan - rear 92x92, PyroQ24                 CNY                             

9019220  PCB - Distribution robot, PyroQ96MD               CNY                             

1066843  PG Influenza A RT-PCR Assay (48) LE Z              CNY                             

1066848  NDV Univ. RT-PCR REAGENT LE                 CNY                             

1066854  AIV-H9 RT-PCR REAGENT LE             CNY                             

1060992  Buffer MW1 (77ml)           CNY                             

1061059  Sample Preparation Reagents, 8ml                   CNY                             

1061080  NG Quantification Standards            CNY                             

1061113  PG AIV-H7 RT-PCR Assay (48) Z                  CNY                             

9016568  Plate Grippers, RCS1                  CNY                              【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】

9016536  Transportation box , BioSprint 15             CNY                             

9016535  Transportation box , Twister II                   CNY                             

9016533  Transportation box , RapidPlate onlf.                CNY                             

9016530  Transportation box 1, LiquiChip                 CNY                             

9016493  BR MDx DSP Service, PRO                 CNY                             

9016325  Washer head with holder , P 13xx            CNY                             

9019185  PCB - Front, PyroQ96MD          CNY                             

9011902  Upgrade, tip change system             CNY                             

9011824  Z-Modul; Tip-Adapter 1100µl; BR8000            CNY                             

9237033  Cable, Liquid Sensor, BR8 RD v1.0            CNY                             

9236960  Probe Holder, Body, BR8 RD             CNY                             

9019144  Fan - rear 92x92, PyroQ96MD                   CNY                             

9019113  PCB - Camera, PyroQ96ID                 CNY                             

9019075  PyroMark NDT Holder, PyroQ96MD        Re-usable holder for tips, Nucleotide Dispensing Tips ａnd Reagent Dispensing Tips, for use in PyroMark Q96 MD.  CNY                             

9019010  Motor M, EZ1xl                 CNY                             

9018953  Reagent Block, 16x0.2ml/2ml PCR/Flat-Bas              CNY                              【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】

9018938  Adapter, Light Cycler 480 384-Well Plate                  CNY                             

9018935  Adapter, reagent tube SBS Plate              CNY                             

9015837  Upg. Kit, Y6L Connection, BR8 (ISI21)               CNY                             

9015689  Fittings (10), M6/3.3 mm, white                CNY                             

9015605  Upgrade, LiquiChip IS 2.x, PLUS                 CNY                             

QY-x2372 人胚肺細(xì)胞WI-38                                               

QY-x2373 非洲綠猴腎細(xì)胞Vero                                                  

QY-x2374 細(xì)胞淋巴瘤細(xì)胞U-937                                               

QY-x2375 人腦星形膠質(zhì)母細(xì)胞瘤U-87MG                                               

QY-x2376 人膠質(zhì)瘤細(xì)胞U251                                                     

QY-x2377 人骨肉瘤細(xì)胞U-2OS                                                    

QY-x2378 單核細(xì)胞系THP-1                                               

QY-x2379 人血液白血病細(xì)胞TF-1                                                       

QY-x2380 人舌鱗癌細(xì)胞Tca-8113                                              

QY-x2381 蝙蝠肺細(xì)胞Tb1Lu                                               

QY-x2382 人乳腺管癌細(xì)胞T-47D                                                

QY-x2383 人膀胱移行細(xì)胞癌細(xì)胞T-24                                                       

QY-x2384 人結(jié)腸癌細(xì)胞SW620                                                  

QY-x2385 人甲狀腺鱗癌細(xì)胞SW579                                                  

QY-x2386 人結(jié)腸癌細(xì)胞SW[SW-]                                                 【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】

QY-x2387 豬睪丸細(xì)胞系ST                                                  

QY-x2388 小鼠骨髓瘤細(xì)胞SP2/0                                                 

QY-x2389 人肝癌細(xì)胞SMMC-7721                                                      

QY-x2390 人卵巢癌細(xì)胞SK-OV-3                                                 

QY-x2391 人肝癌細(xì)胞SK-HEP-1                                                   

QY-x2392 人乳腺腺癌細(xì)胞SK-BR-3                                                     

QY-x2393 人子宮頸鱗癌細(xì)胞SiHa                                                       

QY-x2394 人膠質(zhì)瘤細(xì)胞SHG-44                                                 

QY-x2395 人胃癌SGC7901                                                   

QY-x2396 人成骨肉瘤細(xì)胞Saos-2                                               

QY-x2397 大鼠雪旺細(xì)胞RSC96                                                    

QY-x2398 小鼠前列腺癌細(xì)胞RM-1                                                     

QY-x2399 人結(jié)腸腺癌細(xì)胞RKO                                                   

QY-x2400 猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞RF/6A                                                     

QY-x2401 小鼠單核巨噬細(xì)胞白血病細(xì)胞RAW264.7                                                

QY-x2402 人Burkitt＇s淋巴瘤細(xì)胞Raji                                                 【人精氨酸酶(Arg)ELISA試劑盒現(xiàn)貨】