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1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測測定的精度和提高檢測結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個工具都有一個包含一個典型數(shù)據(jù)的圖表的手冊。在運(yùn)營商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應(yīng)該如何儲存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長期儲存，建議凍結(jié)他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會給出錯誤的結(jié)果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經(jīng)檢驗的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動態(tài)范圍內(nèi)的測定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測的。

9。我每次都要運(yùn)行一個空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當(dāng)排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進(jìn)行質(zhì)量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來很好，但我沒有得到一個信號，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請檢查是否正確進(jìn)行稀釋。過稀釋會導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動洗衣機(jī)也是一樣的。一個中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】

14。我需要用一個盤子嗎？

可靠的結(jié)果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細(xì)胞來源。這個信息的*佳來源是目前的文獻(xiàn)，很容易通過互聯(lián)網(wǎng)在多個科學(xué)數(shù)據(jù)庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9019565  Clamp, cable 2 wires 2 tabs, Cxx               CNY                             

9019543  Bow spring, table rail, Cxx                 CNY                             

9019510  CHAMBER MAIN VAC STATION 1820                 CNY                             

9019505  PLATE SEPARATOR 48 WELL HIGH            CNY                             

204256     QuantiFast Probe PCR Kit (2000)               CNY                             

204174     Rotor-Gene SYBR Green RT-PCR Kit (400)       The Rotor-Gene SYBR Green RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time one-step RT-PCR using SYBR Green I.   CNY                             

204154     QuantiFast SYBR Green RT-PCR Kit (400)                  CNY                             

9019424  Wiring, lower heater, RG3                CNY                             

9019417  Fan, lid, RG3              CNY                             

9019393  Solenoid, cooling with flap, RG3                CNY                             

9019386  Photo Multiplier Tube with cable, RG3             CNY                             

9019368  Damper, lid, RG6               CNY                              【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】

9019347  Motor, 12VDC Heater fan, RG6                CNY                             

9234697  Manual Hardware Plato 7                 CNY                             

9020477  Gasket, front plate, GSC           CNY                             

9234463  Coupling, CPC, inline, female, 8mm                   CNY                             

1027285  AllStars Neg. siRNA AF 546 (5 nmol)                  CNY                             

1022564  Mm/Hs_MAPK1 control siRNA (5 nmol)           CNY                             

9019205  Opto - Stacker, PyroQ96MD              CNY                             

9019204  Opto - Robot Z motor , PyroQ96MD                  CNY                             

9019200  Hinge kit - Top cover, PyroQ96MD            CNY                             

1022073  Luciferase GL3 siRNA (5 nm)             CNY                             

1022064  GFP-22 siRNA (5 nm)                  CNY                             

1018996  PCR Buffer, 10 x (22ml/30),KG           CNY                             

1064605  MagAttract M48 DNA Manual Kit  (200)                CNY                             

1066851  PG FMDV RT-PCR Assay (48) LE Z              CNY                             

1061054  HBV PCR REAGENT I                  CNY                             

1061069  UU PCR REAGENT II                   CNY                              【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】

1061079  NG PCR REAGENT II                   CNY                             

9019886  Lid, label 4CH, RG3           CNY                             

9019875  Cover, main, silver, RGQ           CNY                             

9019836  IPC Hard Drive PCA6751,  PyroQ96ID             CNY                             

9019769  Valve, shutoff, V2, QX                CNY                             

QY-AC062          小鼠白血病細(xì)胞 L1210          L1210                                            

QY-AC063          大鼠主動脈平滑肌細(xì)胞 A7r5       A7r5                                              

QY-AC064          大鼠肝細(xì)胞瘤細(xì)胞 H-4-II-E  H-4-II-E                                         

QY-AC065          小鼠結(jié)腸細(xì)胞 CT26       CT26                                              

QY-AC067          小鼠骨髓瘤細(xì)胞 SP2/0-S        SP2/0-S                                          

QY-AC069          小鼠胰島內(nèi)皮細(xì)胞 MS1        MS1                                     

QY-AC070          小鼠骨髓瘤細(xì)胞 NS-1P3/NSI/1-Ag4-1   NS-1P3/NSI/1-Ag4-1                                     

QY-AC071          小鼠逆轉(zhuǎn)錄病毒包裝株 PT-67      PT-67                                             

QY-AC072          小鼠肺上皮細(xì)胞 TC-1   TC-1                                     

QY-AC073          大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞高分化 PC-12Well-differentiated PC-12Well-differentiated                                     

QY-AC074          大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞未分化 PC-12Undifferentiated       PC-12Undifferentiated                                         

QY-AC075          小鼠腎小球系膜細(xì)胞 SV40-MES-13     SV40-MES-13                                       

QY-AC076          小鼠Lewis肺癌細(xì)胞 LLC       LLC                                       

QY-AC077          中國倉鼠卵巢懸浮細(xì)胞biohermes CHO-S    CHO-S                                           

QY-AC078          中國倉鼠卵巢細(xì)胞invitrogen CHO-S     CHO-S                                            【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】

QY-AC079          倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷 CHO/dhFr-         CHO/dhFr-                                             

QY-AC080          小鼠淋巴瘤細(xì)胞 L5178Y TK+/- clone 3.7.2C          L5178YTK+/-clone3.7.2C                                     

QY-AC081          小鼠乳腺癌高轉(zhuǎn)移細(xì)胞 MA-891 MA-891                                        

QY-AC082          小鼠宮頸癌細(xì)胞 U14    U14                                      

QY-AC083          小鼠胰島素瘤胰島β細(xì)胞 Beta-TC-6  Beta-TC-6                                              

QY-AC084          小鼠睪丸間質(zhì)細(xì)胞 TM3        TM3                                     

QY-AC085          猴腎細(xì)胞 Marc145         Marc145CRL-12231                                              

QY-AC086          小鼠白血病細(xì)胞 P388  P388                                              

QY-AC087          大鼠成骨細(xì)胞 ROS1728         ROS1728                                      

QY-AC088          非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞 COS-7   COS-7                                            

QY-AC089          小鼠胚胎細(xì)胞 NIH/3T3 NIH/3T3                                        

QY-AC090          小鼠黑色素瘤細(xì)胞 B16F1     B16F1                                           

QY-AC091          大鼠腎細(xì)胞 NRK-52E     NRK-52E                                       

QY-AC093          鼠肝星形細(xì)胞 CFSC-8B CFSC-8B                                        

QY-AC094          鼠肝星形細(xì)胞 CFSC-2G         CFSC-2G                                       

QY-AC095          人/鼠雜交骨髓瘤細(xì)胞 K6H6/B5   K6H6/B5                                        【人血管緊張肽酶A(ATA)ELISA試劑盒現(xiàn)貨】