齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標準和樣品的重復？

是的，重復的運行，以監(jiān)測測定的精度和提高檢測結果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數(shù)量的樣品。

3。什么類型的可重復性的結果，得到與分析？

每一個工具都有一個包含一個典型數(shù)據(jù)的圖表的手冊。在運營商的任何變化，移液和洗滌技術，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結果。每個用戶都應該得到自己的標準曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應該如何儲存我的樣品？

樣品應保存在-20°C或更低的溫度。長期儲存，建議凍結他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進行了優(yōu)化，提供*佳的結果。修改的格式或協(xié)議可能會給出錯誤的結果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經(jīng)檢驗的樣品類型。為進一步信息的聯(lián)系技術服務。

8。我的樣本產(chǎn)生的值，在動態(tài)范圍內(nèi)的測定。我可以使用這些值嗎？

建議只有在標準曲線的范圍內(nèi)下降的樣本值。標準曲線的范圍內(nèi)的值一般是非線性的，這可能導致不正確的外推值。產(chǎn)生值高于標準的樣品應為（進一步）稀釋和重復。如果樣品低于該測定的范圍內(nèi)，該樣品被認為是不可檢測的。

9。我每次都要運行一個空白或零標準嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設計為*佳的性能在給定的體積

11。可以使用不同的組件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進行質(zhì)量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應商。

12。我的標準曲線看起來很好，但我沒有得到一個信號，我的樣本，當我預期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導致樣品在標準曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

14。我需要用一個盤子嗎？

可靠的結果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預期的分析物濃度，我應該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯(lián)網(wǎng)在多個科學數(shù)據(jù)庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

QY-x2053 細胞中心實驗室細胞庫部分細胞系目錄（鼠類腫瘤細胞）                                                  

QY-x2054 1.小鼠類                                               

QY-x2055 細胞名稱 組織類型 培養(yǎng)條件                                                 

QY-x2056 Lewis 肺癌 瘤株                                                 

QY-x2057 LA795 肺腺癌 瘤株                                                     

QY-x2058 EAC 艾氏腹水瘤 瘤株                                                

QY-x2059 S180 腹水瘤 瘤株                                                       

QY-x2060 H22 肝癌 瘤株                                                    

QY-x2061 H22 肝癌 1640+10%胎牛血清 體外培養(yǎng)株                                                     

QY-x2062 MPC-83 胰腺腺泡細胞癌 1640+10%胎牛血清 體外培養(yǎng)株                                                   

QY-x2063 MPC-83 胰腺腺泡細胞癌 瘤株                                                  

QY-x2064 RIN-m5F β胰島素瘤 1640+10%胎牛血清 體外培養(yǎng)株                                                 

QY-x2065 MA891 乳腺癌 瘤株                                                   

QY-x2066 TA2 自發(fā)高乳腺癌 瘤株                                                      【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

QY-x2067 U14 宮頸癌 瘤株                                               

QY-x2068 YAC-1 淋巴瘤 1640+10%胎牛血清 體外培養(yǎng)株                                                       

QY-x2069 Nb2－11 淋巴瘤 體外培養(yǎng)株                                                    

QY-x2070 C26 結腸癌 瘤株                                                

QY-x2071 G422 神經(jīng)膠質(zhì)瘤 瘤株                                                      

QY-x2072 B16 黑色素瘤 1640+10%胎牛血清 體外培養(yǎng)株                                                      

QY-x2073 B16 黑色素瘤 瘤株                                                    

QY-x2074 C3 白血病 體外培養(yǎng)株                                                       

QY-x2075 L1210 淋巴白血病 1640+10%胎牛血清 體外培養(yǎng)株                                                      

QY-x2076 L1210 淋巴白血病 瘤株                                                     

QY-x2077 SP2/0 骨髓瘤 1640+10%胎牛血清 體外培養(yǎng)株                                               

QY-x2078 NS-1 骨髓瘤 1640+10%胎牛血清 體外培養(yǎng)株                                                

QY-x2079 P388D 淋巴母細胞瘤 1640+10%胎牛血清 體外培養(yǎng)株                                                 

QY-x2080 Kert-3 腎癌 瘤株                                                

QY-x2081 TC-1 HPVl6 E6、E7和ras基因共轉化的C57BL／C(H-2b)小鼠肺上皮細胞 1640+10%胎牛血清體外培養(yǎng)株                                                       

QY-x2082 W256 肉瘤(Wistar) 瘤株                                                     【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

QY-x2083 PC-12 嗜鉻細胞瘤 1640+10%胎牛血清+5%馬血清 體外培養(yǎng)株                                                    

QY-x2084 [編輯本段]細胞庫部分細胞系目錄（遺傳和正常細胞）                                                

QY-x2085 細胞名稱 組織類型 培養(yǎng)條件                                                 

5000-1311        Capacitor 8UF, SKR1                  CNY                             

5000-1273        Door Damper Assembly, DML2                 CNY                             

5000-1258        Y Optical Sensor CMPLT, DML2                  CNY                             

5050-1074 V.1 CD Rom Manual - LumiCheck Plate          CNY                             

5050-1069 V.1 CD Rom Manual - Rotary Shaker I, 240V                   CNY                             

6000-5001        Costar Plate Lids                CNY                             

6000-3120        Plate Washer I - WasteReserv.CapAssembly             CNY                             

5000-1420        Driveplate Asmly 099A, VTX2           CNY                             

5127-1220        hc2 Sample Conversion Kit                CNY                             

9243701  careHPV, Training             CNY                              【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

EA11423  EASYartus BKV RG_I                  CNY                             

800190     Sequencing in a Box Service              CNY                             

800110     Single Read, w/ prep        "Bacterial clone cultivation ａnd template purification or PCR product purification, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length" CNY                             

132538     NeXtal Stock Dioxane (50)        100% v/v Dioxane - 50mL (5 x 10 ml tube)       CNY                             

338162     RT2 PCR Array Loading Reservoir             CNY                             

338114     Mouse XpressRef Universal Total RNA              CNY                             

203743     QIAGEN Fast Cycling PCR Kit (200)  For 200 x 20 µl reactions: 2 x 1 ml Fast Cycling Master Mix, 2x (containing dNTPs, patent pending buffer composition), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution CNY                             

180820     GeneRead qPCR SYBR Green MM (2)               CNY                             

159320     Desicant (1000)                 CNY                             

159142     QIAsafe DNA Blood 48-Well Plates (10)            CNY                             

158822     Gentra Puregene Buccal Cell Kit (10)                CNY                             

158689     Gentra Puregene Tissue Kit (33 g)            CNY                             

678691     ipsogen MLL-AF9 type A e10e6 Standards                CNY                             

32943       cis-Repressed pQE-Kan Vector Set            CNY                             

32506       EasyXpress Protein Synthesis Maxi Kit     For reactions up to 4000 ul: 4 x 350 ul E. coli extract, reaction buffer, RNase-free water, ａnd positive-control DNA     CNY                             

30930       Glutathione HiCap Matrix (100 ml)          CNY                             【人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒廠家】

30725       #Ni-NTA Superflow Cartridges (100x1 ml)                 CNY                             

30210       Ni-NTA Agarose (25 ml)   25 ml nickel-charged resin (max. pressure: 2.8 psi)          CNY                             

28183       QIAquick 96 PCR Purification Kit (24)       For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps        CNY                             

9011608  BGR Kamm für Stirrer PK80 neu                CNY                             

9013178  Gripper set, BR8000 v 1.00               CNY