1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫(xiě)作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QT-00384 大麗花輪枝孢ACCC30308      凍干粉                                         

QT-00385 白淺灰鏈霉菌ACCC40001      凍干粉                                         

QT-00386 抗生鏈霉菌ACCC40070 凍干粉                                         

QT-00387 天藍(lán)色鏈霉菌ACCC40009      凍干粉                                         

QT-00388 淡天藍(lán)色鏈霉菌ACCC40010 凍干粉                                         

QT-00389 黃直絲鏈霉菌ACCC40046      凍干粉                                         

QT-00390 弗氏鏈霉菌ACCC40012 凍干粉                                         

QT-00391 刺孢吸水鏈霉菌ACCC40018 凍干粉                                         

QT-00392 紫色直絲鏈霉菌ACCC40032 凍干粉                                         

QT-00393 刺孢吸水鏈霉菌昆明變種ACCC40020 凍干粉                                         

QT-00394 吸水鏈霉菌紫色變種ACCC40034 凍干粉                                         

QT-00395 淡紫灰鏈霉菌ACCC40024      凍干粉                                         

QT-00396 孔雀石綠鏈霉菌ACCC40026 凍干粉                                          【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】

QT-00397 玫瑰褐鏈霉菌ACCC40123      凍干粉                                         

QT-00398 珊瑚色諾卡氏菌ACCC40100 凍干粉                                         

QT-00399 雞腿蘑（毛頭鬼傘）ACCC50400  凍干粉                                         

QT-00400 雞油菌ACCC50878 凍干粉                                         

QT-00401 寬圓羊肚菌ACCC50759 凍干粉                                         

QT-00402 長(zhǎng)根菇（長(zhǎng)根奧德磨）ACCC50053       凍干粉                                         

QT-00403 異型酒香酵母ACCC20234      凍干粉                                         

QT-00404 嗜酸乳桿菌ACCC11073 凍干粉                                         

QT-00405 巨大芽孢桿菌ACCC11011      凍干粉                                         

QT-00406 褐球固氮菌ACCC10096 凍干粉                                         

QT-00407 枯草芽孢桿菌ACCC10157      凍干粉                                         

QT-00408 紅平紅球菌ACCC10543 凍干粉                                         

QT-00409 谷氨酸棒桿菌ACCC16522      凍干粉                                         

QT-00410 蠟蚧輪枝孢菌ACCC32146      凍干粉                                         

QT-00411 涇陽(yáng)鏈霉菌ACCC40126 凍干粉                                         

QT-00412 諾爾斯氏鏈霉菌ACCC40029 凍干粉                                          【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】

QT-00413 吸水鏈霉菌應(yīng)城變種ACCC40053 凍干粉                                         

QT-00414 刺孢吸水鏈霉菌北京變種ACCC40074 凍干粉                                         

QT-00415 淺灰鏈霉菌杭州變種ACCC40075 凍干粉                                         

QT-00416 銅綠假單胞桿菌（綠膿桿菌）     ATCC 27853      支     上海         400           

9013308  Base, vacuum manifold, v1.04                   CNY                             

9013298  Top plate, vacuum manifold96 plate , 4           CNY                             

9013236  Tubing set, vacuum, 200, 0               CNY                             

9013224  Gripper set, V-handler, BR3000                 CNY                             

9013197  Tubing, L1 outlet, auto vac,BR8000 v1.00                CNY                             

9011702  Cable, PCON98 to AC-box (level sensor),          CNY                             

9011681  Dispenser drive, BR8000          CNY                             

132635     NeXtal Stock Zinc chloride (50)        0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)  CNY                             

132578     NeXtal Stock PEG 2,000 DME (50)   50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)         CNY                             

800341     Cosmid End-Sequencing, w/ prep              CNY                              【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】

800308     siRNA Hairpin Run w/ prep                CNY                             

800121     Verification Sequencing, w/ prep     "Double-stranded sequencing of a given sequence

QIAGEN® DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99.99%" CNY                             

132528     NeXtal Stock CAPSO untit. (50)         0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)         CNY                             

132510     NeXtal Stock ADA untit. (50)   0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)   CNY                             

764114     PAXgene Bone Marrow RNA Tubes (50)           CNY                             

679913     ipsogen RT Kit (33)            CNY                             

203643     HotStarTaq Plus Master Mix Kit (250)     "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."  CNY                             

201225     Taq PCR Core Kit (1000)   "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

201203     Taq DNA Polymerase (250)      "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180922     GeneRead Adapter L Set 1plex (12)                   CNY                             

180912     GeneRead Adapter I Set 1plex (12)          CNY                             

159214     QIAcard FTA Indicator Four Spots (25)              CNY                             

159201     QIAcard FTA One Spot (100)              CNY                             

9235523  Needle cleaning assembly P/N 4567-01            CNY                             

9235300  Plunger tip, syringe 2,5ml                  CNY                             

9235117  Fuse F4A, v1.00                  CNY                              【人可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒現(xiàn)貨】

