1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

ELISA應用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內生化領域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結果判斷較客觀等因素,已廣泛應用在免疫學檢驗的各領域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】齊一生物科技歡迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】齊一生物供應的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經過溫育和洗滌,去除未結合的酶結合物,然后加入底物A、B,和酶結合物同時作用.產生顏色.顏色的深淺和樣品中的濃度呈比例關系.

免疫測定技術的基礎在于抗原抗體之間的特異結合反應,所以任何優(yōu)質的診斷試劑離不開優(yōu)質的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術得到的單克隆抗體,而抗原或抗體的標記物如酶標結合物則通過各種化學合成方法制備.近年來,隨著分子生物學的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應性.酶表抗體為與HBsAg有不同結合位點的鼠復合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應用ParlEhrich學說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應用定性夾心免疫檢測技術,用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結合物,經第二次孵育后,酶結合物就會與次孵育結合上的HEVIgM抗體相結合,洗板除去未結合的酶結合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應,只有那些含有HEVIgM抗體和酶結合物所形成的復合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應,并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性. 【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質，高靈敏度.高質量的酶聯(lián)免疫試劑盒，齊一生物長期經營ELISA試劑盒（免費代測）及抗體、分子學生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-17882 Anti-Sema3F (semaphorin 3F)臂板蛋白3F抗體                                                  

QY-17892 Anti-Sema4C (semaphorin 4C)臂板蛋白4C抗體                                                 

QY-17902 anti-SFRP1（Secreted frizzled related protein 1）分泌型卷曲相關蛋白1抗體                                                       

QY-17912 Anti-SGC (soluble glycoprotein C)可溶性糖蛋白C抗體                                                       

QY-17922 Anti-SGLT1 (sodium-glucose cotransporter1)鈉-葡萄糖共轉運載體1抗體                                               

QY-17932 Anti-Shank1(SH3 ａnd multiple anleyrin repead domins protein 1)shank-1抗體                                                   

QY-17942 Anti-SHBG (Sex Hormone Binding Globulin)性激素結合球蛋白抗體                                                  

QY-17952 Shiga-like toxin IIe variant subunit ACAS:Escherichia coli抗豬水腫病大腸桿菌志賀樣毒素Ⅱ型突變體（O139菌株）抗體                                                       

QY-17962 Anti-SHP-1 (Src homology phosphatase-1)造血細胞磷酸酶抗體                                                

QY-17972 Anti-SHP2/PTPN11（Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2）蛋白酪氨酸磷酸酶2抗體                                                

QY-17982 Anti-SIP1 (survival of motor neuron protein interacting protein 1)運動神經元存活蛋白結合蛋白1抗體                                                【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】

QY-17992 Anti-Skp2 (S-phase kinase-associated protein 2,SKP2)細胞S相激酶相關蛋白抗體                                                   

QY-18002 Anti-Slc22A17 (solute carrier famili 22A17)可溶性載質轉運蛋白22A17抗體                                                   

QY-18012 Anti-SLC24A5 (solute carrier family 24, member 5)可溶性載質轉運蛋白SLC24A5抗體                                                   

QY-18022 Anti-SLC6A19 (Solute carrier family 6, member 19)鉀依賴鈉鈣交換蛋白6                                               

QY-18032 anti-Smac/DIABLO（the second mitochondrial activator of caspase）線粒體促凋亡蛋白抗體                                              

QY-18042 SLC34A2/NaPi-2b(Solute carrier family 34 member 2)磷酸鈉協(xié)同轉運蛋白抗體                                                       

QY-18052 Anti-Smad2/3(Mothers against decapentaplegic homolog 2)Smad 2/3抗體                                                 

QY-18062 Anti-Smad4(Mothers against decapentaplegic homolog 4)Smad4抗體                                                       

QY-18072 Anti-Smad7(Mothers against decapentaplegic homolog 7)Smad 7抗體                                                      

QY-18082 Anti-SM-MHC（Cardiac myosin heavy chain）心肌肌凝蛋白抗體                                                     

QY-18092 Anti-Snake poison Protein抗蛇毒蛋白抗體（蝮蛇）                                                

QY-18102 Anti-Snake poison Protein抗蛇毒蛋白抗體（中華眼鏡蛇）                                                     

QY-18112 Anti-SNAP-25 (synaptosomal-associated protein 25)突觸相關膜蛋白25抗體                                                   

QY-18122 Anti-Socs 1 (suppressor of cytokine signaling 1)細胞因子信號傳導抑制蛋白1抗體                                                 

QY-18132 Anti-Socs 3 (suppressor of cytokine signaling 3)細胞因子信號傳導抑制蛋白3抗體                                                 

QY-18142 Anti-SOD1 (superoxide-dimutase-1)超氧化物歧化酶1抗體                                                        【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】

