1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

以上資料僅供參考了解，不供于論文寫作

【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

    腎上皮細(xì)胞  5 × 105    1ml            

    輸尿管上皮細(xì)胞  5 × 105    1ml            

    腎實(shí)質(zhì)細(xì)胞  5 × 105    1ml            

    腎系膜細(xì)胞  5 × 105    1ml            

    膀胱上皮細(xì)胞    5 × 105    1ml            

    膀胱平滑肌細(xì)胞  5 × 105    1ml            

    腎動(dòng)脈內(nèi)皮細(xì)胞  5 × 105    1ml            

    腎動(dòng)脈平滑肌細(xì)胞    5 × 105    1ml            

    腎成纖維細(xì)胞    5 × 105    1ml             【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】

    膀胱成纖維細(xì)胞  5 × 105    1ml            

    尿道上皮細(xì)胞    5 × 105    1ml            

    胎盤羊膜細(xì)胞    5 × 105    1ml            

    胎盤滋養(yǎng)層細(xì)胞  5 × 105    1ml            

    胎盤絨毛膜細(xì)胞  5 × 105    1ml            

    子宮平滑肌細(xì)胞  5 × 105    1ml            

    子宮成纖維細(xì)胞  5 × 105    1ml            

    卵巢上皮細(xì)胞    5 × 105    1ml            

    卵巢成纖維細(xì)胞  5 × 105    1ml            

    卵巢微血管內(nèi)皮細(xì)胞  5 × 105    1ml            

    子宮內(nèi)膜上皮細(xì)胞    5 × 105    1ml            

    淋巴內(nèi)皮細(xì)胞    5 × 105    1ml            

    淋巴成纖維細(xì)胞  5 × 105    1ml            

    淋巴管內(nèi)皮細(xì)胞  5 × 105    1ml            

    臍帶單核細(xì)胞    5 × 105    1m             

    外周血白細(xì)胞    5 × 105    1m              【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】

    乳腺上皮細(xì)胞    5 × 105    1ml            

    乳腺成纖維細(xì)胞  5 × 105    1ml            

    前列腺上皮細(xì)胞  5 × 105    1ml            

    胰島β細(xì)胞  5 × 105    1ml            

    前列腺平滑肌細(xì)胞    5 × 105    1ml            

    前列腺成纖維細(xì)胞    5 × 105    1ml            

    甲狀腺濾泡上皮細(xì)胞  5 × 105    1ml            

    胰腺星狀細(xì)胞    5 × 105    1ml            

9240751 Belt, TR,Capper U/D & I/O, AP       CNY            

9243546 QIAsymphony SP AS, PM Service   "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9243312 Differential Wash Protocol Pack, QIAcube        CNY            

9242763 QS RGQ, Full Agreement  "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 48 hours/2 business days

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage"   CNY            

9242547 SDS Canbus Cable, LC        CNY            

5000-1289   DML 2000 - Power Cord - 220V Switzerland        CNY            

5000-1286   Fuse 2 AMP PKD 10, DML2     CNY            

5000-1264   Cable Modem INST 9 PIN, DML2        CNY            

5000-1507   Microplate Heater I - Fuse Inlet 3A 250V        CNY            

6000-00180  Plate Washer - Rinse Cap Assembly       CNY            

6000-00179  Plate Washer - Wash Cap Assembly        CNY            

5000-1424   Brush Motor 2 Required, VTX2        CNY            

5000-1421   Block Support Back 099, VTX2        CNY            

5080-1000   Screw Caps      CNY             【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】

PUREGST2    GST-tag Purification ａnd Detection Kit 2        CNY            

5000-1411   Poteneiometer 100L OHM, VTX2        CNY            

6000-5026   hc2 Sample Conversion Tubes 15mL        CNY            

L2127   hc2 High-Risk HPV DNA Test PI (CD-ROM)      CNY            

12943   QIAGEN Plasmid Plus Midi Kit (25)   "25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."  CNY            

9240710 Filter, Pump, Jun-Air, Inlet/Outlet, AP     CNY            

9240709 Filter, Pressure, 5 Micron, AP196/98        CNY            

9242094 QIAcube, Inspection Agreement   "Repair service delivery: not applicable

Service response time (repair): not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, for Periodic Inspection parts

Cost coverage for labor: yes, included for Periodic Maintenance

Cost coverage for travel: yes, included for Periodic Maintenance

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9242095 QIAxcel, Inspection Agreement       CNY            

9241331 #QIAxcel, Full Agreement, no PM The Full Agreement QIAxcel covers all travel, labor ａnd repair part costs related to an instrument repair for 1 year. Service response time is 2 working days.  CNY            

9001772 careHPV test System     CNY            

275703  flocktype Salmonella Ab (5)     CNY            

270045  cattletype BHV1 gB Ab (20)      CNY            

274013  flocktype AIV Ab (5)        CNY            

289993  virotype Tissue Lysis Reagent (250 ml)      CNY            

281807  virotype CSFV RT-PCR Kit (480)      CNY             【人橫紋肌輔肌動(dòng)蛋白α(smActinin-α)ELISA試劑盒現(xiàn)貨】

273403  pigtype Toxoplasma Ab (5)       CNY