齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶在不同的時(shí)間來(lái)分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營(yíng)商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長(zhǎng)期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類型。未經(jīng)檢驗(yàn)的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問(wèn)題的來(lái)源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來(lái)沒(méi)有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來(lái)很好，但我沒(méi)有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請(qǐng)檢查是否正確進(jìn)行稀釋。過(guò)稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無(wú)塵紙。【人可溶性CD28(sCD28)ELISA試劑盒廠家】

14。我需要用一個(gè)盤子嗎？

可靠的結(jié)果可以得到無(wú)板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長(zhǎng)校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長(zhǎng)讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過(guò)程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來(lái)源。這個(gè)信息的*佳來(lái)源是目前的文獻(xiàn)，很容易通過(guò)互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫(kù)訪問(wèn)。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來(lái)了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人可溶性CD28(sCD28)ELISA試劑盒廠家】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人可溶性CD28(sCD28)ELISA試劑盒廠家】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人可溶性CD28(sCD28)ELISA試劑盒廠家】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

QY-HC093         人腎小管上皮細(xì)胞系HK-2    HK-2                                              

QY-HC094         人子宮內(nèi)膜腺癌細(xì)胞 HEC-1-A      HEC-1-A                                        

QY-HC095         人子宮內(nèi)膜腺癌細(xì)胞 HEC-1-B      HEC-1-B                                         【人可溶性CD28(sCD28)ELISA試劑盒廠家】

QY-HC096         人Ishikawa子宮內(nèi)膜癌細(xì)胞 ISK  ISK                                        

QY-HC097         人子宮內(nèi)膜腺癌細(xì)胞 AN3CA        AN3CA                                          

QY-HC098         人神經(jīng)母細(xì)胞瘤細(xì)胞 IMR-32       IMR-32                                         

QY-HC099         人皮膚成纖維細(xì)胞 HSF         HSF                                       

QY-HC0100       人Burkitt淋巴瘤細(xì)胞 RAJI   RAJI                                      

QY-HC0101       人B淋巴細(xì)胞急性白血病細(xì)胞 BALL-1        BALL-1                                          

QY-HC0102       人宮頸癌細(xì)胞 Hela-s3  Hela-s3                                         

QY-HC0103       人卵巢癌細(xì)胞 HO-8910         HO-8910                                      

QY-HC0104       人腎小球系膜細(xì)胞 HMC       HMC                                              

QY-HC0105       人小細(xì)胞肺癌細(xì)胞 NCI-H446        NCI-H446                                     

QY-HC0106       人骨髓瘤細(xì)胞/人漿細(xì)胞白血病細(xì)胞 NCI-H929         NCI-H929                                     

QY-HC0108       人胚肺成纖維細(xì)胞 HELF       HELF                                              

QY-HC0109       人飼養(yǎng)層上皮細(xì)胞 HPT-8     HPT-8                                            

QY-HC0110       人非小細(xì)胞肺癌細(xì)胞人肺支氣管癌細(xì)胞 NCI-H1650　     NCI-H1650　                                       

QY-HC0111       人大細(xì)胞肺癌細(xì)胞 NCI-H661　   NCI-H661　                                          

QY-HC0112       人外周血嗜堿性白血病細(xì)胞 KU812    KU812                                            【人可溶性CD28(sCD28)ELISA試劑盒廠家】

QY-HC0113       人乳腺導(dǎo)管癌細(xì)胞 MDA-MB-435         MDA-MB-435                                      

QY-HC0114       人骨髓瘤細(xì)胞 OPM-2 OPM-2                                          

QY-HC0115       人腺癌細(xì)胞 F56     F56                                       

QY-HC0116       人SV－40轉(zhuǎn)化肺成纖維細(xì)胞 WI-38VA13   WI-38VA13                                           

QY-HC0117       人肺癌細(xì)胞 Calu-1         Calu-1                                           

QY-HC0118       人腎透明細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞 Caki-1          Caki-1                                            

QY-HC0119       人胰腺癌細(xì)胞 SW 1990         SW1990                                        

QY-HC0120       人胰腺癌細(xì)胞 CFPAC-1 CFPAC-1                                        

QY-HC0121       人非雄激素依賴型前列腺癌細(xì)胞 Tsu-Pr1   Tsu-Pr1                                         

QY-HC0122       人急性淋巴母細(xì)胞白血病細(xì)胞 MT-4  MT-4                                             

QY-HC0123       人卵巢癌細(xì)胞 NIH/OVCAR-3         NIH/OVCAR-3                                      

QY-HC0125       人喉癌細(xì)胞 TU 686        TU686                                           

QY-HC0126       人喉癌細(xì)胞 TU 212        TU212                                           

QY-HC0127       人喉癌細(xì)胞 M2e M2e                                     

9019603  Pins, locating, Mix ａnd Reagent set, CG1                  CNY                             

9019598  Lead, vac trap sensor, CX1                 CNY                             

9019573  Belt, X-axis assembly, CQx                  CNY                             

9019546  Clamp, cable, 3 wires 2 tabs, Cxx              CNY                             

9019533  PCB, USB, QGY                   CNY                              【人可溶性CD28(sCD28)ELISA試劑盒廠家】

9019518  RISER VACUUM 16.25MM                CNY                             

217004     miRNeasy Mini Kit (50)             CNY                             

204243     QuantiTect SYBR Green RT-PCR Kit (200)         For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect SYBR Green RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water        CNY                             

9019439  Heating block assy            CNY                             

9019425  Gasket, lower chamber, RG3            CNY                             

9019416  Box, shipping with foam packing, RG3              CNY                             

9019401  Connector, COM incl. int. cable, RG3                CNY                             

9019360  Switch, mains voltage selector, RG6                 CNY                             

1027417  Flexitube siRNA 5 nmol             CNY                             

9019339  LED, green (530), RG6              CNY                             

9019331  EPROM, IC Motherbrd, state FW, RG6             CNY                             

9019237  Linear guide - X, PyroQ24                  CNY                             

9234673  X-Assembly Turntable Card                CNY                             

9234659  Cassette, HSPS, click＇n＇go                  CNY                             

1026956  Buffer AVE (Elution Buffer) (125/125),KG          CNY                             

1026905  RNeasy 96 Plates (12)                CNY                              【人可溶性CD28(sCD28)ELISA試劑盒廠家】

9233674  Probe, ceramic coat., 0.4mm, 158mm             CNY                             

9233630  Mousepad BR 01/10 (WW)                 CNY                             

9019209  Motor - Gripper, PyroQ96MD           CNY                             

9019197  Damper kit - Robot, PyroQ96MD              CNY                             

1020955  Buffer AW2 with Azid (127/500),KG           CNY                             

1062791  HBV PCR REAGENT III               CNY                             

9232903  Spritzenpumpe mMBP 2021C P7xxx                  CNY                             

1061065  PG Influenza A RT-PCR Assay (48) Z          CNY                             

9232900  Valve, release, auto vac, BR8000             CNY                             

9016710  Software Package LiquiChip Analyzer               CNY