1.購買我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請(qǐng)勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲(chǔ)。 【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號(hào)的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開始之前，清潔工作臺(tái)。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對(duì)收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，請(qǐng)?jiān)炷Ｈ〔暮�，將�?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請(qǐng)?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

QY-x2086 HIH 3T3 小鼠胚胎成纖維細(xì)胞 1640+10%胎牛血清                                                

QY-x2087 L929 小鼠成纖維細(xì)胞 1640+10%胎牛血清                                                      

QY-x2088 HSC-T6 大鼠肝星狀細(xì)胞 1640+10%胎牛血清                                                  

QY-x2089 L-02 人肝細(xì)胞 1640+10%胎牛血清                                                   

QY-x2090 SRA01/04 人晶體上皮細(xì)胞 MEM+非必需氨基酸＋10%胎牛血清                                                  

QY-x2091 CHO 中國倉鼠卵巢細(xì)胞（野生型） 1640+10%胎牛血清                                                       

QY-x2092 IEC-6 大鼠小腸隱窩上皮細(xì)胞 DMEM+10%胎牛血清                                                      

QY-x2093 P815 小鼠肥大細(xì)胞 1640+10%胎牛血清                                                 

QY-x2094 NRK-49F 大鼠正常腎成纖維細(xì)胞 1640+10%胎牛血清                                                    

QY-x2095 自建 正常成人皮膚成纖維細(xì)胞 DMEM+20%胎牛血清 原代培養(yǎng)                                                

QY-x2096 HELF 人胚肺成纖維細(xì)胞 1640+10%胎牛血清 5，6代                                                   

QY-x2097 293 人胚腎細(xì)胞 DMEM+10%胎牛血清                                                    

QY-x2098 293T 人胚腎T細(xì)胞 1640+10%胎牛血清                                                   【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】

QY-x2099 GES-1 人胃粘膜細(xì)胞 DMEM+10%胎牛血清                                                     

QY-x2100 3T3-L1 小鼠胚胎成纖維細(xì)胞（前脂肪） DMEM+10%胎牛血清                                                   

QY-x2101 Vero 猴腎細(xì)胞 1640+10%胎牛血清                                                  

QY-x2102 CHO-dhFr- 中國倉鼠卵巢細(xì)胞(二氫葉酸缺陷型） 1640+10%胎牛血清                                               

QY-x2103 V79 中國倉鼠肺細(xì)胞 1640+10%胎牛血清                                               

QY-x2104 PA317 小鼠胚胎成纖維細(xì)胞 1640+10%胎牛血清                                                    

QY-x2105 Cos-7 綠猴腎細(xì)胞 DMEM+10%胎牛血清                                                 

QY-x2106 MA104 非洲綠猴胚胎腎上皮細(xì)胞 DMEM+10%胎牛血清                                                       

QY-x2107 16HBE 人支氣管上皮細(xì)胞 1640+10%胎牛血清                                                       

QY-x2108 Marc145 猴胚胎腎上皮細(xì)胞                                                      

QY-x2109 MDA-MB-435S 人乳腺導(dǎo)管癌細(xì)胞                                                     

QY-x2110 MDBK 瘤牛腎細(xì)胞                                                       

QY-x2111 MEG-01 人成巨核細(xì)胞白血病細(xì)胞                                                   

QY-x2112 MFC 小鼠胃癌細(xì)胞                                                      

QY-x2113 MG-63 人骨肉瘤細(xì)胞                                                 

QY-x2114 MLA144長臂猿淋巴瘤細(xì)胞                                                        【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】

QY-x2115 MouseM 小鼠肌肉細(xì)胞                                                       

QY-x2116 MouseS 小鼠皮膚成纖維樣細(xì)胞                                               

QY-x2117 Hep G2人肝癌細(xì)胞系                                                

QY-x2118 Hep3B人肝癌細(xì)胞系                                                 

9013170  Tubing, syringe inlet 2, BR8000                 CNY                             

9011417  Klemmplättchen Varispan P7 (Alodine)             CNY                             

5050-1020EN   #Keyboard English Spare          CNY                             

9240731  Tubing, 3/8"OD , Blue, AP                   CNY                             

676091     ipsogen CBFB-MYH11 A Standards           CNY                             

9011341  Microplate Washer Module Plato 3                   CNY                             

9011203  Vacuum control unit, BR8000           CNY                             

673522     ipsogen JAK2 MutaQuant Kit (12) CE                 CNY                             

337839     qBiomarker SYBR Fluor Mastermix 25 ml                  CNY                             

337632     qBiomarker SYBR Green ROX Mastermix (12)                   CNY                             

9018907  36-Well Rotor            CNY                              【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】

