價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過(guò)程非常重要，不充分的洗滌易造成假陽(yáng)性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線(xiàn)，做復(fù)孔。

5. 如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高，請(qǐng)先稀釋后再測(cè)定，計(jì)算時(shí)請(qǐng)*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測(cè)溶液工作液時(shí)，請(qǐng)以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請(qǐng)避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QY-18872 Anti-TRA16 (Testicular orphan nuclear receptor-4 (TR4)-associated protein)睪丸激素受體相關(guān)蛋白16抗體                                                

QY-18882 Anti-TRA16 (Testicular orphan nuclear receptor-4 (TR4)-associated protein)睪丸激素受體相關(guān)蛋白/孤兒素受體相關(guān)蛋白抗體                                                       

QY-18892 Anti-TRADD （TNF receptor 1 associated via death domain）有死亡區(qū)的腫瘤壞死因子受體1相關(guān)蛋白抗體                                                   

QY-18902 Anti-TRAF1 （TNF receptor-associated factor 1）腫瘤壞死因子受體相關(guān)因子1抗體                                                  

QY-18912 Anti-TRAF2 （TNF receptor-associated factor 2）腫瘤壞死因子受體相關(guān)因子2抗體                                                  

QY-18922 Anti-TRAF3/CD40bp （CD40 binding protein）CD40結(jié)合蛋白/CD40受體相關(guān)因子1抗體                                                   

QY-18932 Anti-TRAF6 （TNF receptor-associated factor 6）腫瘤壞死因子受體相關(guān)因子6抗體                                                  

QY-18942 Anti-TRAIL/Apo2L (tumor necrosis factor-related apoptosis-inducingligand)腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體/凋亡素2配體抗體                                                   【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】

QY-18952 Anti-Transthyretin (TTR/prealbumin)轉(zhuǎn)甲狀腺素蛋白抗體                                                

QY-18962 Anti-TRBF1 (Telomeric repeat binding factor 1)端粒體復(fù)制結(jié)合因子1抗體                                                    

QY-18972 Anti-TRF1（telomere repeat binding factor1）端粒結(jié)合蛋白1抗體                                               

QY-18982 Anti-TrK A (Tyrosine kinase,TrkA)酪氨酸激酶A抗體(神經(jīng)生長(zhǎng)因子受體的一種)                                                     

QY-18992 Anti-TrK A.B.C(Tyrosine kinase,TrkA.B.C)酪氨酸激酶A.B.C抗體                                                

QY-19002 Anti-Trk B(Tyrosine kinase,Pyk2)酪氨酸激酶B抗體(神經(jīng)生長(zhǎng)因子受體的一種)                                                      

QY-19012 Anti-Trk B(Tyrosine kinase,TrkB)酪氨酸激酶B抗體(神經(jīng)生長(zhǎng)因子受體的一種)                                                       

QY-19022 Anti-Trk C(Tyrosine kinase,TrkC)酪氨酸激酶C抗體(神經(jīng)生長(zhǎng)因子受體的一種)                                              

QY-19032 Anti-Trop-2Trop-2抗體                                                 

QY-19042 Anti-TSARG3（Testis ａnd spermatogenesis related gene 3）生精細(xì)胞凋亡相關(guān)基因3抗體                                                  

QY-19052 Anti-TSARG4（Testis ａnd spermatogenesis related gene 4）生精細(xì)胞凋亡相關(guān)基因4抗體                                                  

QY-19062 Anti-TSGF（Tumor Specific Growth Fanctor）惡性腫瘤特異性生長(zhǎng)因子抗體                                                 

QY-19072 Anti-TSH(Mouse Anti-Human Thyroid stimulating hormone Monoclonal Antibody)鼠抗人促甲狀腺素單克隆抗體                                                

