1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】齊一生物科技歡迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標記物如酶標結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性. 【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學(xué)生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

NG0530   旋毛蟲抗原檢測試劑盒                   血清         20T/40T                      

NG0531   旋毛蟲抗體IgG\IgM檢測試劑盒          血清         20T/40T                      

NG0532   肺吸蟲抗體IgG\IgM檢測試劑盒          血清         20T/40T                      

NG0533   肝吸蟲抗體IgG\IgM檢測試劑盒          血清         20T/40T                      

NG0534   瘧原蟲抗體IgG\IgM檢測試劑盒          血清         20T/40T                       【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】

NG0536   真菌分離培養(yǎng)基試劑盒                            10T                    

NG0537   念珠菌顯色分離培養(yǎng)基試劑盒                       18T                    

NG0538   皮膚癬菌鑒別培養(yǎng)基試劑盒                            20T                    

NG0539   霉菌滴蟲培養(yǎng)基試劑盒                   分泌物     20T                    

NG0540   杜克雷嗜血桿菌選擇性培養(yǎng)基試劑盒                            20T                    

NG0541   大腸菌群快速檢測紙片                            60T/100T                   

NG0542   幽門螺桿菌快速檢測試劑     脲酶法     胃粘膜/牙垢   50T/100T                   

NG0543   解脲支原體培養(yǎng)基試劑盒液體                       20T                    

NG0544   支原體固體培養(yǎng)基試劑盒                       20T                    

NG0545   解脲-人型支原體復(fù)合培養(yǎng)基試劑盒                      20T                    

NG0546   淋球菌培養(yǎng)管                   分泌物     20T                    

NG0547   淋球菌TM培養(yǎng)基試劑盒               分泌物     10T                    

NG0548   淋球菌鑒定藥敏試劑              分泌物     20T                    

NG0549   解脲支原體選擇性培養(yǎng)基試劑盒                   分泌物     20T                    

NG0550   解脲支原體藥敏盒                   分泌物     20T                    

NG0551   肺炎支原體選擇性培養(yǎng)基試劑盒                   分泌物     20T                     【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】

NG0552   人型支原體選擇性培養(yǎng)基試劑盒                   分泌物     20T                    

NG0553   人型支原體藥敏盒                   分泌物     20T                    

NG0554   生殖支原體選擇性培養(yǎng)基試劑盒                   分泌物     20T                    

NG0555   霉菌培養(yǎng)基試劑盒                   分泌物     20T                    

NG0556   自然沉降法液基細胞處理試劑盒         100人份/件    88元/人份                                  

NG0557   宮頸脫落細胞處理試劑盒     100人份/件    88元/人份                                  

NG0558   痰液處理試劑盒     100人份/件    88元/人份                                  

NG0559   穿刺、刷檢細胞處理試劑盒         50人份/件       88元/人份                                  

NG0560   漿膜腔積液處理試劑盒         50人份/件       108元/人份                               

QKT0556  同型半胱氨酸/HCY酶法                  康特                                    

QKT0557  結(jié)核分枝桿菌抗體IgG檢測試劑盒酶聯(lián)免疫法                                              

QKT0558  丙型肝炎病毒核心抗原檢測試劑盒                                                  

9233299  Toothbelt, X-drive, P3xx1                   CNY                             

9233276  Motor, bar-code camera, Y-motor           CNY                             

9233272  LabHand motor (Plato 1***)            CNY                              【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】

9237711  #BR M48, Installation               CNY                             

9237164  Bezel XYP                   CNY                             

9237161  Adapter Vertical Height            CNY                             

9237121  Upgrade App., R.E.A.L. Prep 96                CNY                             

9237112  Upgrade App., QIAamp 96 Swab     Take advantage of our instrumentation ａnd molecular biology expertise to install QIAamp 96 DNA Swab on your BioRobot System.  The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN QIAamp 96 DNA Swab protocol.  Also included is a QIAamp 96 DNA Swab BioRobot 9604 Kit (12-plates) SAP #965842    CNY                             