9018783  CAT, Qsym                  CNY                             

9018694  EZ1 Adv. XL DNA Bacteria Card                 CNY                             

9018565  Door lock, mechanical, EZ1a            CNY                             

9013132  Elevating Platform            CNY                             

4509063  artus WNV LC RT-PCR Kit (24) CE              CNY                             

9001905  #QIAgility (w/o PC) System       QIAgility (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                             

800383     Open Reading Frame Search            CNY                             

9019052  Notebook 2 XP          CNY                             

9020200  ZLV PCB, USB, 18Q            CNY                             

9020580  Cable, DB9 M/F (UL) 6ft, DML 3000                  CNY                             

9020588  Pulley, belt, DML 3000              CNY                             

9021016  Robot Stage, X Axis, VR, DCU           CNY                             

9013376  Maintenance Kit BR 8000 Research                  CNY                             

9013279  BGR Upgrade-Kit Scanner PK80                CNY                             

9013272  Peristaltic Pump, RCS1              CNY                             

9013190  Motor, rotor, buffer track, BR8000          CNY                              【人巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒現(xiàn)貨】

132772     NeXtal Stock Jeffamine M-600 (200)        100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)       CNY                             

132620     NeXtal Stock Tricine (50) 1M Tricine - 50mL (4 x 12,5 ml tube)        CNY                             

132560     NeXtal Stock Magnesium chloride (50)    2M Magnesium chloride hexahydrate - 50mL (4 x 12,5 ml tube)    CNY                            

800171     Easy-Read Tubes, w/o prep      "Sequencing of plasmid DNA ａnd PCR products

DNA quality control not included

No repetition of sequencing

Data collected to maximal read length" CNY                             

QY-x2083 PC-12 嗜鉻細(xì)胞瘤 1640+10%胎牛血清+5%馬血清 體外培養(yǎng)株                                                    

QY-x2084 [編輯本段]細(xì)胞庫部分細(xì)胞系目錄（遺傳和正常細(xì)胞）                                                

QY-x2085 細(xì)胞名稱 組織類型 培養(yǎng)條件                                                 

5000-1311        Capacitor 8UF, SKR1                  CNY                             

5000-1273        Door Damper Assembly, DML2                 CNY                             

5000-1258        Y Optical Sensor CMPLT, DML2                  CNY                             

5050-1074 V.1 CD Rom Manual - LumiCheck Plate          CNY                             

5050-1069 V.1 CD Rom Manual - Rotary Shaker I, 240V                   CNY                             

6000-5001        Costar Plate Lids                CNY                             

6000-3120        Plate Washer I - WasteReserv.CapAssembly             CNY                             

5000-1420        Driveplate Asmly 099A, VTX2           CNY                             

5127-1220        hc2 Sample Conversion Kit                CNY                             

9243701  careHPV, Training             CNY                              【人巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

EA11423  EASYartus BKV RG_I                  CNY                             

800190     Sequencing in a Box Service              CNY                             

800110     Single Read, w/ prep        "Bacterial clone cultivation ａnd template purification or PCR product purification, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length" CNY                             

132538     NeXtal Stock Dioxane (50)        100% v/v Dioxane - 50mL (5 x 10 ml tube)       CNY                             

338162     RT2 PCR Array Loading Reservoir             CNY                             

338114     Mouse XpressRef Universal Total RNA              CNY                             

203743     QIAGEN Fast Cycling PCR Kit (200)  For 200 x 20 µl reactions: 2 x 1 ml Fast Cycling Master Mix, 2x (containing dNTPs, patent pending buffer composition), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution CNY                             

180820     GeneRead qPCR SYBR Green MM (2)               CNY                             

159320     Desicant (1000)                 CNY                             

159142     QIAsafe DNA Blood 48-Well Plates (10)            CNY                             

158822     Gentra Puregene Buccal Cell Kit (10)                CNY                             

158689     Gentra Puregene Tissue Kit (33 g)            CNY                             

678691     ipsogen MLL-AF9 type A e10e6 Standards                CNY                             

32943       cis-Repressed pQE-Kan Vector Set            CNY                             

32506       EasyXpress Protein Synthesis Maxi Kit     For reactions up to 4000 ul: 4 x 350 ul E. coli extract, reaction buffer, RNase-free water, ａnd positive-control DNA     CNY                             

30930       Glutathione HiCap Matrix (100 ml)          CNY                             【人巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

30725       #Ni-NTA Superflow Cartridges (100x1 ml)                 CNY                             

30210       Ni-NTA Agarose (25 ml)   25 ml nickel-charged resin (max. pressure: 2.8 psi)          CNY                             

28183       QIAquick 96 PCR Purification Kit (24)       For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps        CNY                             

9011608  BGR Kamm für Stirrer PK80 neu                CNY                             

9013178  Gripper set, BR8000 v 1.00               CNY