1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！【人胰激肽原酶(PK)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人胰激肽原酶(PK)ELISA試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人胰激肽原酶(PK)ELISA試劑盒現(xiàn)貨】ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標記物如酶標結(jié)合物則通過各種化學合成方法制備.近年來,隨著分子生物學的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【人胰激肽原酶(PK)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

9240740 Tubing, Tygon, 1/4" ID      CNY            

9240735 Tubing, Black, Capper, AP196/98     CNY            

9240724 Tube Assy, Draw, AP96/196       CNY            

5000-1412   Poteneiometer 500K, VTX2        CNY            

9240506 Monitor, 17", Flat Panel, AP        CNY            

9239851 Cover, LC200        CNY            

9239565 xMAP Alignment Microspheres     CNY            

9019942 Barrel removal tool, CX1        CNY             【人胰激肽原酶(PK)ELISA試劑盒現(xiàn)貨】

9019474 Lid, UV, polycarb, C1x      CNY            

9238884 Stepper motor, turntable, BS96      CNY            

9238866 BR MDx, Premium Agreement   The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).  CNY            

9238857 Basic Cover Agreement Plato3, 1yr   The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9238838 #Twister I, Basic Agreement, no PM  The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9241220 QIAxcel, Installation   "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.

This installation covers the handling of the QIAxcel systems hardware ａnd familiarize with the basic instrument ａnd software functions of the BioCalculator software. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements." CNY            

9241195 Cable, RS232, Belkin F2N209-06-T, 1.8m      CNY            

9241186 Sensor, interlock2, with cable, QX      CNY            

9238831 BR 8000, Basic Agreement    The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9238822 Rect. carrier for 1 tube 100 ml     CNY            

9237194 Reservoir RapidPlate        CNY            

9237171 Heater Block XYP        CNY            

9233168 Mixer Board     CNY            

9237154 Drive Mechanism XYP     CNY            

9237110 Upgrade App., QIAamp 96 DNA Blood   On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Blood including kit   CNY            

9237095 Training, HiLight On-site   On-site Training 1 day HiLight  CNY             【人胰激肽原酶(PK)ELISA試劑盒現(xiàn)貨】

9237084 Installation, BR plus 3 integrations        CNY            

9236414 Plate Stacker TW II     CNY            

9232973 Level Detector Board P7-8 channels      CNY            

9232954 LSW Board (Level sensor waste)      CNY            

9232921 Comparator board 1.02 w/clot detection      CNY            

9236337 Digital Board       CNY            

9236155 Tool, calibration, syringe, RapidPlate      CNY            

9235702 Cables, Shaker, RCS1        CNY            

9235626 Lead Screw Nut, MP Holder LH, Shaker8000        CNY            

QY-x2000    U266 骨髓瘤 1640+10%胎牛血清                       

QY-x2001    BIU87 膀胱癌 1640+10%胎牛血清                      

QY-x2002    SHG-44 腦惡性膠質(zhì)瘤 1640+10%胎牛血清                        【人胰激肽原酶(PK)ELISA試劑盒現(xiàn)貨】

QY-x2003    U251 神經(jīng)膠質(zhì)細胞瘤 1640+10%胎牛血清                       

QY-x2004    M17 神經(jīng)母細胞瘤 DMEM+10%胎牛血清                      

QY-x2005    LAN-5 神經(jīng)母細胞瘤 1640+10%胎牛血清                    

QY-x2006    LAN-6 神經(jīng)母細胞瘤 1640+10%胎牛血清                    

QY-x2007    SK-N-SH 神經(jīng)母細胞瘤 DMEM+10%胎牛血清                      

QY-x2008    SH-SY5Y 神經(jīng)母細胞瘤 DMEM+10%胎牛血清                      

QY-x2009    SF126 腦瘤 1640+10%胎牛血清                    

QY-x2010    SF17 腦瘤 1640+10%胎牛血清                     

QY-x2011    SF763 腦瘤 瘤株                    

QY-x2012    SF767 腦瘤 瘤株                    

QY-x2013    MLA-144 長臂猿淋巴瘤 1640+10%胎牛血清                      

QY-x2014    T47D 乳腺導管癌 1640+15%胎牛血清                       

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QY-x2019    Jurkat 急性T淋巴細胞白血病 1640+10%胎牛血清                       

QY-x2020    U937 單核細胞白血病 1640+10%胎牛血清                       

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QY-x2022    CEM 白血病 1640+10%胎牛血清                    

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QY-x2027    SK-BR-3 乳腺腺癌 1640+15%胎牛血清                      

QY-x2028    MCF-7 乳腺腺癌 1640+10%胎牛血清                    

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