1.購買我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測(cè)，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請(qǐng)勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測(cè)標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲(chǔ)。 【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測(cè)稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號(hào)的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開始之前，清潔工作臺(tái)。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測(cè)，對(duì)收集后當(dāng)天就進(jìn)行檢測(cè)的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請(qǐng)?jiān)炷Ｈ〔暮�，將�?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測(cè)標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請(qǐng)?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測(cè)指標(biāo)及其它特殊要求。

【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】五、代測(cè)數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

9238858  Basic Cover Agreement Plato7, 1yr          The Basic Cover Agreement Plato7 provides full service coverage for the Plato 7 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9241233  User Manual, QIAxcel FA 01/08 (WW)                CNY                             

9241227  ZLV, Handscanner, Qsym          CNY                             

9241180  Motor, Z, QX             CNY                             

9019473  Loading guide, 52 well sharkstooth, GSC                   CNY                             

9019462  Switch, micro, HS              CNY                             

9019457  Fans (2), 5V, 30mm, HS             CNY                             

9237172  Cable USB                  CNY                             

9233169  Board, YZ-CPU, BR8000            CNY                              【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】

9237163  Bezel LiquiChip                   CNY                             

9237162  Shipping Plug XYP              CNY                             

9237135  #Twister I, Full Agreement, no PM The Full Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                             

9237109  Protocol Dev./Opt., on-site (5-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237102  Upgrade, BR plus 2 ext. devices       Upgrade BioRobot with two approved integration devices    CNY                             

9232974  Card, x-main, peristaltic XPUPG                CNY                             

9232972  YZ 96 CPU Board/Driver Unit            CNY                             

9232947  Springcontact, RoboVac, BR8000             CNY                             

9236408  Storage Platform TW I, deep well             CNY                             

9232918  Board, PCON, auto vac, BR8000               CNY                             

9236334  Green LSR                  CNY                             

9236324  Fan, LiquiChip            CNY                             

9236151  Locator Plate, 96 well,pos 2,BR RP          CNY                             

9230820  Rack for reagents, Universal (zig-zag)              CNY                             

9230718  Predilution-Rack 320x2ml Rose-80           CNY                             

9230365  Cable,Interface,flat,(Syr.)-Incub.-Read.            CNY                              【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】

9021284  Safety lock, waste drawer, v1, Qsym                 CNY                             

9238611  Rubber seal, centrifuge motor                  CNY                             

9238477  Tubing, OLF pump head, RapidPlate                  CNY                             

9238245  Tray for Reaction               CNY                             

9238191  Upgrade App., DirectPrep 96           CNY                             

9237721  EZ1, Full Agreement         The Full Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).   CNY                            

9240676  Bimba Cylinder, Latch Lock, AP98             CNY                             

9240668  Nut, Flangeless,1/4-28, Blue, AP                CNY                             

QY-x1014 人肝癌細(xì)胞HepG2/2-15                                              

QY-x1015 人肝癌細(xì)胞9204                                                  【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】

QY-x1016 人肝癌細(xì)胞HuH-7                                               

QY-x1017 人肝癌細(xì)胞FL62891                                                    

QY-x1018 人膽管細(xì)胞型肝癌細(xì)胞HCCC-9810                                                    

QY-x1019 人肝癌細(xì)胞HCC-9724                                                  

QY-x1020 人肝癌細(xì)胞HHCC                                                

QY-x1021 人肝癌細(xì)胞系Hep-3B                                                 

QY-x1022 表達(dá)HBV病毒的人肝細(xì)胞HepG2.2.15                                                      

QY-x1023 人肝癌細(xì)胞H7402                                              

QY-x1024 人胚肝二倍體細(xì)胞CCC-HEL-1                                                     

QY-x1025 人肝細(xì)胞FL62891                                                

QY-x1026 人感染HCV肝癌細(xì)胞HuH7-HCV                                               

QY-x1027 永生化鼻咽上皮細(xì)胞系NP69-SV40T                                                  

QY-x1028 人鼻咽癌母系細(xì)胞CNE-2Z                                                  

QY-x1029 人鼻咽癌細(xì)胞CNE                                              

QY-x1030 人鼻咽癌細(xì)胞（高分化）CNE1                                                  

QY-x1031 人鼻咽癌細(xì)胞（低分化）CNE2                                                   【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒廠家】

QY-x1032 人鼻咽癌細(xì)胞HNE1                                                     

QY-x1033 人鼻咽癌細(xì)胞HNE2                                                     

QY-x1034 人鼻咽癌細(xì)胞HNE3                                                     

QY-x1035 人鼻咽癌細(xì)胞HONE1                                                  

QY-x1036 轉(zhuǎn)基因鼻咽癌細(xì)胞系CNE1-lmp\l                                               

QY-x1037 低分化鼻咽癌細(xì)胞系SUNE-1                                                      

QY-x1038 鼻咽癌細(xì)胞系SUNE-1亞株-5-8F                                                

QY-x1039 5-8轉(zhuǎn)基因鼻咽癌細(xì)胞系F2-2                                                     

QY-x1040 人鼻咽癌細(xì)胞系SUNE-1亞株6-10B                                                  

QY-x1041 人鼻咽癌細(xì)胞系SUNE-1亞株13-9B                                                  

QY-x1042 人鼻咽癌細(xì)胞系HNE2-LMP1/2                                                    

QY-x1043 人鼻咽癌細(xì)胞系HNE2-LMP1                                                       

QY-x1044 人鼻咽癌細(xì)胞系SUME-a