【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒廠家】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒廠家】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒廠家】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x1723       狗腎細(xì)胞       MDCK           狗腎臟    DMEM＋10％NBCS           

QY-x1724       人胚腎細(xì)胞（野生型）       293T-rex-wild-M2         人胚腎    DMEM＋10％NBCS           

QY-x1725       人胚腎細(xì)胞（31位點(diǎn)突變）      293T-rex-S 31N            人胚腎    DMEM＋10％NBCS           

QY-x1726       人胃腺癌細(xì)胞       MCG803                            

QY-x1727       人胃腺癌細(xì)胞       SCG-7901                                 

QY-x1728       人胃癌細(xì)胞(未分化)     HGC-27                             

QY-x1729       人結(jié)腸癌細(xì)胞       Lovo                                  

QY-x1730       人結(jié)腸癌細(xì)胞       SW480                                【人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒廠家】

QY-x1731       人肝細(xì)胞癌細(xì)胞    BEL-7402                                  

QY-x1732       人肝細(xì)胞癌細(xì)胞    SMMC-7721                              

QY-x1733       人肝癌細(xì)胞   QGY- 7701                                

QY-x1734       人肝癌細(xì)胞    Hep-3b                               

QY-x1735       轉(zhuǎn)染四環(huán)素正向調(diào)控表達(dá)基因7402細(xì)胞  BEL-7402-Tet-on                              

QY-x1736       轉(zhuǎn)染綠色熒光蛋白和四環(huán)素正向調(diào)控表達(dá)基因7402細(xì)胞      7402-Tet-on-EGFP                            

QY-x1737       人胰腺癌細(xì)胞       Panc-1                               

QY-x1738       人胰腺癌細(xì)胞       SW1990                             

QY-x1739       人口腔上皮癌細(xì)胞       KB                              

QY-x1740       人口底癌細(xì)胞耐藥株［長春新堿（VCR）誘導(dǎo)高表達(dá)（p-gp）］   KV                              

QY-x1741       人肺巨細(xì)胞癌細(xì)胞       HLAmP                              

QY-x1742       人肺腺癌細(xì)胞       GLC-82                              

QY-x1743       人肺腺癌細(xì)胞（轉(zhuǎn)染TK基因的GLC-82細(xì)胞）      GLC-82-TK                               

QY-x1744       人鼻咽癌細(xì)胞（高分化）    CNE-1                               

QY-x1745       人鼻咽癌細(xì)胞       SUME-a                             

QY-x1746       人鼻咽癌細(xì)胞       HNE-2                                【人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒廠家】

QY-x1747       人慢性髓原白血病細(xì)胞       K562                                  

QY-x1748       人組織細(xì)胞淋巴瘤細(xì)胞       U937                                  

QY-x1749       人紅白細(xì)胞白血病細(xì)胞Erythroleukemia    HEL                                  

QY-x1750       人急性T淋巴母細(xì)胞白血病細(xì)胞       MOLT-4                             

QY-x1751       人急性早幼粒白血病細(xì)胞（懸�。�    NB4                            

QY-x1752       人骨肉瘤細(xì)胞       SW1353                             

QY-x1753       人白血病細(xì)胞       Daudi                                 

QY-x1754       轉(zhuǎn)染了tk基因的SW038-C2細(xì)胞       SW038-C2-tk                            

QY-x1755       人星形膠質(zhì)細(xì)胞瘤細(xì)胞      SHC-44                              

9240716  Lead Screw Assy, Capper U\D, AP196/98           CNY                           

9240712  Muffler, Elevator Brake, AP196/98              CNY                           

5000-1416      Switch LRGSK61RCBE250, VTX2             CNY                           

6000-5023      LumiCheck Plate User Package (CD Manual)             CNY                           

6000-3102      Rapid Capture System & Manuals        CNY                           

12941     QIAGEN Plasmid Plus Midi Sample Kit (5)  QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction. CNY                           

9240695  Needle Valve, Flow Control, AP196/98         CNY                           

9002141  careHPV test Shaker             CNY                           

273501    pigtype Trichinella Ab (1)             CNY                           

273803    pigtype Yersinia Ab (5)          CNY                           

280433    virotype BTV RT-PCR Kit (24)            CNY                           

281607    virotype SBV RT-PCR Kit (480)           CNY                           

800371    Shipping,Postage,Packaging (stand. mail)            CNY                           

9019497  ZLV MANUAL CAS1200              CNY                           

9020112  Accessory power lead (vac. station), QXT          CNY                           

9020578  Adapter, USB to Serial, DML 3000              CNY                            【人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒廠家】

9020587  Pulley, motor, DML 3000             CNY                           

9020595  Rubber Foot, 2" Dia x 1" Ht, DML 3000             CNY                           

9232722  Cooling block, 96-tube 0.2 ml       Holder for accommodating 96-well amplification plate (0.2 ml per well) on the worktable       CNY                           

9013265  Cable, 24V, power to cooling, 1900 mm              CNY                           

9013257  Tubing set , HSPS, 90/120,0        CNY                           

9013250  BGR Waste System              CNY                           

9013216  Screws, plastic, M4 (20)              CNY                           

9013202  Valve set, throttle, auto vac, BR8000            CNY                           

9011758  Reagent holder, 8-tube 1.5 ml       Holder for accommodating 8 microcentrifuge tubes (1.5 ml) on the worktable; must be used with the reagent holder tray   CNY                           

132625    NeXtal Stock TRIS. HCl (50)       1M Tris(hydroxymethyl)aminomethane HCl - 50mL (4 x 12,5 ml tube)     CNY                           

9241374  Magnet, door lock, bottom, EZ1a         CNY                           

9241373  Cable, key panel board, EZ1a        CNY                           

9240752  Downtube, Reagent #2-6, AP98           CNY                           

9240751  Belt, TR,Capper U/D & I/O, AP           CNY                           

9243546  QIAsymphony SP AS, PM Service       "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY