檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書有異,以英文說(shuō)明書為準(zhǔn).

【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

QY-18542 Anti-Tenascin/TN細(xì)胞粘合素抗體                                                       

QY-18552 Anti-TFF3 (trefoil factor3)三葉肽因子3抗體                                                       

QY-18562 Anti-TFPI-2 (tissue factor pathway in hibitor-2)組織因子途徑抑制劑-2抗體                                                     

QY-18572 Anti-TGF-α (Ttansforming Growth Factor –α)轉(zhuǎn)移生長(zhǎng)因子–α抗體                                               

QY-18582 Anti-TGF-β1（Ttansforming Growth Factor –β1）轉(zhuǎn)移生長(zhǎng)因子–β1抗體                                                     

QY-18592 Anti-TGF-β2 （Ttansforming Growth Factor –β2）轉(zhuǎn)移生長(zhǎng)因子–β2抗體                                                   

QY-18602 Anti-TGF-β3（Ttansforming Growth Factor –β3）轉(zhuǎn)移生長(zhǎng)因子–β3抗體                                                     

QY-18612 Anti-TGF-βR 2（Transforming growth factor β receptor 2 ）轉(zhuǎn)移生長(zhǎng)因子–β受體2抗體                                                   

QY-18622 Anti-TGF-βR1/ALK (TGF-beta receptor type I)轉(zhuǎn)移生長(zhǎng)因子–β受體1抗體                                                  【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】

QY-18632 Anti-Thioster-containing protein 1（Anti-Anopheles stephensi protein 1）硫酯包含蛋白-1抗體                                                    

QY-18642 anti-Thioredoxin 1(ERdj5)硫氧還蛋白抗體                                                  

QY-18652 Anti-Thy-1/CD90CD90抗體                                                   

QY-18662 Anti-Thymosin α-1胸腺肽 α-1抗體                                               

QY-18672 Anti-Thyroxine(T4)/Tg4-Ab (Thyroglobulin 4 Antibody)甲狀腺素T4抗體                                                    

QY-18682 anti-Tie2（Porcine tyrosine kinase with immunoglobulin-like ａnd EGF-like domains 2）上皮生長(zhǎng)因子樣域酪氨酸激酶2                                                    

QY-18692 Anti-TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )兔抗人TIGAR蛋白抗體                                               

QY-18702 Anti-TIMP-1(Tissue Inhibitor of Metalloproteinase-1)金屬蛋白酶組織抑制因子-1抗體                                                  

QY-18712 Anti-TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金屬蛋白酶組織抑制因子-2抗體                                                  

QY-18722 Anti-TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金屬蛋白酶組織抑制因子-3抗體                                                  

QY-18732 Anti-TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金屬蛋白酶組織抑制因子-4抗體                                                  

QY-18742 Anti-TLR2 (Toll-like receptor 2)Toll樣受體2抗體                                               

QY-18752 Anti-TLR4 (Toll-like receptor 4)Toll樣受體4抗體                                               

QY-18762 Anti-TLR5/CD285 (Toll-like receptor 5)Toll樣受體5抗體                                                     

QY-18772 Anti-TM（Thrombomodulin)血栓調(diào)節(jié)蛋白抗體                                                

QY-18782 Anti-TmR2（GDNFRβ；TRN-R；GFR-alpha-2；NTNR-2）膠質(zhì)細(xì)胞系源性神經(jīng)營(yíng)養(yǎng)因子受體β抗體                                                【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】

QY-18792 anti-Tn-C(tenascin-C)腱糖蛋白-C抗體                                                 

QY-18802 Anti-TNF-α (Mouse Anti-Human Tumor Necrosis Factor-α Monoclonal Antibody)鼠抗人腫瘤壞死因子-α單克隆抗體                                                       

