檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果. 【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

計算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

QT-00417 銅綠假單胞桿菌（綠膿桿菌）     ATCC 27853      2支 美國 MBL        1400                  

QT-00418 銅綠假單胞桿菌（綠膿桿菌）     ATCC 27853      5支 美國 MBL        2620                  

QT-00419 銅綠假單胞桿菌（綠膿桿菌）     ATCC 9027        支     上海         460           

QT-00420 銅綠假單胞桿菌（綠膿桿菌）     ATCC 9027        2支 美國 MBL        1800                  

QT-00421 銅綠假單胞桿菌（綠膿桿菌）     ATCC 9027        5支 美國 MBL        3000                  

QT-00422 金黃色葡萄球菌     ATCC 27217      支     上海         800           

QT-00423 金黃色葡萄球菌     ATCC 6538        支     上海         460           

QT-00424 金黃色葡萄球菌     ATCC 6538        2支 美國 MBL        1700                  

QT-00425 金黃色葡萄球菌     ATCC 6538        5支 美國 MBL        3000                  

QT-00426 白色假絲酵母/白色念球菌   ATCC 10231      支     上海         400           

QT-00427 白色假絲酵母/白色念球菌   ATCC 10231      2支 美國 MBL        1810                  

QT-00428 白色假絲酵母/白色念球菌   ATCC 10231      5支 美國 MBL        3000                   【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】

QT-00429 大腸埃希氏菌         ATCC 8739        支     上海         460           

QT-00430 大腸埃希氏菌         ATCC 8739        2支 美國 MBL        1550                  

QT-00431 表皮葡萄球菌         ATCC 12228      支     上海         400           

QT-00432 表皮葡萄球菌         ATCC 12228      2支 美國 MBL        1550                  

QT-00433 表皮葡萄球菌         ATCC 12228      5支 美國 MBL        2620                  

QT-00434 表皮葡萄球菌                   支     上海         460           

QT-00435 肺炎克雷伯菌                   支     上海         460           

QT-00436 陰溝腸桿菌              支     上海         500           

QT-00437 陰溝腸桿菌     ATCC 13047      支     上海         820           

QT-00438 洋蔥假單胞菌         ATCC 25416      支     上海         820           

QT-00439 熒光假單胞菌                   支     上海         460           

QT-00440 熒光假單胞菌         ATCC 13525      支     上海         820           

QT-00441 惡臭假單胞菌                   支     上海         460           

QT-00442 惡臭假單胞菌         ATCC 17485      支     上海         820           

QT-00443 黃桿菌屬         ATCC 21047      支     上海         820           

QT-00444 近平滑假絲酵母              支     上海         460            【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】

QT-00445 黑曲霉     ATCC 16404      支     上海         460           

QT-00446 黑曲霉     ATCC 16404      2支 美國 MBL        1950                  

QT-00447 黑曲霉     ATCC 16404      5支 美國 MBL        3000                  

QT-00448 桔青霉              支     上海         460           

QT-00449 黃曲霉              支     上海         460           

9238662  Lid with window                CNY                             

9238659  Cooling compressor T2168GK, 60Hz                  CNY                             

9238645  Brake resistor 112 Ohm / 100 W               CNY                             

9242061  Pipetting container, IQ/OQ, QS                 CNY                             

9241827  Pyro Q96 ID, Premium Agreement "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241166  Board, A/D, QX                  CNY                             

9240955  Sensor, z, handler, Qsym           CNY                             

9240835  QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.  CNY                             

9240766  32 OUT Module, D2-32TD1, AP96            CNY                             

6000-5018        MST STM Rack & Lid (Blue - 2 pos. cal.)            CNY                             

9241777  QIAxtractor, Inspection Service       "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY                              【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】

9241375  Magnet, door lock, top, EZ1a           CNY                             

9241334  Keypad foil, EZ1                 CNY                             

9240750  Belt, Elevator, AP              CNY                             

9243648  Acceler. Valid Supp, Other Extract Sys     The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).          CNY                             

9243553  #BR M96, PM Service      "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242864  QIArent&go -Rent out fee QSSP/AS Cabinet             CNY                             

5000-1321        Feet Set/4, SKR1                CNY                             

5000-1288        DML 2000 - Fuse 160 mA PKG 10              CNY                             

5000-1287        DML 2000 - Fuse 315 mA PKG 10              CNY                             

5000-1505        Switch ON-OFF 120V, HTR1               CNY                             

6000-00181      Plate Washer - Replacement Tubing Kit           CNY                             

6000-00178      Plate Washer - Maintenance Kit               CNY                             

5100-00047      Wash Buffer Concentrate (2 Bottles)       "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"      CNY                             

9240740  Tubing, Tygon, 1/4" ID                CNY                             

9240735  Tubing, Black, Capper, AP196/98               CNY                             

9240724  Tube Assy, Draw, AP96/196                CNY                              【人1,25二羥基維生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA試劑盒廠家】

5000-1412        Poteneiometer 500K, VTX2               CNY                             

9240506  Monitor, 17", Flat Panel, AP             CNY                             

9239851  Cover, LC200             CNY                             

9239565  xMAP Alignment Microspheres                 CNY