9237103  Upgrade, BR plus 3 ext. devices       Upgrade BioRobot with three approved integration devices CNY                             

9236355  Sample Needle, short                CNY                             

9232936  Microplate Reader (UV-340nm)Module           CNY                             

9232915  Washer Board           CNY                             

9236325  Sample Valve             CNY                             

9230821  Rack for reagents,Troughs(for 6 troughs)                  CNY                             

4823024  careHIV-1 RT-PCR ASSAY           CNY                             

4861014  careEBV PCR ASSAY I                 CNY                             

130823     NeXtal CubicPhase 2w Mo/C 8% µplate (2)               CNY                             

130822     NeXtal CubicPhase 1w Mo/C 8% µplate (10)            CNY                             

130723     NeXtal Tubes Classics II Suite            CNY                              【人堿性成纖維細胞生長因子4(bFGF-4)ELISA試劑盒廠家】

4513363  artus HI Virus-1 QS-RGQ Kit (24) CE                  CNY                             

4510003  artus Enterovirus LC RT-PCR Kit (24) RUO                 CNY                             

9011277  Plate, ground, auto vac, BR8000              CNY                             

330504     RT² SYBR Green qPCR  Mastermix (6)              CNY                             

9010975  BGR 4er-Incubator PK80          CNY                             

133293     NeXtalStock Zinc acetate (200)                 CNY                             

1014051  Ni-NTA Silica (10 g)            CNY                             

1013342  Ni-NTA HisSorb Plates, black (100)            CNY                             

1005485  Q-Solution,5 x (2ml/2)                CNY                             

997012     Accessory Trough (10)               CNY                             

995999     Starter Pack, M48             CNY                             

674791     ipsogen ABL Control Gene 4 Standards             CNY                             

9240377  QIAcube, Installation       The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.  CNY                             

9239571  BR Universal, Basic Agreement       The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9239570  BR Universal, Premium Agreement         The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).    CNY                             

9020032  FOOTSET, RGQ                   CNY                             

9019986  PCB, small breakout, 12 core, C12            CNY                             

9019479  UPPER BUFFER LID GS              CNY                             

9238864  BR M48, Premium Agreement         The Premium Cover Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238852  LiquiChip, Basic Agreement     The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9241224  QIAxcel, Inspection Service      "Repair service delivery not applicable

Service response time (repair) not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: Yes, included" CNY                             

9241197  Silicone sealant, RTV 108, QX           CNY                             

9241183  Sensor, Y, with cable, QX           CNY                             

9233329  Belt, tooth, X-drive, BR8000             CNY                             

NG0473   19項凍干生化質(zhì)控血清                  支                                

NG0474   解脲支原體抗體IgG\IgM檢測試劑盒          血清         24T/48T                      

NG0475   沙眼衣原體抗原檢測試劑盒                   分泌物     20T                    

NG0476   沙眼衣原體抗體IgG\IgM檢測試劑盒          血清         24T/48T                      

NG0477   人型支原體抗體IgG\IgM檢測試劑盒          血清         24T/48T                      

NG0478   生殖支原體抗體IgG\IgM檢測試劑盒          血清         24T/48T                      

NG0479   淋球菌抗體IgG\IgM檢測試劑盒          血清         24T/48T                      

NG0480   梅毒抗體IgG\IgM檢測試劑盒               血清         24T/48T                      

NG0481   單純皰疹病毒Ⅱ抗體IgG\IgM檢測試劑盒          血清         24T/48T                      

NG0482   人類乳頭瘤病毒抗體HPV檢測試劑盒                  血清         24T/48T                      

NG0483   細菌性陰道病BV胺法           分泌物     20T                    

NG0484   梅毒特異性抗體試條檢測試劑盒                   血清         1T                      

NG0485   淋球菌抗原檢測試條檢測試劑盒                   分泌物     1T                      

NG0486   RC-J013，RPR膠乳法              血清         120T                            【人堿性成纖維細胞生長因子9(bFGF-9)ELISA試劑盒廠家】