9022818  EZ1 Advanced XL Flip Cap Tube Rack                 CNY                             

9234889  Vacuum manifold, 96 seal                  CNY                             

9234862  System liquid container (2l Nalgene)                 CNY                             

9239555  PCBA, Laser Driver, Unlimited          CNY                             

9238664  Hinge                 CNY                             

9020593  Lid, DML3000           CNY                             

9022383  Original Transport Box, QXA             CNY                             

QT-00316 米曲霉CICC2014     凍干粉                                         

QT-00317 弗氏檸檬酸桿菌CICC20211  凍干粉                                         

QT-00318 謝氏丙酸桿菌CICC10019       凍干粉                                         

QT-00319 許旺酵母變種CCRC20332      凍干粉                                         

QT-00320 大腸桿菌CCRC11447      凍干粉                                         

QT-00321 DSMZ18235     凍干粉                                         

QT-00322 巨大芽孢桿菌ACCC11107      凍干粉                                         

QT-00323 球型芽孢桿菌ACCC11096      凍干粉                                         

QT-00324 枯草芽孢桿菌ACCC10627      凍干粉                                         

QT-00325 黃色短桿菌ACCC11063 凍干粉                                         

QT-00326 產(chǎn)黃纖維單胞菌ACCC11055 凍干粉                                         

QT-00327 保加利亞乳桿菌ACCC10638 凍干粉                                         

QT-00328 干酷乳桿菌ACCC10639 凍干粉                                         

QT-00329 植物乳桿菌ACCC11095 凍干粉                                          【人骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒現(xiàn)貨】

QT-00330 變形桿菌ACCC11002      凍干粉                                         

QT-00331 惡臭假單胞菌ACCC10085      凍干粉                                         

QT-00332 藤黃八疊球菌ACCC11001      凍干粉                                         

QT-00333 乳酸鏈球菌ACCC10653 凍干粉                                         

QT-00334 乳酸鏈球菌ACCC11092 凍干粉                                         

QT-00335 乳酸鏈球菌ACCC11093 凍干粉                                         

QT-00336 乳酸鏈球菌ACCC11094 凍干粉                                         

QT-00337 蠟狀芽孢桿菌ACCC11077      凍干粉                                         

QT-00338 熒光威克酵母ACCC20058      凍干粉                                         

QT-00339 間型酒香酵母ACCC20233      凍干粉                                         

QT-00340 葡萄芽假絲酵母ACCC20201 凍干粉                                         

QT-00341 近平滑假絲酵母ACCC20221 凍干粉                                         

QT-00342 熱帶假絲酵母ACCC20005      凍干粉                                         

QT-00343 產(chǎn)朊假絲酵母ACCC20060      凍干粉                                         

QT-00344 漢遜德巴利酵母ACCC20010 凍干粉                                         

QT-00345 健強(qiáng)地霉ACCC20017      凍干粉                                          【人骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒現(xiàn)貨】

QT-00346 阿拉伯糖醇漢遜酵母ACCC20205 凍干粉                                         

QT-00347 杰丁漢遜酵母ACCC20019      凍干粉                                         

9011608  BGR Kamm für Stirrer PK80 neu                CNY                             

9013178  Gripper set, BR8000 v 1.00               CNY                             

9013170  Tubing, syringe inlet 2, BR8000                 CNY                             

9011417  Klemmplättchen Varispan P7 (Alodine)             CNY                             

5050-1020EN   #Keyboard English Spare          CNY                             

9240731  Tubing, 3/8"OD , Blue, AP                   CNY                             

676091     ipsogen CBFB-MYH11 A Standards           CNY                             

9011341  Microplate Washer Module Plato 3                   CNY                              【人骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒現(xiàn)貨】

9011203  Vacuum control unit, BR8000           CNY                             

673522     ipsogen JAK2 MutaQuant Kit (12) CE                 CNY                             

337839     qBiomarker SYBR Fluor Mastermix 25 ml                  CNY                             

337632     qBiomarker SYBR Green ROX Mastermix (12)                   CNY                             

9018907  36-Well Rotor            CNY                             

9018783  CAT, Qsym                  CNY                             

9018694  EZ1 Adv. XL DNA Bacteria Card                 CNY                             

9018565  Door lock, mechanical, EZ1a            CNY                             

9013132  Elevating Platform            CNY                             

4509063  artus WNV LC RT-PCR Kit (24) CE              CNY                             

9001905  #QIAgility (w/o PC) System       QIAgility (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                             

800383     Open Reading Frame Search            CNY                             

9019052  Notebook 2 XP          CNY                             

9020200  ZLV PCB, USB, 18Q            CNY                             

9020580  Cable, DB9 M/F (UL) 6ft, DML 3000                  CNY                             

9020588  Pulley, belt, DML 3000              CNY                              【人骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒現(xiàn)貨】

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9013376  Maintenance Kit BR 8000 Research                  CNY                             

9013279  BGR Upgrade-Kit Scanner PK80                CNY                             

9013272  Peristaltic Pump, RCS1              CNY                             

9013190  Motor, rotor, buffer track, BR8000          CNY                             

132772     NeXtal Stock Jeffamine M-600 (200)        100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)       CNY                             

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