【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

本試劑盒僅供研究使用，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話(huà)也可以用一下生理鹽水，還是用PBS

【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問(wèn)題請(qǐng)來(lái)電，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

286115    cador N. caninum PCR Reagent (96)           CNY                           

282603    virotype Influenza A RT-PCR Kit (24)          CNY                           

282607    virotype Influenza A RT-PCR Kit (480)        CNY                           

280373    virotype BVDV RT-PCR Kit (24)          CNY                           

280445    virotype BTV pan/8 RT-PCR Kit (96)           CNY                           

9018703  EZ1 Adv. XL DSP Virus Card        CNY                           

9001252  BIOROBOT 8000 Platf.Promo,Reaction Setup            CNY                           

9019515  ZLV Y-piece, pinch valve tubing, CX1          CNY                           

9020575  LED Assy, Green, 15" Long, DML 3000             CNY                           

9020593  Lid, DML3000              CNY                            【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】

9022383  Original Transport Box, QXA              CNY                           

9013308  Base, vacuum manifold, v1.04             CNY                           

9013298  Top plate, vacuum manifold96 plate , 4        CNY                           

9013236  Tubing set, vacuum, 200, 0          CNY                           

9013224  Gripper set, V-handler, BR3000            CNY                           

9013197  Tubing, L1 outlet, auto vac,BR8000 v1.00          CNY                           

9011702  Cable, PCON98 to AC-box (level sensor),           CNY                           

9011681  Dispenser drive, BR8000              CNY                           

132635    NeXtal Stock Zinc chloride (50)    0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)     CNY                           

132578    NeXtal Stock PEG 2,000 DME (50)     50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)       CNY                           

800341    Cosmid End-Sequencing, w/ prep        CNY                           

800308    siRNA Hairpin Run w/ prep          CNY                           

800121    Verification Sequencing, w/ prep   "Double-stranded sequencing of a given sequence

QIAGEN® DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99.99%"      CNY                           

132528    NeXtal Stock CAPSO untit. (50)   0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)   CNY                           

132510    NeXtal Stock ADA untit. (50)       0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)       CNY                           

764114    PAXgene Bone Marrow RNA Tubes (50)            CNY                            【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】

679913    ipsogen RT Kit (33)              CNY                           

203643    HotStarTaq Plus Master Mix Kit (250)  "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."     CNY                           

201225    Taq PCR Core Kit (1000)      "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

201203    Taq DNA Polymerase (250)   "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

180922    GeneRead Adapter L Set 1plex (12)             CNY                           

180912    GeneRead Adapter I Set 1plex (12)              CNY                           

159214    QIAcard FTA Indicator Four Spots (25)             CNY                           

QY-x1938       ZR-75-1 乳腺導(dǎo)管癌 SF767 腦瘤                                       【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】

QY-x1939       A10大鼠主動(dòng)脈平滑肌                                         

QY-x1940       人主動(dòng)脈平滑肌　CNE-1 高分化鼻咽鱗癌 1640+10%胎牛血清                                    

QY-x1941       CNE-2 低分化鼻咽鱗癌 1640+10%胎牛血清                                     

QY-x1942       KB 口腔表皮樣癌 1640+10%胎牛血清                                       

QY-x1943       Hep-2 喉癌 1640+10%胎牛血清                                   

QY-x1944       Ec109 食管癌 1640+10%胎牛血清                                      

QY-x1945       KYSE 450 食管癌 1640+10%胎牛血清                                       

QY-x1946       KYSE 150 食管鱗癌 1640+10%胎牛血清                                   

QY-x1947       EC97076 食管癌 1640+10%胎牛血清                                         

QY-x1948       BGC-823 胃腺癌 1640+10%胎牛血清                                        

QY-x1949       MGC-803 胃腺癌 1640+10%胎牛血清                                       

QY-x1950       SGC7901 胃低分化腺癌 1640+10%胎牛血清(P53基因第六外顯子204位密碼發(fā)生缺失突變)                                   

QY-x1951       MKN-45 低分化胃癌 1640+10%胎牛血清                                         

QY-x1952       HuTu-80 十二指腸腺癌 MEM+非必需氨基酸+10%胎牛血清                                         

QY-x1953       Caco-2 結(jié)腸癌 MEM+非必需氨基酸+10%胎牛血清                                        

QY-x1954       COLO 205 結(jié)腸癌 1640+10%胎牛血清                                       【人組織蛋白酶D(cath-D)ELISA試劑盒現(xiàn)貨】

QY-x1955       COLO320DM 結(jié)腸癌 1640+10%胎牛血清                                         

QY-x1956       HCT-8 回盲腸腺癌 1640+10%胎牛血清                                     

QY-x1957       HCT-116 低分化結(jié)腸腺癌 1640+10%胎牛血清                                         

QY-x1958       HT-29 結(jié)腸腺癌 1640+10%胎牛血清                                         

QY-x1959       LS-174T 結(jié)腸腺癌 MEM+非必需氨基酸+10%胎牛血清                                         

QY-x1960       SW480 結(jié)腸癌 L15                                      

QY-x1961       BEL-7402 肝細(xì)胞癌 1640+10%胎牛血清                                    

QY-x1962       BEL-7405 肝細(xì)胞癌 1640+10%胎牛血清                                    

QY-x1963       H7402 肝癌 1640+10%胎牛血清                                         

QY-x1964       HCC-9204 肝癌 1640+10%胎牛血清                                   

QY-x1965       Hep3B 肝癌 DMEM+非必需氨基酸+10%胎牛血清                                   

QY-x1966       Hep G2 肝癌 DMEM+10%胎牛血清                                    

QY-x1967       SMMC-7721 肝癌 1640+10%胎牛血清                                       

QY-x1968       PANC-1 胰腺導(dǎo)管上皮癌 1640+10%胎牛血清