【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】規(guī)格：48T/96T

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

本試劑盒僅供研究使用，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話也可以用一下生理鹽水，還是用PBS

【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問(wèn)題請(qǐng)來(lái)電，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9013262  Cable, 24 V, power to power, 1200 mm              CNY                           

9013249  BGR Wash System        CNY                           

9013207  Service kit, accessories, BR8000          CNY                           

9013194  Tubing, L0 outlet, auto vac,BR8000 v1.00          CNY                           

9013187  Holder, retractable tubing, dispenser, B         CNY                           

800328    Miniprep, PCR purification (Easy-Read)              CNY                           

132109    EasyXtal 15-Well DG-Tool X-Seal (100)      "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."   CNY                           

132101    NeXtal DWBlock Adhesive Foils          CNY                           

132045    NeXtal Evolution µplate (10)         CNY                           

338172    RT2 Calibration Plate A         CNY                           

338125    384EZload Covers (4)           CNY                           

762431    PAXgene Blood RNA MDx Kit (4)        CNY                           

203745    QIAGEN Fast Cycling PCR Kit (1000) For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution    CNY                           

203609    HotStarTaq Plus DNA Polymerase (25000)   100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2 CNY                           

201205    Taq DNA Polymerase (1000) "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."      CNY                           

180850    GeneRead qPCR FAST SYBR Green/ROX MM (2)            CNY                            【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】

159300    QIAcard FTA Purification Reagent (500ml)         CNY                           

158928    Lytic Enzyme Solution (650 µl)            CNY                           

9240549  Imbalance PCB, Eppendorf, AP196/98         CNY                           

9240547  Motor, 230V, Eppendorf, AP196/98             CNY                           

9240528  Cable, Motor, Axis #5, TR, AP98         CNY                           

9240047  Thermostate unit, 120V, 1.04        CNY                           

9020029  Cable RC-3034, RGQ           CNY                           

9020017  Oil for PM, EZ1            CNY                           

9020016  Cable harness, VacuumStation PCB, CX1            CNY                           

9019938  Opto switch, P-axis, 1 ch. , Cx2          CNY                           

9019486  HEPA filter, replacement cartridge, Cxx        CNY                           

9238861  BR 8000, Premium Agreement      The Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9238855  BR MDx, Basic Agreement    The Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9238850  EZ1, Basic Agreement    The Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY                           

9241226  Barcode Reader, EZ1a           CNY                           

9238812  Rectangular carrier, undrilled        CNY                            【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】

9019467  PLATE GLASS SHORT SET GS          CNY                           

QY-x1752       人骨肉瘤細(xì)胞       SW1353                             

QY-x1753       人白血病細(xì)胞       Daudi                                 

QY-x1754       轉(zhuǎn)染了tk基因的SW038-C2細(xì)胞       SW038-C2-tk                            

QY-x1755       人星形膠質(zhì)細(xì)胞瘤細(xì)胞      SHC-44                              

QY-x1756       人神經(jīng)膠質(zhì)瘤細(xì)胞       BT-325                               

QY-x1757       人XG惡性膠質(zhì)瘤細(xì)胞 SF-295                               

QY-x1758       人神經(jīng)母細(xì)胞瘤細(xì)胞    SK-N-SH                            

QY-x1759       人宮頸癌細(xì)胞       Hela                            

QY-x1760       人宮頸癌細(xì)胞       SiHa                            

QY-x1761       人乳腺腺癌細(xì)胞    MCF-7                              

2QY-x1762     人膀胱變移細(xì)胞癌細(xì)胞       T-24                                   【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】

QY-x1763       人纖維肉瘤細(xì)胞    HT-1080                             

QY-x1764       人肝靜脈內(nèi)皮細(xì)胞       ED-25                                

QY-x1765       腸系膜下腔動(dòng)脈血管內(nèi)皮細(xì)胞    Ealy926                              

QY-x1766       人視網(wǎng)膜色素上皮細(xì)胞       D407                                 

QY-x1767       人胚肺成纖維細(xì)胞       HLF                                  

QY-x1768       人肝星狀細(xì)胞       HSC                                  

QY-x1769       人正常胎肝細(xì)胞    L-o2                                  

QY-x1770       逆轉(zhuǎn)錄病毒包裝用的人胚胎成纖維細(xì)胞    PA317                                

QY-x1771       轉(zhuǎn)染入tk基因的PA317細(xì)胞      PA317-tk                                 

QY-x1772       轉(zhuǎn)染入GFP基因的PA317細(xì)胞（GFP-綠色熒光蛋白）  PA317-GFP                               

QY-x1773       人胚腎細(xì)胞    HEK-293                            

QY-x1774       人CD4+T淋巴細(xì)胞     H9                              

QY-x1775       胎兒骨髓間充質(zhì)細(xì)胞（自建）    FBM                                  

QY-x1776       　    人腦細(xì)胞                                  

QY-x1777       人腦膠質(zhì)細(xì)胞株    HEB                            

QY-x1778       總T淋巴細(xì)胞       OKT3                                 【人鳥(niǎo)氨酸氨基甲酰轉(zhuǎn)移酶(OCT)ELISA試劑盒現(xiàn)貨】

QY-x1779       BALB/C小鼠肝癌細(xì)胞  H22                            

QY-x1780       小鼠前列腺癌細(xì)胞       RM-1                                 

QY-x1781       大鼠肝癌細(xì)胞（wistar 大鼠）    CBRH7919                                

QY-x1782       小鼠肝癌細(xì)胞       HePa1-6