QY-18812 Anti-TNF-α(Tumor Necrosis Factor-α)腫瘤壞死因子-α抗體                                                   

QY-18822 Anti-TNF-β(Tumor Necrosis Factor-β)腫瘤壞死因子-β抗體                                                   

QY-18832 Anti-TPⅠ （topoisomeraseⅠ）拓普西異構(gòu)酶Ⅰ抗體                                                     

QY-18842 Anti-DNA TPⅡα (DNA topoisomeraseⅡα)DNA拓普西異構(gòu)酶Ⅱ抗體                                                  

QY-18852 Anti-TPH (Trptophan Hydroxylase)色氨酸羥化酶抗體                                                

QY-18862 Anti-TPO (Thyroid Peroxidase)抗甲狀腺過(guò)氧化物酶抗體                                                   

972442     PyroMark Q96 SNP HFE (96)    For detection of variants in HFE gene      CNY                             

133221     NeXtalStock Sodium ac. H2O pH 5.6 (200)                CNY                             

133220     NeXtalStock Sodium ac. H2O pH 5.0(200)                 CNY                             

301704     HiPerFect Transfection Reagent (0,5 ml)                   CNY                             

301427     Effectene Transfection Reagent (4x1ml) 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates      CNY                             

51326       QIAamp DNA Mini QIAcube Kit (240)                CNY                             

9012672  BGR Peripumpe PP-4 Frontbl.gerade (7135)            CNY                             

9002011  Rotor-Gene Q MDx 2Plex HRM no PC               CNY                             

292063     mericon Sheep Kit (24)              CNY                             

9001912  PyroMark Q24 MDx, PrioPLUS         "PyroMark Q24 MDx Instrument

Installation of PyroMark Q24  & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"         CNY                             

9001910  QIAgility HEPA/UV (w/o PC) PrioPLUS      "QIAgility HEPA/UV (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

133116     NeXtalStock Sodium thiocyanate (200)             CNY                             

967142     RNeasy 96 BioRobot 9604 Kit (12)  For 12 x 96 total ａnd cytoplasmic RNA preps on the BioRobot 9604: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents ａnd Buffers         CNY                             

962141     QIAprep 96 Turbo BioRobot Kit (4)  For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads         CNY                              【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】

960241     QIAGEN Plasmid Plus 96 BioRobot Kit (4)                  CNY                             

51304       QIAamp DNA Mini Kit (50)       For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                             

978756     PyroMark CpG Assay 96 well (200)           CNY                             

9012712  Z-motor (Plato 7) left                 CNY                             

9012696  Set of X-flat band cable (Plato 7)               CNY                             

133239     NeXtalStock Sodium citrate pH 7.0 (200)                  CNY                             

133238     NeXtalStock Sodium citrate pH 5.8 (200)                  CNY                             

9002127  QIAsymphony RGQ, Priority    QIAsymphony RGQ Priority includes the QIAsymphony RGQ instruments, installations ａnd extended warranties. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                            

59203       EpiTect Whole Bisulfitome Kit (25)           CNY                             

59164       EpiTect Plus LyseAll Bisulfite Kit (48)                  CNY                             

929526     QX Alignment Marker 50 bp/1 kb (1.5 ml)                 CNY                             

133218     NeXtalStock MOPS pH 8.0 (200)                CNY                             

301605     RNAiFect Transfection Reagent (1ml)      RNAiFect Reagent ａnd buffer, for up to 170 transfections in 24-well plates; up to 500 transfections in 96-well plates  CNY                             

301525     TransMessenger Tranfect. Reagent(0.5ml)     For 60 transfections in 6-well plates or 80 transfections in 12-well plates         CNY                             

52304       QIAamp RNA Blood Mini Kit (50)     For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers    CNY                             

51306       QIAamp DNA Mini Kit (250)     For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                              【人吡哆醛/吡哆醇維生素B6磷酸酶(PDXP)ELISA試劑盒現(xiàn)貨】

9002012  Rotor-Gene Q MDx 2plex HRM Platform                  CNY