檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】實驗原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】標本要求：

1．標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設(shè)標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時藍色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進行.

注意事項：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果. 【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復(fù)孔.如標本中待測物質(zhì)含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據(jù)樣品的OD     

值由標準曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

QY-07700 SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A(抗原)                   0.5mg/6501mg/1100                                  

QY-06790 SYVN1(synovial apoptosis inhibitor 1)滑膜細胞凋亡抑制物1（抗體）                  0.5mg/6501mg/1100                                  

QY-06850 SYK (Spleen tyrosine kinase)非受體型酪氨酸激酶(抗原)                 0.5mg/6501mg/1100                                  

QY-04190 Tau protein微管相關(guān)蛋白（抗原）                0.5mg/6501mg/1100                                  

QY-06380 TGF-βR 1/ALK-5(TGF-beta receptor type I)轉(zhuǎn)移生長因子–β受體 1（抗原）                   0.5mg/6501mg/1100                                  

QY-07780 Thy-1/CD90CD90(抗原)              0.5mg/6501mg/1100                                  

QY-06320 Thioster-containing protein 1硫酯包含蛋白-1(抗原)              0.5mg/6501mg/1100                                  

QY-06400 TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )P53誘導(dǎo)糖酵解和凋亡調(diào)節(jié)因子蛋白（抗原）            0.5mg/6501mg/1100                                   【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】

QY-04180 TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金屬蛋白酶組織抑制因子-4(抗原)             0.5mg/6501mg/1100                                  

QY-04470 TPO(Thyroid Peroxidase)甲狀腺過氧化物酶(抗原)                  0.5mg/6501mg/1100                                  

QY-04600 TSHR (Thyroid stimulating hormone receptor)促甲狀腺素受體（抗原）              0.5mg/6501mg/1100                                  

QY-04490 TSLP(Thymic stromal lymphopoietin isoform 1)淋巴細胞生成素-1（抗原）                   0.5mg/6501mg/1100                                 

QY-04460 TTF-2(thyroid transcription factor 2;forkhead box E1)甲狀腺轉(zhuǎn)錄因子-2(抗原)             0.5ml/6501mg/1100                                   

QY-08190 Tyrosinase酪氨酸酶(多肽抗原)               0.5mg/6501mg/1100                                  

QY-06030 OAT4L又稱URAT1 (Urate Transporter 1)尿酸鹽重吸收轉(zhuǎn)運子1 (抗原)               0.5mg/6501mg/1100                                  

QY-08530 VAP1(vascular adhesion protein 1)血管粘附蛋白1（多肽抗原）                  0.5mg/6501mg/1100                                  

QY-06590 VCAM-1(Vascuolar cell adhesion molecule 1)CD106血管內(nèi)皮細胞粘附分子(抗原)               0.5mg/6501mg/1100                                  

QY-07560 Vimentin波形蛋白（抗原）                    0.5mg/6501mg/1100                                  

QY-04790 VSIG4 (V-set ａnd immunoglobulin domain-containing protein 4)VSIG4 T淋巴細胞負調(diào)節(jié)蛋白（抗原）            0.5mg/6501mg/1100                                  

QY-04290 WNK4(Ser/Thr-protein kinase WNK4; protein kinase with no lysine 4; protein kinase, lysine –dficient 4)一種新的絲氨酸/蘇氨酸激酶家族成員的基因WNK4（多肽抗原）                0.5mg/6501mg/1100                                  

QY-06280 ZNF300 (zinc finger protein ZNF300)- middle鋅指蛋白300(抗原)                   0.5ml/6501mg/1100                                   

QY-0625R          ZNF268 (zinc finger protein ZNF268)- N-terminal (1a)鋅指脂蛋白-1a(抗原)                    0.5mg/6501mg/1100                                  

QY-06260 ZNF268 (zinc finger protein ZNF268)- middle (1b)鋅指脂蛋白-1b(抗原）               0.5mg/6501mg/1100                                  

QY-06270 ZNF268 (zinc finger protein ZNF268)- middle (1c)人鋅指脂蛋白-1c(抗原)              0.5mg/6501mg/1100                                  【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】

