【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

       結(jié)腸癌組織源性原代細(xì)胞    5 × 105/1ml                            

       直腸癌組織源性原代細(xì)胞    5 × 105/1ml                            

       肺癌組織源性原代細(xì)胞       5 × 105/1ml                            

       膽道癌組織源性原代細(xì)胞    5 × 105/1ml                            

       腎癌組織源性原代細(xì)胞       5 × 105/1ml                            

       膀胱癌組織源性原代細(xì)胞    5 × 105/1ml                            

       胰腺癌組織源性原代細(xì)胞    5 × 105/1ml                            

       甲狀腺癌組織源性原代細(xì)胞       5 × 105/1ml                            

       前列腺癌組織源性原代細(xì)胞       5 × 105/1ml                            

       乳腺癌組織源性原代細(xì)胞    5 × 105/1ml                            

       卵巢癌組織源性原代細(xì)胞    5 × 105/1ml                             【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】

       子宮癌組織源性原代細(xì)胞    5 × 105/1ml                            

       宮頸癌組織源性原代細(xì)胞    5 × 105/1ml                            

       皮膚癌組織源性原代細(xì)胞    5 × 105/1ml                            

       膠質(zhì)瘤組織源性原代細(xì)胞    5 × 105/1ml                            

       髓母細(xì)胞瘤組織源性原代細(xì)胞    5 × 105/1ml                            

       胃癌組織源性原代成纖維細(xì)胞    5 × 105/1ml                            

       肺微血管內(nèi)皮細(xì)胞       5 × 105       1ml                      

       肺大動(dòng)脈內(nèi)皮細(xì)胞       5 × 105       1ml                      

       肺大動(dòng)脈平滑肌細(xì)胞    5 × 105       1ml                      

       肺大靜脈內(nèi)皮細(xì)胞       5 × 105       1ml                      

       肺大靜脈平滑肌細(xì)胞    5 × 105       1ml                      

       肺動(dòng)脈成纖維細(xì)胞       5 × 105       1ml                      

       肺泡上皮細(xì)胞       5 × 105       1ml                      

       氣管上皮細(xì)胞       5 × 105       1ml                      

       支氣管上皮細(xì)胞    5 × 105       1ml                      

       小氣道上皮細(xì)胞    5 × 105       1ml                       【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】

       肺成纖維細(xì)胞       5 × 105       1ml                      

       支氣管平滑肌細(xì)胞       5 × 105       1ml                      

       小氣道平滑肌細(xì)胞       5 × 105       1ml                      

       氣管平滑肌細(xì)胞    5 × 105       1ml                      

       支氣管成纖維細(xì)胞       5 × 105       1ml                      

       心房肌細(xì)胞    5 × 105       1ml                      

9237329  Upgrade, BR & 3 ext. devices w. sched.      Upgrade BioRobot with three approved integration devices using scheduling software  CNY                           

9237168  Bottle Sheath Supply             CNY                           

9233004  Fan 24V, for BR3000 with VariTerm            CNY                           

9237151  Power Supply        CNY                           

9237123  Upgrade App., QIAquick 96 PCR  On-site application Upgrade for standard QIAGEN QIAquick 96 including kit   CNY                           

9237106  Protocol Dev./Opt., on-site (2-days)     The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed. CNY                           

9237097  Training, BR QIAGEN Advanced  A minimum of 4 people are required to attend. The QIAGEN BioRobot Systems Advanced Training includes three days of  training at our training facility to introduce users to advanced software topics for the  QIAGEN BioRobot Systems.  Designed for users with a basic understanding of the use ａnd programming of QIAGEN BioRobot systems who want to write complex protocols for their instrument, the advanced training starts with a brief review of basic concepts such as teaching slots ａnd racks before exploring the higher-level programming features of the QIAsoft Operating System.  Topics include the structure ａnd use of functions, using variable for programming flexibility, IF/ELSE/END boolean logic commands, ａnd parameterized pipetting using tables.  Users will write, test ａnd run protocols they have developed to demonstrate ａnd reinforce training success.    CNY                           

9236422  Power supply, replacement kit, TWI            CNY                           

9232968  Print YZ96-Adapter-Board Plato-7        CNY                           

9236363  Reservoir XYP              CNY                           

9236353  Tubing Sample Needle to 3-Way Valve         CNY                           

9236329  2-Way Valve (Sheath or vent)        CNY                            【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】

9236160  Potentionmeter, rotary, kit, RapidPlate          CNY                           

9236154  Online Fill Pump RP             CNY                           

9236126  QIAGEN Multiwell-Plate Holder , silver       Holder for accommodating multiwell plates on the Microplate station of the BioRobot MDx worktable      CNY                           

9236120  Runner, Drawer, BR8 RD             CNY                           

9235790  Ramp, Locking, Drawer, BR8 RD        CNY                           

9230745  Z-Lead screw for Plato 7             CNY                           

9235690  Cable X-Main, RCS1            CNY                           

9235572  Shaker Tool, RCS1        CNY                           

9230314  Cable, RS232, syringe pump, 225mm          CNY                           

9230283  Cable, Interface, X-main to Washer             CNY                           

5000-1302      Capacitor 2UF, SKR1            CNY                           

5000-1284      DML 2000 - Power Cord, 220V D Type (GB)            CNY                           

5000-1254      Power Supply PCB, DML2           CNY                           

5000-1250      CPU DML 2000 ASSRY Kit, DML2             CNY                           

6000-3401      RCS Upgrade Package          CNY                           

6000-3116      Plate Washer I - 105 Micron Filters             CNY                            【人脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒現(xiàn)貨】

6000-00183    Plate Washer - Manifold        CNY                           

6000-00177    Plate Washer - Syringe Assembly         CNY                           

5000-1501      Holder Fuse, HTR1        CNY