NG0487   TPPA梅毒確診試劑膠乳法             分泌物     100T                           

NG0488   肺炎支原體抗體IgG\IgM檢測試劑盒          血清         20T/40T                      

NG0489   肺炎衣原體抗體IgG\IgM檢測試劑盒          血清         20T/40T                      

NG0490   結(jié)核抗體IgG\IgM檢測試劑盒               血清         20T/40T                      

NG0491   幽門螺桿菌抗體IgG\IgM檢測試劑盒          血清         20T/40T                      

NG0492   呼吸道合胞病毒抗體IgG\IgM檢測試劑盒          血清         20T/40T                      

NG0493   腺病毒抗體IgG\IgM\IgA檢測試劑盒           血清         20T/40T                      

NG0494   EB病毒衣殼抗體IgG\IgM\IgA檢測試劑盒          血清         20T/40T                      

NG0495   狂犬病毒抗體IgG\IgM檢測試劑盒               血清         20T/40T                      

NG0496   狂犬病毒抗原檢測試劑盒              血清         20T/40T                      

133220     NeXtalStock Sodium ac. H2O pH 5.0(200)                 CNY                              【人堿性成纖維細胞生長因子9(bFGF-9)ELISA試劑盒廠家】

301704     HiPerFect Transfection Reagent (0,5 ml)                   CNY                             

301427     Effectene Transfection Reagent (4x1ml) 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates      CNY                             

51326       QIAamp DNA Mini QIAcube Kit (240)                CNY                             

9012672  BGR Peripumpe PP-4 Frontbl.gerade (7135)            CNY                             

9002011  Rotor-Gene Q MDx 2Plex HRM no PC               CNY                             

292063     mericon Sheep Kit (24)              CNY                             

9001912  PyroMark Q24 MDx, PrioPLUS         "PyroMark Q24 MDx Instrument

Installation of PyroMark Q24  & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"         CNY                             

9001910  QIAgility HEPA/UV (w/o PC) PrioPLUS      "QIAgility HEPA/UV (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

133116     NeXtalStock Sodium thiocyanate (200)             CNY                             

967142     RNeasy 96 BioRobot 9604 Kit (12)  For 12 x 96 total ａnd cytoplasmic RNA preps on the BioRobot 9604: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents ａnd Buffers         CNY                             

962141     QIAprep 96 Turbo BioRobot Kit (4)  For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads         CNY                             

960241     QIAGEN Plasmid Plus 96 BioRobot Kit (4)                  CNY                             

51304       QIAamp DNA Mini Kit (50)       For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                             

978756     PyroMark CpG Assay 96 well (200)           CNY                             

9012712  Z-motor (Plato 7) left                 CNY                             

9012696  Set of X-flat band cable (Plato 7)               CNY                              【人堿性成纖維細胞生長因子9(bFGF-9)ELISA試劑盒廠家】

133239     NeXtalStock Sodium citrate pH 7.0 (200)                  CNY                             

133238     NeXtalStock Sodium citrate pH 5.8 (200)                  CNY                             

9002127  QIAsymphony RGQ, Priority    QIAsymphony RGQ Priority includes the QIAsymphony RGQ instruments, installations ａnd extended warranties. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

59203       EpiTect Whole Bisulfitome Kit (25)           CNY                             

59164       EpiTect Plus LyseAll Bisulfite Kit (48)                  CNY                             

929526     QX Alignment Marker 50 bp/1 kb (1.5 ml)                 CNY                             

133218     NeXtalStock MOPS pH 8.0 (200)                CNY                             

301605     RNAiFect Transfection Reagent (1ml)      RNAiFect Reagent ａnd buffer, for up to 170 transfections in 24-well plates; up to 500 transfections in 96-well plates  CNY                             

301525     TransMessenger Tranfect. Reagent(0.5ml)     For 60 transfections in 6-well plates or 80 transfections in 12-well plates         CNY                             

52304       QIAamp RNA Blood Mini Kit (50)     For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers    CNY                             

51306       QIAamp DNA Mini Kit (250)     For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                             

9002012  Rotor-Gene Q MDx 2plex HRM Platform                  CNY                             

970530     NRAS Pyro Kit (24)            CNY                             

970022     PyroMark Q24 CpG MLH1 (4 x 24)   For quantification of methylation level in region -209 to -188 from the transcription start site. CNY