QY-18152 Anti-SOD2 (superoxide-dimutase-2)超氧化物歧化酶2抗體                                                       

QY-18162 Anti-Solute carrier family 1溶質攜帶物家族-1抗體                                                   

QY-18172 Anti-Somatostatin/GRIH生長抑素抗體                                                       

QY-18182 Anti-Sox2胚胎干細胞關鍵蛋白抗體                                                  

QY-18192 Anti-SP (SuRCtance P）P物質抗體                                                       

QY-18202 Anti-SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A抗體                                               

9013217  ZLV Screws, plastic, M5 (20)             CNY                             

9013205  BGR Labhand li+re Digene                CNY                             

9013195  Tubing,vac.trap outlet,autov.BR8000v1.00              CNY                             

132572     NeXtal Stock Jeffamine M-600 (50) 100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 50mL (5 x 10 ml tube)         CNY                             

132558     NeXtal Stock Lithium sulfate (50)     1M Lithium sulfate monohydrate - 50mL (4 x 12,5 ml tube)   CNY                             

800333     PCR Design                CNY                             

800301     Heterozygote detection, automated      "PCR amplification of genomic DNA

QIAGEN DNA template preparation

Sequencing ａnd sequence alignment

Automatic detection of mutations and

heterozygotic positions

Mutation report

Accuracy >99%"       CNY                             

800251     Single Read HT, w/o prep                  CNY                             

800151     Basic Read Plates              CNY                             

4514263  artus BK Virus RG PCR Kit (24) CE             CNY                             

4513233  artus HI Virus-1 RG RT-PCR Kit (24) RUO                   CNY                             

130710     NeXtal Tubes pHClear II Suite           CNY                              【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】

130707     NeXtal Tubes Anions Suite                 CNY                             

120007     Collection Microtubes ａnd Caps       Nonsterile polypropylene tubes (1.2 ml), 2304 in packs of 96, ａnd nonsterile polypropylene caps for Collection Microtubes          CNY                             

79216       Buffer RLT         220 ml RNeasy Lysis Buffer       CNY                             

76154       RNAlater TissueProtect Tubes (50x1,5ml)       For stabilization of RNA in 50 x 150 mg tissue samples: 50 screw-top tubes containing 1.5 ml RNAlater RNA Stabilization Reagent each CNY                             

74524       RNeasy Protect Bacteria Mini Kit (50)     RNeasy Mini Kit (50) ａnd RNAprotect Bacteria Reagent (2 x 100 ml)     CNY                             

69980       TissueLyser LT Adapter, 12-Tube               CNY                             

19530       Vacuum Regulator   For use with QIAvac manifolds         CNY                             

19081       Buffer AW1       242 ml Wash Buffer (1) Concentrate for 1000 spin, 250 midi, or 100 maxi preps   CNY                             

19064       Buffer N3 500 ml Neutralization Buffer   CNY                             

19055       Buffer QC 1000 ml Wash Buffer        CNY                             

13362       Blood & Cell Culture DNA Maxi Kit  10 QIAGEN Genomic-tip 500/G, QIAGEN Protease, Buffers   CNY                             

12843       CompactPrep Plasmid Midi Kit (25) "For ultrafast purification of up to 200 µg or 750 µg Molecular Biology Grade plasmid or cosmid DNA for standard applications

CompactPrep Plasmid Kits enable fast, large-scale plasmid purification in a miniprep format. The use of a vacuum manifold allows 24 samples to be purified in parallel on a single workbench"       CNY                             

4503063  artus CMV LC PCR Kit (24) CE            CNY                             

4502303  artus VZV QS-RGQ Kit (24) RUO                CNY                             

4502163  artus VZV TM PCR Kit (24) CE           CNY                             

990558     Reagent Trough Lid 270 mL (15)               CNY                              【人血清/糖皮質激素調節(jié)激酶2(SGK2)ELISA試劑盒現(xiàn)貨】

337016     qBiomarker Mutation Assay Control DNA                 CNY                             

4502003  artus VZV LC PCR Kit (24) RUO                  CNY                             

949016     Autopure 100% Isopropanol (3800 ml)             CNY