QY-04800 IFN-γ(Interferon γ)Ag-mouse、rat干擾素-γ多肽抗原（大、小鼠）            0.5mg/6501mg/1100                                  

QY-04810 IFN-γ(Interferon γ)Ag-Human干擾素-γ多肽抗原（人）                 0.5mg/6501mg/1100                                  

QY-07840 IFN-β (Interferon β)Anti-mouse,rat干擾素-β抗原                    0.5mg/6501mg/1100                                  

QY-07870 IFN-β (Interferon β)Anti-human干擾素-β抗原                 0.5mg/6501mg/1100                                  

QY-06690 PSCA (Prostate Stem Cell Antigen) 前列腺干細胞抗原                    0.5mg/6501mg/1100                                  

QY-06450 sIgA大鼠分泌型IgA(抗原)               0.5mg/6501mg/1100                                  

QY-09040 Melamine/KLH三聚氰胺與血藍蛋白偶聯(lián)物                   5mg/200010mg/3000                                 

QY-09041 Melamine/RCA三聚氰胺與牛血清白蛋白偶聯(lián)物                  2mg/7805mg/150010mg/2500                                   

QY-09042 Rabbit IgG Sepharose 4B兔IgG親和層析柱                    2ml柱床體積/15005ml柱床體積/2500                                    

133187     NeXtalStock Citric acid pH 5.0 (200)                  CNY                             

51126       QIAamp DNA Blood Mini QIAcube Kit (240)             CNY                             

133096     NeXtalStock Potassium phosphate (200)          CNY                             

133095     NeXtalStock Potassium chloride (200)               CNY                             

959034     EZ1 RNA Tissue Mini Kit (48)   48 Reagent Cartridges (RNA Tissue) 100 Disposable Tip Holders, 100 Disposable Filter-Tips, 50 Sample Tubes (2 ml), 50 Elution Tubes (1.5 ml), Buffer RLT, RNase-Free DNase I       CNY                             

957336     MagAttract Direct mRNA M48 Kit (192)                   CNY                             

9018448  O-ring, purge port, QX              CNY                             

133172     NeXtalStock CHES pH 8.5 (200)                 CNY                             

133171     NeXtalStock CAPSO pH 9.8 (200)               CNY                             

9018408  Regulator, N2, rear, QX            CNY                             

291045     mericon Screen nos Kit (96)              CNY                             

291025     mericon GMO Screen 35S-pat  (96)                 CNY                             

291013     mericon Screen 35S Kit (24)              CNY                             

9001866  Rotor-Gene Q 5plex (PC) PrioPLUS  "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9012326  Set Peristaltic Pump Tubing Plato 3000            CNY                              【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】

9012322  Tubing set, peristaltic pump, 1x15 cm              CNY                             

1070195  VacValve Tool (1)               CNY                             

1067933  Buffer RWT (concentrate, 80 ml)              CNY                             

133080     NeXtalStock PEG 20,000 (200)                   CNY                             

38071       AIL Cartridge (1ml)           CNY                             

38015       #WGA Cartridge (5ml)               CNY                             

204754     QuantiFast Multiplex PCR +R Kit (400)              CNY                             

204643     QuantiTect Multiplex RT-PCR Kit (200)             CNY                             

204574     Rotor-Gene Probe RT-PCR Kit (400)        The Rotor-Gene Probe RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time one-step RT-PCR using sequence-specific probes.         CNY                            

204363     QuantiTect Probe PCR + UNG Kit (200)   The supplied, ready-to-use ｍaster mix contains HotStarTaq DNA Polymerase, ROX passive reference dye, ａnd a dNTP mix in an optimized buffer. The dNTP mix includes dUTP, allowing optional treatment with UNG. The UNG enzyme is provided separately. For convenience, the ｍaster mix can be stored at 2–8°C.     CNY                             

9015350  Board, terminal                 CNY                             

9015349  Board, motor controller           CNY                             

9018402  Kit, purge filter, QX           CNY                             

9018389  QIAcube Tip Adapter Ring Replacement Kit            CNY                             

9012283  Power Supply, 6 amp for P 90, (7135)                CNY                             

9012180  Syringe pump inlet tubing                  CNY                              【人酪氨酸激酶2(Tyk-2)ELISA試劑盒廠家】