1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】齊一生物科技歡迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標記物如酶標結(jié)合物則通過各種化學合成方法制備.近年來,隨著分子生物學的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性. 【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x2185 PC-3M 1E8 前列腺癌細胞高轉(zhuǎn)移亞系                                                      

QY-x2186 PC-3M 2B4 前列腺癌細胞低轉(zhuǎn)移亞系                                                      

QY-x2187 PG49 人肺腺癌細胞系                                                       

QY-x2188 PG-BE1 人肺巨細胞癌(PG)的高轉(zhuǎn)移亞系                                                

QY-x2189 PG-LH7 人肺巨細胞癌(PG)的低轉(zhuǎn)移亞系                                                

QY-x2190 RL-952 子宮內(nèi)膜癌細胞株                                                

QY-x2191 SGH44 人腦膠質(zhì)細胞瘤細胞系                                                 

QY-x2192 TJ905 人腦惡性膠質(zhì)瘤細胞系                                                  

QY-x2193 U-373MG 人腦膠質(zhì)瘤細胞系                                                    

QY-x2194 HUVEC 原代 人臍靜脈內(nèi)皮細胞                                                       【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】

QY-x2195 Sp2/0 小鼠骨髓瘤細胞系                                                   

QY-x2196 ECV304 正常內(nèi)皮細胞系                                                   

QY-x2197 ACC-M人唾液腺性肺癌高轉(zhuǎn)移細胞                                                   

QY-x2198 HEP2人喉癌細胞                                                 

QY-x2199 H7402 肝癌細胞                                                  

QY-x2200 HEPG2人肝癌細胞                                                       

QY-x2201 PG5人肝癌細胞                                                   

QY-x2202 NCL-H446 人肺癌細胞                                                

QY-x2203 22RV1 人前列腺癌細胞                                                      

QY-x2204 293 人胚腎細胞                                                  

QY-x2205 293T 人胚腎細胞                                                

QY-x2206 2BS 人胚肺成纖維樣細胞                                                  

QY-x2207 311 果蠅胚胎細胞                                                       

QY-x2208 3T3-L1 Swiss albion小鼠脂肪細胞                                              

QY-x2209 3T6 Swiss albion小鼠胚胎成纖維細胞                                               

QY-x2210 801-D 人大細胞肺癌細胞                                                    【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】

QY-x2211 A375 人惡性黑色素瘤細胞                                                

QY-x2212 A549 人小細胞肺癌細胞                                                    

QY-x2213 AGS 人胃腺癌細胞                                                      

QY-x2214 Ana-1 小鼠巨噬細胞                                                   

QY-x2215 AtT20 小鼠垂體瘤細胞                                                       

QY-x2216 B16 小鼠黑色素瘤細胞                                                       

QY-x2217 B95-8 絨猴EBV轉(zhuǎn)化的淋巴細胞                                                       

9238645  Brake resistor 112 Ohm / 100 W               CNY                             

9242061  Pipetting container, IQ/OQ, QS                 CNY                             

9241827  Pyro Q96 ID, Premium Agreement "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241166  Board, A/D, QX                  CNY                             

9240955  Sensor, z, handler, Qsym           CNY                             

9240835  QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.  CNY                             

9240766  32 OUT Module, D2-32TD1, AP96            CNY                             

6000-5018        MST STM Rack & Lid (Blue - 2 pos. cal.)            CNY                             

9241777  QIAxtractor, Inspection Service       "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY                             

9241375  Magnet, door lock, top, EZ1a           CNY                             

9241334  Keypad foil, EZ1                 CNY                             

9240750  Belt, Elevator, AP              CNY                             

9243648  Acceler. Valid Supp, Other Extract Sys     The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).          CNY                             

9243553  #BR M96, PM Service      "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                              【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】

9242864  QIArent&go -Rent out fee QSSP/AS Cabinet             CNY                             

5000-1321        Feet Set/4, SKR1                CNY                             

5000-1288        DML 2000 - Fuse 160 mA PKG 10              CNY                             

5000-1287        DML 2000 - Fuse 315 mA PKG 10              CNY                             

5000-1505        Switch ON-OFF 120V, HTR1               CNY                             

6000-00181      Plate Washer - Replacement Tubing Kit           CNY                             

6000-00178      Plate Washer - Maintenance Kit               CNY                             

5100-00047      Wash Buffer Concentrate (2 Bottles)       "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"      CNY                             

9240740  Tubing, Tygon, 1/4" ID                CNY                             

9240735  Tubing, Black, Capper, AP196/98               CNY                             

9240724  Tube Assy, Draw, AP96/196                CNY                             

5000-1412        Poteneiometer 500K, VTX2               CNY                             

9240506  Monitor, 17", Flat Panel, AP             CNY                             

9239851  Cover, LC200             CNY                             

9239565  xMAP Alignment Microspheres                 CNY                             

9019942  Barrel removal tool, CX1          CNY                              【人單核細胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒廠家】

9019474  Lid, UV, polycarb, C1x                CNY