價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【人蛋白脂質(zhì)蛋白抗體(PLP)ELISA試劑盒廠家】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過(guò)程非常重要，不充分的洗滌易造成假陽(yáng)性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高，請(qǐng)先稀釋后再測(cè)定，計(jì)算時(shí)請(qǐng)*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測(cè)溶液工作液時(shí)，請(qǐng)以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請(qǐng)避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人蛋白脂質(zhì)蛋白抗體(PLP)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人蛋白脂質(zhì)蛋白抗體(PLP)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人蛋白脂質(zhì)蛋白抗體(PLP)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

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QY-L0523 2”-O-沒(méi)食子�；鸾z桃苷53209-27-1       2”-O-Galloylhyperin       53209-27-1       20mg                           【人蛋白脂質(zhì)蛋白抗體(PLP)ELISA試劑盒廠家】

QY-L0524 金絲桃素548-04-9 Hypericin 548-04-9  20mg                          

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QY-L0526 去芹菜糖桔梗皂苷D78763-58-3   Deapio-platycodin D         78763-58-3       20mg                          

QY-L0527 桔梗皂苷元22327-82-8         platycodigenin 22327-82-8     20mg                          

QY-L0528 梔子苷24512-63-8 Geniposide       24512-63-8       20mg                          

QY-L0529 京尼平苷酸27741-01-1 Geniposidic acid       27741-01-1       20mg                          

QY-L0530 京尼平6902-77-8   Genipin    6902-77-8         20mg                          

QY-L0531 6-姜酚23513-14-6          6-Gingerol        23513-14-6     20mg                          

QY-L0532 8-姜酚30462-35-2          8-Gingerol        30462-35-2     20mg                          

QY-L0533 10-姜酚23513-15-7         10-Gingerol      23513-15-7       20mg                          

QY-L0534 6-姜烯酚555-66-8  6-Shogaol          555-66-8  20mg                          

QY-L0535 姜酮122-48-5 Zingerone         122-48-5  20mg                          

QY-L0536 劍麻皂苷元77-60-1        Tigogenin         77-60-1    20mg                          

QY-L0537 吉馬酮6902-91-6   Germacrone    6902-91-6         20mg                          

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QY-L0541 蓮心堿2586-96-1   Liensinine          2586-96-1         20mg                          

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QY-L0543 甲基蓮心堿2292-16-2   Neferine  2292-16-2         20mg                          

QY-L0544 異蓮心堿6817-41-0        Isoliensinine     6817-41-0         20mg                          

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9019653  Base Plate VPT, PyroQ96VW             CNY                             

9019626  Tip ejector, Cx2                  CNY                              【人蛋白脂質(zhì)蛋白抗體(PLP)ELISA試劑盒廠家】

9019603  Pins, locating, Mix ａnd Reagent set, CG1                  CNY                             

9019598  Lead, vac trap sensor, CX1                 CNY                             

9019573  Belt, X-axis assembly, CQx                  CNY                             

9019546  Clamp, cable, 3 wires 2 tabs, Cxx              CNY                             

9019533  PCB, USB, QGY                   CNY                             

9019518  RISER VACUUM 16.25MM                CNY                             

217004     miRNeasy Mini Kit (50)             CNY                             

204243     QuantiTect SYBR Green RT-PCR Kit (200)         For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect SYBR Green RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water        CNY                             

9019439  Heating block assy            CNY                             

9019425  Gasket, lower chamber, RG3            CNY                             

9019416  Box, shipping with foam packing, RG3              CNY                             

9019401  Connector, COM incl. int. cable, RG3                CNY                             

9019360  Switch, mains voltage selector, RG6                 CNY                             

1027417  Flexitube siRNA 5 nmol             CNY                             

9019339  LED, green (530), RG6              CNY                             

9019331  EPROM, IC Motherbrd, state FW, RG6             CNY                              【人蛋白脂質(zhì)蛋白抗體(PLP)ELISA試劑盒廠家】

9019237  Linear guide - X, PyroQ24                  CNY                             

9234673  X-Assembly Turntable Card                CNY                             

9234659  Cassette, HSPS, click＇n＇go                  CNY                             

1026956  Buffer AVE (Elution Buffer) (125/125),KG          CNY                             

1026905  RNeasy 96 Plates (12)                CNY                             

9233674  Probe, ceramic coat., 0.4mm, 158mm             CNY                             

9233630  Mousepad BR 01/10 (WW)                 CNY                             

9019209  Motor - Gripper, PyroQ96MD           CNY                             

9019197  Damper kit - Robot, PyroQ96MD              CNY                             

1020955  Buffer AW2 with Azid (127/500),KG           CNY                             

1062791  HBV PCR REAGENT III               CNY                             

9232903  Spritzenpumpe mMBP 2021C P7xxx                  CNY                             

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