QY-19082 Anti-TSHR (Thyroid stimulating hormone receptor)促甲狀腺素受體抗體                                                  

QY-19092 Anti-TSHR (Thyroid stimulating hormone receptor)（CT）促甲狀腺素受體抗體（C端）                                              

QY-19102 Anti-TSHR (Thyroid stimulating hormone receptor)(NT)促甲狀腺素受體抗體（N端）                                                     【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】

QY-19112 Anti-TSLC1 (tumor suppressor in lung cancer-1)肺癌腫瘤阻抑基因1抗體                                                

QY-19122 Anti-TSLP(Thymic stromal lymphopoietin isoform 1)淋巴細(xì)胞生成素-1抗體                                                     

QY-19132 Anti-TTF-1 (Thyroid Transcription Factor-1)甲狀腺核轉(zhuǎn)錄因子-1/甲狀腺特異增強(qiáng)子結(jié)合蛋白抗體                                                        

QY-19142 Anti-TTF-2(thyroid transcription factor 2;forkhead box E1)甲狀腺轉(zhuǎn)錄因子-2抗體                                                  

QY-19152 Anti-Tubulin-α微管蛋白抗體                                                       

QY-19162 Anti-Tubulin-β微管蛋白（免疫組化用抗體）                                                  

QY-19172 Anti-Tubulin-β微管蛋白抗體                                                       

QY-19182 Anti-Tubulin-γ微管蛋白-γ抗體                                                 

QY-19192 Anti-TY3H/TH (Tyrosine Hydroxylase )酪氨酸羥化酶抗體                                                   

970530     NRAS Pyro Kit (24)            CNY                             

970022     PyroMark Q24 CpG MLH1 (4 x 24)   For quantification of methylation level in region -209 to -188 from the transcription start site. CNY                             

969261     Ni-NTA Superflow 96 BioRobot Kit (4)               CNY                             

133199     NeXtalStock HEPES pH 8.0 (200)               CNY                             

292143     mericon Horse Kit (24)              CNY                             

206047     Type-it Fast SNP Probe PCR Kit (4000)     "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"   CNY                             

292025     mericon Cattle (96)           CNY                             

291113     mericon RR Soy Kit (24)            CNY                             

9001908  QIAgility HEPA / UV (w/o PC) System       QIAgility HEPA / UV (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

133111     NeXtalStock Sodium fluoride (200)           CNY                             

9018540  Kit, installation, Qsym               CNY                             

9240672  Kerk Nut, Common, AP             CNY                             

9240658  Pump, Vacuum, Jun-Air, AP               CNY                             

9240642  Cable Assy, Proteinase K Pump, AP196/98                  CNY                             

9240638  Opto Sensor, Reagent Pump, AP               CNY                              【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】

9240617  Fuse, 2.0A, FNM, AP96             CNY                             

9240589  MAC Valve, 4-Way, Cent. Locator, AP96           CNY                             

9240560  Rotor/Buckets, Eppendorf, AP196/98                CNY                             

9240554  Bucket Grease, Eppendorf, AP196/98                CNY                             

9240523  Cable, Motor, Axis #0, Dispense, AP98             CNY                             

9240507  Software & Std. Protocols, AP196            CNY                             

9240377  QIAcube, Installation       The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.  CNY                             

9239571  BR Universal, Basic Agreement       The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9239570  BR Universal, Premium Agreement         The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).    CNY                             

9020032  FOOTSET, RGQ                   CNY                             

9019986  PCB, small breakout, 12 core, C12            CNY                             

9019479  UPPER BUFFER LID GS              CNY                             

9238864  BR M48, Premium Agreement         The Premium Cover Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238852  LiquiChip, Basic Agreement     The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9241224  QIAxcel, Inspection Service      "Repair service delivery not applicable

Service response time (repair) not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: Yes, included" CNY                             

9241197  Silicone sealant, RTV 108, QX           CNY                              【人T細(xì)胞特異性鳥(niǎo)苷三磷酸酶(Tgtp)ELISA試劑盒廠家】